Sde Proteins Coordinate Ubiquitin Utilization and Phosphoribosylation to Promote Establishment and Maintenance of the Legionella Replication Vacuole

The Legionella pneumophilaSde family of translocated proteins promote host tubular endoplasmic reticulum (ER) rearrangements that are tightly linked to phosphoribosyl-ubiquitin (pR-Ub) modification of Reticulon 4 (Rtn4). Sde proteins have two additional activities of unclear relevance to the infection process: K63 linkage-specific deubiquitination and phosphoribosyl modification of polyubiquitin (pR-Ub). We show here that the deubiquitination activity (DUB) stimulates ER rearrangements while pR-Ub protects the replication vacuole from cytosolic surveillance by autophagy. Loss of DUB activity was tightly linked to lowered pR-Ub modification of Rtn4, consistent with the DUB activity fueling the production of pR-Ub-Rtn4. In parallel, phosphoribosyl modification of polyUb, in a region of the protein known as the isoleucine patch, caused an absolute block in binding by the autophagy adapter p62. An inability of Sde mutants to modify polyUb resulted in immediate p62 association, a critical precursor to autophagic attack. The ability of Sde WT to block p62 association decayed quickly after bacterial infection, as predicted by the presence of previously characterized L. pneumophila effectors that inactivate Sde and remove polyUb. In sum, these results show that the accessory Sde activities act to stimulate ER rearrangements and protect from host innate immune sensing in a temporal fashion.


Introduction
Legionella pneumophila is a facultative intracellular bacterium that is the causative agent of Legionnaire's disease, a pneumonia of high lethality that primarily occurs in the immunocompromised and individuals with depressed lung function 1,2 .Disease is usually associated with aspiration of droplets from contaminated water sources harboring amoebae that act as the environmental intracellular reservoir to support growth of the bacterium 3 .Once deposited within the lungs, productive infection involves growth of L. pneumophila within alveolar macrophages followed by attempted neutrophil clearing, which accumulates during productive pneumonic disease 4 .The ability of the bacterium to grow within macrophages is tightly linked to an environmental lifestyle that involves growth within amoebae, as establishing a niche within amoebal species provides the selective pressure for acquisition of genes that promote intracellular growth 5,6 .Therefore, human disease promoted by the bacterium is a consequence of targeting host cell functions that are largely conserved from amoebae to man.
Intracellular growth of L. pneumophila requires construction of the Legionella-containing vacuole (LCV), which is derived from host membranes and is tightly associated with host endoplasmic reticulum and the early secretory apparatus 7,8 .The LCV avoids interaction with the endocytic pathway including the highly antimicrobial lysosome 9 .Biogenesis of the bacterial compartment requires the function of the Icm/Dot type IV secretion system that acts as a syringe to deposit translocated bacterial effector proteins into the surrounding membrane as well as into the cytosol 10,11 .Every Legionella species member encodes at least 300 of these effectors, which are remarkably poorly conserved, with only seven proteins found in all species 12,13 .The biochemical activities of a large number of these proteins have been identi ed, many of which perform posttranslational modi cations (PTMs) on host proteins, conferring activation, inhibition, altered regulation or acquisition of new functions on the target proteins 14 .The most notable consequences of these activities are to maintain the integrity of the LCV 15 , promote association with proteins involved in endoplasmic reticulum dynamics 16 , inactivate host components that drive association with the endocytic compartment 17 , and inhibit protein synthesis 18 .
One set of Icm/Dot system translocated proteins that drives a unique morphological transformation of the LCV is the Sde family (SidE, SdeA, SdeB and SdeC) 19,20,21,22 .The importance of this family is emphasized by the discovery that at least ve other L. pneumophila translocated substrates regulate the dynamics of this family 23,24,25,26,27,28 .As is true of many Icm/Dot substrates, each member is a composite of three enzymatic activities located on separate domains (Fig. 1A) 29,30,31 .A mono-ADPribosyltransferase (mART) domain drives NAD-dependent ADPribosylation of diverse ubiquitin species at Arginine 42 (ADPr-Ub) 20,22 .In turn, a phosphodiesterase-like domain (PDE) can use ADPr-Ub to either promote phosphoribosyl linkage of Ub (pR-Ub) to substrates, or hydrolysis to generate free phosphoribosylated-Ub 21,22 .The DUB domain is an accessory activity that preferentially deubiquitinates Lys63-linked polyubiquitin chains 32 .A number of potential mammalian substrates have been shown to be pR-Ub modi ed by Sde members on serine residues that are located in relatively unstructured regions of proteins 29,30 .Work with model substrates indicates that tyrosine residues may also be targeted 33 .
