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Proc Natl Acad Sci U S A. 1995 Nov 7; 92(23): 10526–10530.
doi: 10.1073/pnas.92.23.10526
PMCID: PMC40644
PMID: 7479833

The nuclear matrix protein NMP-1 is the transcription factor YY1.

B Guo, P R Odgren, A J van Wijnen, T J Last, J Nickerson, S Penman, J B Lian, J L Stein, and G S Stein
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Abstract

NMP-1 was initially identified as a nuclear matrix-associated DNA-binding factor that exhibits sequence-specific recognition for the site IV regulatory element of a histone H4 gene. This distal promoter domain is a nuclear matrix interaction site. In the present study, we show that NMP-1 is the multifunctional transcription factor YY1. Gel-shift and Western blot analyses demonstrate that NMP-1 is immunoreactive with YY1 antibody. Furthermore, purified YY1 protein specifically recognizes site IV and reconstitutes the NMP-1 complex. Western blot and gel-shift analyses indicate that YY1 is present within the nuclear matrix. In situ immunofluorescence studies show that a significant fraction of YY1 is localized in the nuclear matrix, principally but not exclusively associated with residual nucleoli. Our results confirm that NMP-1/YY1 is a ubiquitous protein that is present in both human cells and in rat osteosarcoma ROS 17/2.8 cells. The finding that NMP-1 is identical to YY1 suggests that this transcriptional regulator may mediate gene-matrix interactions. Our results are consistent with the concept that the nuclear matrix may functionally compartmentalize the eukaryotic nucleus to support regulation of gene expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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