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Appl Environ Microbiol. 1991 Feb; 57(2): 463–468.
PMCID: PMC182733

Lipid Content and Cryotolerance of Bakers' Yeast in Frozen Doughs †

Pierre Gélinas,1,2,‡* Gisèle Fiset,2 Claude Willemot,3 and Jacques Goulet2
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Abstract

The relationship between lipid content and tolerance to freezing at −50°C was studied in Saccharomyces cerevisiae grown under batch or fed-batch mode and various aeration and temperature conditions. A higher free-sterol-to-phospholipid ratio as well as higher free sterol and phospholipid contents correlated with the superior cryoresistance in dough or in water of the fed-batch-grown compared with the batch-grown cells. For both growth modes, the presence of excess dissolved oxygen in the culture medium greatly improved yeast cryoresistance and trehalose content (P. Gélinas, G. Fiset, A. LeDuy, and J. Goulet, Appl. Environ. Microbiol. 26:2453-2459, 1989) without significantly changing the lipid profile. Under the batch or fed-batch modes, no correlation was found between the cryotolerance of bakers' yeast and the total cellular lipid content, the total sterol content, the phospholipid unsaturation index, the phosphate or crude protein content, or the yeast cell morphology (volume and roundness).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Allen RJ. The estimation of phosphorus. Biochem J. 1940 Jun;34(6):858–865. [PMC free article] [PubMed]
  • BLIGH EG, DYER WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. [PubMed]
  • Brown CM, Johnson B. Influence of oxygen tension on the physiology of Saccharomyces cerevisiae in continuous culture. Antonie Van Leeuwenhoek. 1971;37(4):477–487. [PubMed]
  • Calcott PH, MacLeod RA. Survival of Escherichia coli from freeze-thaw damage: influence of nutritional status and growth rate. Can J Microbiol. 1974 May;20(5):683–689. [PubMed]
  • FOLCH J, LEES M, SLOANE STANLEY GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed]
  • Gélinas P, Fiset G, Leduy A, Goulet J. Effect of growth conditions and trehalose content on cryotolerance of bakers' yeast in frozen doughs. Appl Environ Microbiol. 1989 Oct;55(10):2453–2459. [PMC free article] [PubMed]
  • Goldberg I, Eschar L. Stability of lactic Acid bacteria to freezing as related to their Fatty Acid composition. Appl Environ Microbiol. 1977 Mar;33(3):489–496. [PMC free article] [PubMed]
  • Hunter K, Rose AH. Lipid composition of Saccharomyces cerevisiae as influenced by growth temperature. Biochim Biophys Acta. 1972 Apr 18;260(4):639–653. [PubMed]
  • Kaneko H, Hosohara M, Tanaka M, Itoh T. Lipid composition of 30 species of yeast. Lipids. 1976 Dec;11(12):837–844. [PubMed]
  • KATES M, BAXTER RM. Lipid composition of mesophilic and psychrophilic yeasts (Candida species) as influenced by environmental temperature. Can J Biochem Physiol. 1962 Sep;40:1213–1227. [PubMed]
  • McKersie BD, Lepock JR, Kruuv J, Thompson JE. The effects of cotyledon senescence on the composition and physical properties of membrane lipid. Biochim Biophys Acta. 1978 Apr 4;508(2):197–212. [PubMed]
  • MORRISON WR, SMITH LM. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed]
  • Parks LW, Bottema CD, Rodriguez RJ, Lewis TA. Yeast sterols: yeast mutants as tools for the study of sterol metabolism. Methods Enzymol. 1985;111:333–346. [PubMed]
  • Souzu H. Fluorescence polarization studies on Escherichia coli membrane stability and its relation to the resistance of the cell to freeze-thawing. I. Membrane stability in cells of differing growth phase. Biochim Biophys Acta. 1986 Oct 9;861(2):353–360. [PubMed]
  • Taylor FR, Parks LW. Metabolic interconversion of free sterols and steryl esters in Saccharomyces cerevisiae. J Bacteriol. 1978 Nov;136(2):531–537. [PMC free article] [PubMed]
  • Tyson CB, Smith QJ, Wheals AE. Mean size of Saccharomyces cerevisiae cells growing at different growth rates [proceedings]. Biochem Soc Trans. 1978;6(3):564–565. [PubMed]
  • Uemura M, Yoshida S. Involvement of Plasma Membrane Alterations in Cold Acclimation of Winter Rye Seedlings (Secale cereale L. cv Puma). Plant Physiol. 1984 Jul;75(3):818–826. [PMC free article] [PubMed]
  • Vraná D. Daughter cells as an important factor in determining the physiological state of yeast populations. Biotechnol Bioeng. 1976 Mar;18(3):297–309. [PubMed]
  • Walker GM, Duffus JH. Magnesium ions and the control of the cell cycle in yeast. J Cell Sci. 1980 Apr;42:329–356. [PubMed]
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