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Environ Health Perspect. 1996 Mar; 104(3): 298–305.
doi: 10.1289/ehp.96104298
PMCID: PMC1469315
PMID: 8919768
Research Article

Estrogenicity of resin-based composites and sealants used in dentistry.

N Olea, R Pulgar, P Pérez, F Olea-Serrano, A Rivas, A Novillo-Fertrell, V Pedraza, A M Soto, and C Sonnenschein
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Abstract

We tested some resin-based composites used in dentistry for their estrogenic activity. A sealant based on bisphenol-A diglycidylether methacrylate (bis-GMA) increased cell yields, progesterone receptor expression, and pS2 secretion in human estrogen-target, serum-sensitive MCF7 breast cancer cells. Estrogenicity was due to bisphenol-A and bisphenol-A dimethacrylate, monomers found in the base paste of the dental sealant and identified by mass spectrometry. Samples of saliva from 18 subjects treated with 50 mg of a bis-GMA-based sealant applied on their molars were collected 1 hr before and after treatment. Bisphenol-A (range 90-931 micrograms) was identified only in saliva collected during a 1-hr period after treatment. The use of bis-GMA-based resins in dentistry, and particularly the use of sealants in children, appears to contribute to human exposure to xenoestrogens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  • Sharpe RM, Skakkebaek NE. Are oestrogens involved in falling sperm counts and disorders of the male reproductive tract? Lancet. 1993 May 29;341(8857):1392–1395. [PubMed] [Google Scholar]
  • Wolff MS, Toniolo PG, Lee EW, Rivera M, Dubin N. Blood levels of organochlorine residues and risk of breast cancer. J Natl Cancer Inst. 1993 Apr 21;85(8):648–652. [PubMed] [Google Scholar]
  • Krishnan AV, Stathis P, Permuth SF, Tokes L, Feldman D. Bisphenol-A: an estrogenic substance is released from polycarbonate flasks during autoclaving. Endocrinology. 1993 Jun;132(6):2279–2286. [PubMed] [Google Scholar]
  • Brotons JA, Olea-Serrano MF, Villalobos M, Pedraza V, Olea N. Xenoestrogens released from lacquer coatings in food cans. Environ Health Perspect. 1995 Jun;103(6):608–612. [PMC free article] [PubMed] [Google Scholar]
  • Hanks CT, Craig RG, Diehl ML, Pashley DH. Cytotoxicity of dental composites and other materials in a new in vitro device. J Oral Pathol. 1988 Sep;17(8):396–403. [PubMed] [Google Scholar]
  • Hensten-Pettersen A, Helgeland K. Sensitivity of different human cell line in the biologic evaluation of dental resin-based restorative materials. Scand J Dent Res. 1981 Feb;89(1):102–107. [PubMed] [Google Scholar]
  • Terakado M, Yamazaki M, Tsujimoto Y, Kawashima T, Nagashima K, Ogawa J, Fujita Y, Sugiya H, Sakai T, Furuyama S. Lipid peroxidation as a possible cause of benzoyl peroxide toxicity in rabbit dental pulp--a microsomal lipid peroxidation in vitro. J Dent Res. 1984 Jun;63(6):901–905. [PubMed] [Google Scholar]
  • Hanks CT, Strawn SE, Wataha JC, Craig RG. Cytotoxic effects of resin components on cultured mammalian fibroblasts. J Dent Res. 1991 Nov;70(11):1450–1455. [PubMed] [Google Scholar]
  • BOURNE LB, MILNER FJ, ALBERMAN KB. Health problems of epoxy resins and amine-curing agents. Br J Ind Med. 1959 Apr;16(2):81–97. [PMC free article] [PubMed] [Google Scholar]
  • Morrissey RE, George JD, Price CJ, Tyl RW, Marr MC, Kimmel CA. The developmental toxicity of bisphenol A in rats and mice. Fundam Appl Toxicol. 1987 May;8(4):571–582. [PubMed] [Google Scholar]
  • Bentley P, Bieri F, Kuster H, Muakkassah-Kelly S, Sagelsdorff P, Stäubli W, Waechter F. Hydrolysis of bisphenol A diglycidylether by epoxide hydrolases in cytosolic and microsomal fractions of mouse liver and skin: inhibition by bis epoxycyclopentylether and the effects upon the covalent binding to mouse skin DNA. Carcinogenesis. 1989 Feb;10(2):321–327. [PubMed] [Google Scholar]
