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Nat Chem Biol. Author manuscript; available in PMC 2009 Jun 1.
Published in final edited form as:
Nat Chem Biol. 2008 Dec; 4(12): 742–750.
doi: 10.1038/nchembio.124
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Figure 2

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Human whole blood coagulated on spatially localized B. cereus in the presence of flow. (a) A microphotograph shows the microfluidic device used to flow human whole blood over colonies of bacteria. The bacteria were localized in GMDs that also contained magnetic particles. Magnets were used to localize the GMDs in the device. (b) A brightfield image shows coagulation of human whole blood on GMDs containing B. cereus expressing GFP (overlaid green fluorescence). (c,d) Microphotographs of fluorescence show coagulation of human whole blood (blue) on GMDs containing B. cereus (green) (c) but not on E. coli (green) (d). White dashed lines outline the channel walls. Images were taken 11 min after blood was introduced into the device. (e) A graph shows the clot times of flowing whole blood on colonies of B. cereus and E. coli in microfluidic devices. Each data point represents an independent experiment.

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