Although the number of Sde substrates is likely to be quite large given the structural preference for pR-Ub modi cation, modi cations of endoplasmic reticulum protein Rtn4 22 and Golgi-associated GRASP family members appear most closely tied to events involved in LCV biogenesis 27,34 .In the absence of DUB activity, there is accumulation of polyUb about the replication vacuole, with unknown consequences on replication vacuole formation or intracellular growth 32 .
Immediately after L. pneumophila is internalized by macrophages, pR-Ub modi cation of Rtn4 drives replication vacuole association with endoplasmic reticulum tubules.This modi cation results in highly detergent-resistant structures that appear to wall off the LCV from the host cell cytosol 22 , in a process that contributes to the protection of the LCV from attack by early endosomal components that degrade the vacuole 35 .Simultaneously, there is accumulation of polyubiquitinated targets about the LCV 36,37,38,39,40 , which appear to be targets of the Sde DUB domain 32,41 .Phosphoribosyl modi cation of polyUb by Sde proteins stabilizes these structures, by blocking wholesale reversal of polyubiquitination by both bacterial and host DUBs 42 .Accumulation of polyUb potentially provides a target for the host cell autophagy pathway that could drive degradation of the LCV 43,44,45 .L. pneumophila appears to have several systems for blocking autophagic recognition of the LCV, including degradation of central components of the autophagy machinery by the Icm/Dot effector RavZ 46 , degradation of syntaxin 17 by effector Lpg1137 47 , as well as the described phosphoribosylation 48 .
In this study, we report the role of the DUB and phosphoribosylation accessory activities of Sde family members.We show that the DUB domain surprisingly increases the e ciency of Rtn4 mobilization about the LCV, while ADPribosylation or phosphoribosylation of Ub interferes with autophagy by causing an absolute physical block on binding of polyUb to an autophagy adaptor, primarily by phosphoribosylation of target Ub chains about the LCV.Similar results have been obtained in the simultaneously submitted manuscript by Wan, et al. 49

Results
The Sde DUB activity is required for e cient Rtn4 rearrangements in response to L. pneumophila.
To determine if there is a link between the Sde family K63 deubiquitinase (DUB) domain (Fig. 1A) and biogenesis of the Legionella-containing vacuole (LCV), the kinetics of polyubiquitin (polyUb) association and Rtn4 rearrangements about the LCVs were analyzed.A previous study had shown that the absence of the DUB domain resulted in a 2-fold increase in polyUb about the LCV 32 .Bone marrow-derived macrophages (BMDMs) were challenged with L. pneumophila derivatives lacking either the complete complement of sde family members (sidE, sdeABC) or missing sdeABC (ΔsdeC-A), which removes all proteins known to control Rtn4 dynamics 19 .After 5 min centrifugation onto BMDMs, followed by xation and probing with a polyUb-speci c antibody (FK1), there was no evidence of polyUb about the LCV of WT strains (Fig. 1B; 0 minutes post infection (MPI)).In contrast, challenge with L. pneumophila missing all sde family members (Δsde) resulted in over 30% of the LCVs displaying polyUb association.By 10 MPI, ~ 15% of the vacuoles containing WT strain positively stained with polyUb, approximately 4X lower than the Δsde strain at this timepoint, consistent with previous observations (Fig. 1B; 32 ).At 0 MPI, Δsde mutants were e ciently complemented by all three members of the Sde family known to have biological activity (SdeA, SdeB and SdeC; Fig. 1B), although complementation of ΔsdeC-A mutants retaining SidE was weaker (Fig. 1C).Surprisingly, for all complemented strains, polyUb quickly rose to the levels seen in the absence of Sde family function (Fig. 1B,C; 30 MPI).