  • Steiner S, Hönger G, Sagelsdorff P. Molecular dosimetry of DNA adducts in C3H mice treated with bisphenol A diglycidylether. Carcinogenesis. 1992 Jun;13(6):969–972. [PubMed] [Google Scholar]
  • Ferracane JL, Condon JR. Rate of elution of leachable components from composite. Dent Mater. 1990 Oct;6(4):282–287. [PubMed] [Google Scholar]
  • Freund M, Munksgaard EC. Enzymatic degradation of BISGMA/TEGDMA-polymers causing decreased microhardness and greater wear in vitro. Scand J Dent Res. 1990 Aug;98(4):351–355. [PubMed] [Google Scholar]
  • Ripa LW. Sealants revisted: an update of the effectiveness of pit-and-fissure sealants. Caries Res. 1993;27 (Suppl 1):77–82. [PubMed] [Google Scholar]
  • Climie IJ, Hutson DH, Stoydin G. Metabolism of the epoxy resin component 2,2-bis[4](2,3]epoxypropoxy)phenyl]propane, the diglycidyl ether of bisphenol A (DGEBPA) in the mouse. Part I. A comparison of the fate of a single dermal application and of a single oral dose of 14C-DGEBPA. Xenobiotica. 1981 Jun;11(6):391–399. [PubMed] [Google Scholar]
  • Climie IJ, Hutson DH, Stoydin G. Metabolism of the epoxy resin component 2,2-bis[4-(2,3-epoxypropoxy)phenyl]propane, the diglycidyl ether of bisphenol A (DGEBPA) in the mouse. Part II. Identification of metabolites in urine and faeces following a single oral dose of 14C-DGEBPA. Xenobiotica. 1981 Jun;11(6):401–424. [PubMed] [Google Scholar]
  • Soule HD, Vazguez J, Long A, Albert S, Brennan M. A human cell line from a pleural effusion derived from a breast carcinoma. J Natl Cancer Inst. 1973 Nov;51(5):1409–1416. [PubMed] [Google Scholar]
  • Soto AM, Sonnenschein C. The role of estrogens on the proliferation of human breast tumor cells (MCF-7). J Steroid Biochem. 1985 Jul;23(1):87–94. [PubMed] [Google Scholar]
  • Villalobos M, Olea N, Brotons JA, Olea-Serrano MF, Ruiz de Almodovar JM, Pedraza V. The E-screen assay: a comparison of different MCF7 cell stocks. Environ Health Perspect. 1995 Sep;103(9):844–850. [PMC free article] [PubMed] [Google Scholar]
  • Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, Vistica D, Warren JT, Bokesch H, Kenney S, Boyd MR. New colorimetric cytotoxicity assay for anticancer-drug screening. J Natl Cancer Inst. 1990 Jul 4;82(13):1107–1112. [PubMed] [Google Scholar]
  • Madeddu L, Legros N, Devleeschouwer N, Bosman C, Piccart MJ, LeClercq G. Estrogen receptor status and estradiol sensitivity of MCF-7 cells in exponential growth phase. Eur J Cancer Clin Oncol. 1988 Mar;24(3):385–390. [PubMed] [Google Scholar]
  • Vankerckhoven H, Lambrechts P, van Beylen M, Vanherle G. Characterization of composite resins by NMR and TEM. J Dent Res. 1981 Dec;60(12):1957–1965. [PubMed] [Google Scholar]
  • Rueggeberg FA, Craig RG. Correlation of parameters used to estimate monomer conversion in a light-cured composite. J Dent Res. 1988 Jun;67(6):932–937. [PubMed] [Google Scholar]
  • Lobos JH, Leib TK, Su TM. Biodegradation of bisphenol A and other bisphenols by a gram-negative aerobic bacterium. Appl Environ Microbiol. 1992 Jun;58(6):1823–1831. [PMC free article] [PubMed] [Google Scholar]
  • Maguire HC., Jr Experimental photoallergic contact dermatitis to bisphenol A. Acta Derm Venereol. 1988;68(5):408–412. [PubMed] [Google Scholar]
  • Stanley HR, Bowen RL, Folio J. Compatibility of various materials with oral tissues. II: Pulp responses to composite ingredients. J Dent Res. 1979 May;58(5):1507–1517. [PubMed] [Google Scholar]
  • Inoue K, Hayashi I. Residual monomer (Bis-GMA) of composite resins. J Oral Rehabil. 1982 Nov;9(6):493–497. [PubMed] [Google Scholar]
  • Weintraub JA. The effectiveness of pit and fissure sealants. J Public Health Dent. 1989;49(5 Spec No):317–330. [PubMed] [Google Scholar]
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