Figure 3

A, Bivariate distribution of c-kit, IGF-1R and IGF-2R in hCSCs. B, Immunolabeling and confocal microscopy documenting the co-expression of IGF-1R (upper left) and IGF2R (upper central) in freshly sorted hCSCs. Upper right, merge. Sorted hCSCs continue to express IGF-1R (lower left) and IGF-2R (lower right) in culture. C, Phase contrast micrographs of hCSC classes and corresponding PDT values. *,**P<0.05 vs. IGF-1R-positive and IGF-2R-positive hCSCs, respectively. D, BrdU immunolabeling and confocal microscopy of hCSCs and fraction of BrdU-labeled cells. *,**P<0.05 vs. IGF-1R-positive and IGF-2R-positive hCSCs, respectively. E, Euploid set of chromosomes in a metaphase spread of hCSCs at P20. F, Fraction of hCSCs positive for IGF-1R and IGF2-R from P2 to P7. G, BrdU-labeling of hCSC classes in the presence (+) or absence (-) of IGF-1, or IGF-2. *P<0.05 vs. hCSCs in the absence of IGF-1; †P<0.05 vs. IGF-1R-positive hCSCs. H, BrdU-labeling of IGF-1R-positive hCSCs in the presence (+) or absence (-) of IGF-1, or IGF-2, and in the presence or absence of IGF-1R neutralizing antibody. *,**,***^,†P<0.05 vs. unsorted hCSCs, absence of IGF-1, absence of IGF-1R antibody, absence of IGF-2, respectively. I, Expression of phospho-Akt^Ser473 in IGF-1R-positive IGF-2R-negative hCSCs stimulated with IGF-1 or IGF-2 for 10 and 60 minutes. Control, untreated hCSCs. GAPDH, loading conditions. OD, optical density. J, Nuclear localization of phospho-Akt^Ser473 in IGF-1R-positive IGF-2R-negative hCSCs stimulated with IGF-1 or IGF-2 for 10 and 60 minutes.