The ability to prevent polyUb association with the LCV at the earliest timepoint was dependent on the activity of the DUB domain, as the SdeC-C118S active site mutation was unable to block polyUb association at the earliest timepoint (Fig. 1C; 0 MPI).We conclude that inhibition of the polyUb association with the LCV occurs transiently and is dependent on the Sde deubiquitinase activity.The fact that the inhibition is transient is consistent with the LCV-associated polyUb chains becoming resistant to the action of the DUB domain by 30 minutes post-infection (MPI).
The biological signi cance of having a K63 deubiquitinase activity is unclear, especially because the ART activity of Sde proteins directly blocks deubiquitination, arguing that the DUB may play some role other than reducing LCV polyubiquitination 22,42 .Alternatively, the DUB activity could modulate the dynamics of Rtn4 rearrangements about the LCV 22 .To test this model, BMDMs were challenged with L. pneumophila derivatives having defective DUB activity followed by probing for Rtn4 detergent-resistant structures about the LCV (Figs. 1D,E) 22 .Surprisingly, the inactive DUB mutant (C118S) reduced Rtn4 colocalization with the LCV.In strains expressing either SdeA, SdeB or SdeC as the sole demonstrated drivers of Rtn4 rearrangement, the C118S mutation reduced detergent-resistant Rtn4 association with the LCV by approximately 50% (Figs.1D,E).Plasmid-borne SdeA showed lowered complementation levels (Figs.1D,E), but SdeA also drove detergent-resistant Rtn4 structures that were not LCV-associated, indicating that SdeA overproduction may result in leakage of the protein from the LCV, as has been documented previously 19 .
Consistent with data in Fig. 1E, Rtn4 association with the LCV was robust in strains expressing SdeB or SdeC as the sole plasmid-expressed isoforms (Figs.1I,J) and indistinguishable from the WT strain (Fig. 1F).In contrast, loss of sdeC-A prevented Rtn4 association with the LCV, as did elimination of the T4SS (Figs. 1G, H).The presence of the C118S mutation resulted in two populations, with a mixture of robust Rtn4-colocalization with LCVs or RTN4 staining below the level of detection (Fig. 1K).Similar results were seen with the DUB D80A catalytic mutant, whereas a control Cys mutant C293S showed high levels of colocalization (Fig. S1).These results are consistent with the DUB domain playing a role in LCV biogenesis at the level of controlling Rtn4 dynamics.
To test if the Sde DUB activity plays a direct role in promoting Rtn4 rearrangements, we determined if the catalytic domain contributed to the rate of Sde-dependent phosphoribosyl-ubiquitin (pR-Ub) modi cation of Rtn4.In a puri ed system, pR-Ub modi cation of Rtn4 is rapid and robust with the presence of excess mono-Ub 22,33 .In contrast, infection provides multiple potential sources of Ub, including K63-linked polyUb, which the DUB activity could target to provide substrate for pR-Ub modi cation.To test this hypothesis, HEK293T cells transfected with HA-Ub were challenged by L. pneumophila harboring either WT or DUB-defective (C118S) derivatives as the only active Sde family members.At 10 or 180 MPI, cells were extracted and subjected to immunoprecipitation with anti-Rtn4, followed by immunoprobing with anti-HA to reveal Rtn4 ubiquitination (Fig. 1L).Detectable mono-and di-modi cation of the Rtn4B and Rtn4B2 isoforms were observed as early as 10 MPI with continued increase at 180 MPI in response to the WT strain (Fig. 1L, WT).In contrast, challenge with Δsde mutants showed HA-Ub modi cation that was indistinguishable from mock-infected controls (Fig. 1M; vector vs. Mock).Challenge with Δsde mutants harboring single SdeA or SdeB isoforms each showed rescue of the HA-Ub modi cation defect, and their rescue was largely eliminated by the presence of the C118S catalytic mutation (Fig. 1M; compare psdeA and psdeB to the respective C118S derivatives).Therefore, the absence of Sde DUB activity results in a strong reduction in both detergent-resistant Rtn4 and Ub modi cation of Rtn4, strongly arguing that Sdemediated deubiquitination of K63-linked polyUb plays a role in LCV dynamics, possibly providing a pool of mono-Ub substrate for the ART domain.
ADPribosylation of ubiquitin by Sde results in hyper-ubiquitination of the LCV.
