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FIGURE 3.

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Metal analysis of B. burgdorferi versus iron-philic organisms and effects on SodA activity. A, ICP MS analysis of manganese and iron was carried out with whole cell B. burgdorferi strain ML23 versus E. coli cells grown in BSK medium, as described under “Experimental Procedures.” B, AAS measurements of manganese in soluble protein lysates from B. burgdorferi (Bb) strain ML23, E. coli, and S. cerevisiae as described under “Experimental Procedures.” C, AAS analysis of whole cell manganese in B. burgdorferi strain 297 and the corresponding bmtA mutant compared with E. coli (top) and B. burgdorferi strain ML23 grown in BSK supplemented with the indicated concentrations of MnCl2 (bottom). D, SodA activity and protein levels were examined as in Fig. 1A in B. burgdorferi strain 297 and the corresponding bmtA mutant (top) and B. burgdorferi strain ML23 grown with the indicated concentrations of MnCl2 (bottom).

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