FIGURE 2.
Multidimensional chromatography of B. burgdorferi lysates. Soluble B. burgdorferi lysates were resolved by anion exchange, and the 300 and 400 mm NaCl elutions were resolved by size exclusion (SE) chromatography; shown are results from fractions 11–22 of increasing retention time on size exclusion. A, fractions were subjected to either metal analysis by ICP-MS (Fe and Mn), where y axis units represent relative abundance, or proteomic analysis by trypsin digestion, LC/MS, and MS/MS (SodA and A-peptidase), where y axis units represent spectral counts. Shaded boxes indicate manganese peak overlaps with SodA and with amino peptidase-1 (gene BB0366). B, fractions were analyzed for SodA activity by the native gel assay. C, proteomic analysis of fractions to illustrate that the manganese in fraction 19 shows poor correlation with a fructose bisphosphate aldolase (F-bP aldolase gene BB0445) and elongation factor EF-2 (EF-2 gene BB0540).