The data that polyUb association with the LCV occurs robustly immediately after bacterial contact in the absence of the DUB domain argues that the likely role of pR-modi cation is to stabilize polyUb (Fig. 1).Furthermore, both pR-modi cation and ADP-ribosylation by Sde block bacterial DUBs as well as a variety of host-encoded K63-and K48-speci c DUBs (Fig. 2A) 42 , arguing that pR modi cation of Ub does not play a signi cant role in blocking polyubiquitination during LCV formation as proposed previously 21 .
The L. pneumophila sdeC H416A (PDE − ) mutant results in ADPr modi cation of Ub, preventing pRubiquitination of target proteins or the generation of pR-Ub.The consequent ADPr-modi ed polyubiquitinated structures are resistant to DUB attack (Fig. 2A), allowing interrogation of Ub modi cation without confounding Rtn4 rearrangements.To analyze the consequences of L. pneumophila derivatives altered in Sde family function, BMDM monolayers were challenged for 20 min, and changes in polyUb about the LCV were analyzed by probing xed samples with a polyUb-speci c antibody (Fig. 2B).
In contrast, challenge with the L. pneumophila PDE − mutant resulted in dense accumulation of LCVassociated polyubiquitination in spite of the fact that Rtn4 rearrangements were blocked (Fig. 2B).This dense association was maintained over time (Fig. 2C).Most notable was the presence of unusual polyUbmodi ed structures after challenge with the PDE − mutant.While the Δsde and ART − mutants showed polyUb staining about individual LCVs (Figs. 2E,G), the PDE − mutant showed polyUb structures that wrapped around the LCV and extended into serpentine structures across the interior of the BMDM (Fig. 2H).We occasionally observed dense structures in response to challenge with the DUB − mutant as well (Fig. 2F), but the frequency was little changed from WT (Figs. 2B,C).We conclude that Sde-mediated modi cation of polyUb chains stabilizes LCV-associated Ub from the attack by both bacterial-and host derived DUBs.The observed deregulation of associated polyubiquitination in the absence of PDE activity indicates that direct modi cation of Ub is su cient to stabilize polyUb.Furthermore, there is no evidence that pR-or ADPr-Ub modi cation plays a role in blocking polyubiquitination.
The Ub Arg42 residue is a key target for modulating ubiquitin dynamics.Autophagy adaptors such as p62 link ubiquitinated compartments to LC3-decorated phagophores via ubiquitin associated (UBA) domains that recognize a hydrophobic patch on the Ub surface 50,51 .Integral to this patch are the residues I44, V70 and L71, directly proximal to R42, the target residue of ADPr modi cation by Sde (Fig. 3A, blue arrows).Structural analysis has shown that R42 can directly participate in the UBA interface (Fig. 3B) and is likely involved in p62 recognition based on NMR analysis 52,53 .We therefore tested if modi cation of the Ub at Arg42 residue blocks recognition by the p62 autophagy adaptor.
To determine the effects of Ub Arg42 modi cation in a more quantitative assay, binding a nities to Ub 4 were determined using surface plasmon resonance (SPR) in the presence of a DUB inhibitor (Ubpropargylamide, Ub-PA) to prevent degradation of the Ub 4 polymer (Fig. S2A).K63-linked Ub 4 was modi ed by SdeC WT or its catalytic mutant derivatives in the presence of Ub-PA (Materials and Methods), the modi ed K63-linked Ub 4 was introduced onto ow chips with immobilized p62, and binding was measured by SPR over time (Fig. 3E).In the absence of SdeC, Ub 4 bound His-p62 with apparent K D ~1 X 10 − 7 M, indicating that binding a nity was at least as e cient as previous SPR studies using diUb as a substrate (K D = 9.3 X 10 − 8 M; 54 ) (Fig. 3F).Binding of p62 to mono-Ub was very poor, consistent with DUB activity interfering with recognition of p62 (Fig. 3G, Fig. S2C).Ub 4 pretreated with SdeC, either in the presence of Ub-PA or having the C118S DUB mutation, resulted in blockade of p62 binding, consistent with phosphoribosylation blocking recognition of Ub by UBA domains (Figs. 3H,I).ADPr modi cation of Ub at Arg42 was su cient to block binding, as the SdeC H416A PDE − mutant blocked binding as e ciently as WT (Fig. 3J).Elimination of the ART activity restored Ub 4 binding to p62, with a similar binding a nity to untreated, consistent with PA blocking the DUB activity (Fig. 3K; K D = 1.6 X 10 − 7 M).These results strongly argue that modi cation of the Ub at Arg42 residue by SdeC disrupts recognition by p62.
Sde-mediated modi cation of polyUb chains prevents association of p62 with the LCV.
To determine if Sde proteins enzymatically camou age the replication vacuole from recognition by p62, BMDMs were challenged with L. pneumophila sde variants and probed for p62 localization about the LCV.A variety of p62 morphological variants were observed.After bacterial contact (5 min centrifugation) and immediate xation, LCVs harboring L. pneumophila WT were free from the punctate localization of p62 found throughout the cytoplasm (Fig. 4A).In contrast, a large fraction of the vacuoles after challenge with the L. pneumophila Δsde strain were enveloped by p62 (Fig. 4B).As time progressed, a variety of morphologies became apparent, such as the alignment of puncta proximal to the LCV.As puncta associated with the LCV were di cult to distinguish from those in the general vicinity, we limited quanti cation to identifying vacuoles with enveloping p62 structures and determining total p62 intensity associated with individual vacuoles.
Immediately after bacterial contact with BMDMs, both the WT and the T4SS − strains were devoid of p62 staining (Fig. 4C).In contrast, approximately 30% of the L. pneumophila Δsde bacteria were found to be enveloped by p62 (Fig. 4C).Colocalization was largely lost when either sdeA, sdeB, or sdeC expressed on plasmids was introduced into the Δsde strain.Quanti cation of p62 signal associated with the LCV showed that there were two populations of bacteria after challenge with the Δsde strain, with a population showing p62 recruitment levels that were similar to WT and a second population with 8-10X higher uorescence intensity (Fig. 4D).In contrast, the WT and Δsde strains harboring Sde isoforms in trans showed indistinguishable low levels of p62 recruitment (Fig. 4D).
As the infection proceeded, colocalization of p62 with the Δsde strain continued to increase (Fig. 4E, > 50% at 20 MPI) with the population of LCVs strongly skewing toward hyperaccumulation of p62 (Fig. 4F).Concurrently, a second phenomenon became apparent in strains harboring plasmids that produced excess Sde isoforms.There was clear complementation of the Δsde defect (Fig. 4E), but accumulation of p62 about the LCV at 20 MPI was observed that distinguished these constructs from the WT strain (Fig. 4E).Accumulation could also be observed when the uorescence intensity of individual LCVs was determined, with a population emerging with increased p62 density (Fig. 4F).This phenomenon appeared to be a premature version of what occurs with the WT strain, as 20% of the LCVs harboring the WT showed accumulation of p62 by 60 MPI, with evidence of an emerging population showing increased p62 density about the LCV (Figs. 4G, H).There was robust accumulation of p62 about individual LCVs harboring WT strain at this timepoint that appeared indistinguishable from individual LCVs harboring the Δsde strain (Fig. 4I).Furthermore, at 180MPI, accumulation of p62 around the LCV in the Δsde strain was indistinguishable from p62 accumulation around WT LCVs (Figs. 4J, K).When the percentage of LCVs associated with p62 was plotted as a function of time post-infection (Fig. 4L), there was early accumulation of p62 that was blocked by the Sde family (0-20 MPI).This was followed by decay of p62 accumulation after infection of the Δsde strain, while the WT showed increasing p62 between 20 and 60 MPI, resulting in a convergence of the phenotypes (180 MPI).The loss of Sde protection of the LCV is consistent with reversal of its activity by known metaeffectors SidJ and DupA/B 24,25,26,55 .
To determine the role of the individual Sde activities in preventing p62 accumulation, point mutations in each of the three catalytic domains were analyzed at 0 MPI after macrophage challenge, when the Sde family exerts its most striking effects.Mutations in sdeB were introduced into the Δsde strain, as this isoform showed the most effective complementation (Fig. 4C), and these mutants were then used to challenge macrophages.Point mutations in the catalytic sites of the DUB or PDE domains, as well as a double mutant missing the catalytic activity in both of these domains, still retained the ability to block p62 colocalization after association (Figs.5A-C).In contrast, a mutation in the ART domain caused a large increase in p62 recruitment to LCVs, indicating that ADP ribosylation of Ub Arg42 by the ART domain blocks p62 recognition of the LCVs (Fig. 5A).It should be noted that there appeared to be some residual interference of p62 in the single ART mutant, but this was eliminated by simultaneously introducing a DUB mutation, indicating that the DUB domain may play a minor role in lowering p62 recognition of the LCV (Fig. 5A).As seen with the Δsde strain, the ART mutants resulted in a clear subpopulation showing approximately 10-fold increase in p62 accumulations (Figs.5B,C).These results support the biochemical data that either ADPribosylation or phosphoribosylation of Ub at Arg42 is su cient to block p62 recognition.It also argues against models that propose pR-linked Ub modi cation of a peripheral factor plays a role 56 , as ADPribosylation of Ub was su cient to interfere with p62 recruitment.

Discussion
The L. pneumophila Sde family drives phosphoribosyl-linked ubiquitination (pR-Ub) of targets, most spectacularly leading to largescale rearrangements of the tubular ER protein reticulon 4 (Rtn4) 22 .In addition, there are two accessory activities of Sde proteins that have unclear biological roles.The Sde deubiquitinase (DUB) domain preferentially deubiquitinates Lys63-linked polyubiquitin chains (Fig. 1) 32 , while the other is the hydrolytic product of ADPribosylated ubiquitin (ADPr-Ub), resulting in phosphoribosylated ubiquitin (pR-Ub) 22,57 .The phosphoribosylation occurs at the Ub Arg42 residue, strategically placed in a hydrophobic patch that provides a recognition surface for ubiquitin binding proteins that drive autophagy (Fig. 3A,B) 52,58 .The presence of Sde family members is linked to a lowered association of Ub-recognizing autophagy adaptors to the LCVs 48 , consistent with phosphoribosylation promoting maintenance of the LCV in the face of cytosolic antimicrobial attacks.
In this manuscript, we showed that inhibition of polyUb formation about the replication vacuole was transiently controlled by the Sde DUB domain and limited to the earliest times after infection (Fig. 1).This early degradation of polyUb by the DUB domain fueled phosphoribosyl-linked ubiquitination because pR-Ub modi cation of Rtn4 slowed greatly in the absence of DUB activity and was accompanied by a reduction in Rtn4 accumulation about the replication vacuole (Fig. 1D-M).Therefore, it appears that there is K63-polyubiquitination of the LCV about the nascent replication vacuoles 39,59,60,61,62,63 that provides the substrate for the Sde DUB activity, generating monoubiquitin fuel for the Sde mART domain (Fig. 6B,C).This is then directly transferred by the PDE domain to generate pR-Ub modi ed Rtn4, allowing a direct pathway from vacuolar polyUb to endoplasmic reticulum (ER) rearrangements (Fig. 6D).
The association of polyUb with the replication vacuole should be a suicide signal for L. pneumophila, as ubiquitination marks intracellular compartments for degradation by the autophagy pathway 45,64 .Autophagy results from binding of adaptor molecules (such as p62) to Ub-mod ed compartments which, in turn, bridge to autophagophores decorated by LC3/Atg8 65 .It is well established that after L. pneumophila forms the LCV, autophagy is blocked via the action of the Icm/Dot substrate RavZ, which irreversibly hydrolyzes and deconjugates LC3/Atg8 from the nascent autophagophore, blocking binding to autophagy adaptors 46 .In the absence of RavZ, the LCV is still resistant to autophagy, indicating that the vacuole is masked in a fashion that prevents recognition by the LC3-decorated autophagophore 46,48 .We show here that modifying the Arg42 residue of polyUb with either mono-ADPribose or phosphoribose physically blocks binding to the autophagy adaptor p62 (Fig. 3), and similar results have been obtained by Wan and coworkers 49 .Surface plasmon resonance experiments showed no detectable p62 binding in presence of high concentrations of polyUb modi ed by either WT (pR modi cation) or the H416A derivative (ADPr modi cation; Fig. 3F-K).Consistent with the data using puri ed proteins, both modi cations blocked p62 association with the LCV (Fig. 5) and stabilized polyUb about the replication vacuole (Fig. 2).In fact, the ADPr modi cation resulted in hyperaccumulation of polyUb about the replication vacuole (Fig. 2B,C), consistent with enhanced resistance to both DUBs and autophagy adaptor recognition.
That all the proposed functions of phosphoribosylation can be ful lled without hydrolysis of the ADPr modi cation questions the purpose of the pR modi cation.It seems likely that allowing vacuoleassociated polyUb to accumulate ADPr modi cations would result in covalent linkage of Sde targets directly to the replication vacuole, even if this is a low likelihood event 22,27,34 .In addition, the ART and PDE domains work independently and consecutively, allowing ADPr modi cation to persist on polyubiquitin chains, raising the possibility that unwanted proteins would be immobilized about the replication vacuole.This problem is particularly exacerbated by the fact that Sde-promoted pR-Ub linkage preferentially occurs on unstructured regions of proteins, which could drive promiscuous linkage of poorly folded proteins about the vacuole.Therefore, hydrolysis of ADPr-Ub at Arg42 acts as a timing device, requiring target to be immediately accessible to Sde and ADPr-Ub prior to decay to pR-Ub.
It has been previously reported that pR-Ub modi cation of USP14, a host cell deubiquitinase, is a central strategy for preventing p62 association with the Legionella replication vacuole 56 .The authors proposed that modi cation of USP14 disrupted its direct interaction with p62, preventing association of the LCV with the autophagy adaptor 56 .The data we presented here argue against USP14 modi cation being a major mechanism for autophagy avoidance, and that modi cation of LCV-associated Ub is likely the primary strategy used by Sde proteins to protect against autophagy.First, pR-modi cation of Ub blocked p62 association (Fig. 3), and, given the expected stoichiometry of Ub relative to USP14, it is likely that this direct blockade is the primary contributor to autophagy avoidance.Secondly, ADPr-modi cation of poly-Ub was su cient to avoid autophagy (Figs.2B, 5A), arguing that pr-Ub modi cations of any peripheral targets such as USP14 are unlikely to contribute to autophagy avoidance.Finally, we showed that there was a minor, but detectable, contribution of the DUB domain to autophagy avoidance (Fig. 5A), consistent with host DUBs making similar enzymatic contributions, rather than playing a role in direct binding to the p62 target.
Our data on the transient nature of p62 blockade illustrates an important biological principal underlying Sde family function (Fig. 4L).There is little, if any, association of p62 with the L. pneumophila WT replication vacuole during the rst 20 minutes of macrophage infection.In contrast, challenge with the Δsde strain results in p62 association with the LCV at early timepoints (Figs.4E,L).This huge difference largely disappears by 1 hour post infection, as there appears to be increased accumulation of p62 about the WT LCV, with coordinate decreased p62 accumulation about the Δsde LCV (Fig. 4L).By 3 hr post infection, the Δsde mutant and WT look indistinguishable, with each showing about 80% of the LCVs devoid of p62 localization.Therefore, function of Sde decays rapidly during the rst hour after infection, with a new activity appearing in both strain backgrounds that interferes with p62 association.
The decay in protection from p62 association is predicted by the function of proteins that modulate Sde family activity.Icm/Dot translocated proteins SidJ and SdjA glutamylate Sde proteins, blocking their ability to ADPribosylate ubiquitin 23,24,25,26 .As expression of the sde genes appears to be downregulated after contact with mammalian cells 19,66 , this modi cation effectively removes the pool of enzyme capable of ADPribosylating Ub.The fact that over time there is some increase in the level of p62 association with the WT LCV indicates that there is accumulation of polyubiquitin about the LCV during the rst hour after infection that cannot be modi ed by the inactivated Sde enzymes.In this regard, it should be noted that pR-Ub modi cation of target proteins is also reversed by the linkage-speci c deubiquitinases DupA and DupB, consistent with a general decay in Sde function as well as loss of memory of its activity during the rst hour after infection 27,28 .Therefore, Sde functions as critical cog in the morphogenesis of the LCV during the earliest stages of replication vacuole establishment.E cient intracellular growth requires decay in Sde function, as SidJ mutants that result in extended Sde family activity show slowed intracellular growth 67 .
In summation, the Sde family is involved in critical early steps that are associated with homeostatic regulation of replication vacuole morphogenesis.Accessory activities that modulate polyubiquitin dynamics about the replication vacuole are tightly linked to phosphoribosyl-linked ubiquitination of substrate (Fig. 6).We argue that the DUB domain provides fuel for pR-Ub modi cation, while pR modi cation of polyubiquitination about the LCV prevents attack by an important arm of host cell cytosolic defense.Finally, the transient nature of Sde function during replication vacuole formation is an intrinsic feature of this system, as we witness decay in the ability to block the autophagic response.The transient nature of providing fuel for Sde function is also supported by this work, as phosphoribosyl modi cation of polyUb is predicted to block DUB function (Fig. 2) and reduce the amount of fuel to support Sde-mediated ubiquitination of substrates.The biological importance of limiting Sde function to the earliest stages of replication compartment biogenesis requires further investigation.
For the infection of mammalian cells, L. pneumophila strains were grown overnight to post-exponential phase (OD 600 3.5-4.5)to a predominantly motile state as described previously 71 .Strain derivatives are described in Supplemental Table 1 and oligonucleotides used in this study are listed in Supplemental Table 2.

Rtn4 Immunoprecipitations
For transfections preceding Rtn4 Immunoprecipitations (IP), HEK293T cells were plated in DMEM medium containing 10% fetal calf serum at a density of 4 x 10 6 cells in a tissue culture-treated 10cm dish.The next day, 12µg of pMT123 DNA (pHA::ubiquitin) was transfected into cells using Lipofectamine 2000, following manufacturer's procedure (Life Sciences).The mammalian HA::ubiquitin expression plasmid pMT123 was a kind gift from D. Bohmann and S. Lippard 59,73 .The next day, transfection medium was removed and replaced with DMEM with 10µM MG132 (Calbiochem) at ~ 30-60min prior to challenge with L. pneumophila at an MOI = 10 for the indicated times.Post infection, cells were lifted and washed in PBS, centrifuged at 4°C at 200 x g, and then cell pellets were immediately frozen on liquid nitrogen and stored at -80°C until used 22 .
For immunoprecipitations, anti-Rtn4 resin was generated by disuccinimidyl suberate crosslinking with anti-Rtn4 (Nogo N-18, Santa Cruz sc-11027) to Protein A/G Plus™ resin at 2µg/µl of packed resin as described in manufacturer protocol (Pierce crosslink IP kit).Samples were lysed in 1% Triton X-100 (20 mM Tris [pH 7], 150 mM NaCl, 5 mM MgCl 2 ) containing protease inhibitor (Roche, Cat# 11697498001) with end-over-end rotation for 20 min at 4°C.Cleared cell lysates were obtained by centrifugation at ≥ 14,000 x g for 10 min at 4°C, diluted with an equal volume of detergent-free buffer, and mixed with washed resin (50% slurry).The mixture was incubated at 4°C via end-over-end rotation for ≥ 4 hr, then washed ≥ 5X in the buffer containing 0.1% Triton X-100 at 4°C.For elution, Rtn4 resin in spin columns was incubated with 0.1M glycine (pH = 2.8) with 0.2% Triton X-100 for 5min, centrifuged, then eluate was reapplied to resin for additional 5 min incubation at room temperature, centrifuged and neutralized with 0.5M Tris (pH = 10.55) 22.Eluate bound to resin was resuspended with reducing SDS sample buffer, boiled, then fractionated by SDS-PAGE coupled with immunoblot analyses.

Protein puri cation
Full-length sdeC genes were ampli ed by PCR and cloned into the bacterial expression vector pQE-80L (Qiagen) with BamHI and SacI restriction sites to generate a 6xHis-SdeC-StrepII fusion construct (Supplemental Table 1).Puri cation was as previously described 22 .Puri ed aliquots were stored at -80°C in 10% glycerol to minimize or eliminate freeze thaw cycle degradation.

Deubiquitinase assay in presence of Sde modi cation
His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scienti c, Cat# 88221) resin at 1 µg enzyme per 25µl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl, pH7.4) for 1 hr at 4°C with end-over-end rotation.The beads were washed 3X with 1X ART buffer to remove nonadsorbed enzyme with microcentrifuge spin lters spun at 3000 x g at room temperature.After the nal wash, each 25µl of pelleted resin was incubated with 2µg of recombinant human K63-linked (R&D systems, Cat# UC-320) or K48-linked (R&D Systems, Cat# UC-220) poly-ubiquitin and 500µM ε-NAD in 300 µl of 1X ART buffer for 2 hrs with end-over-end gentle rotation at 37°C.After incubation, the SdeC or its derivatives-bound resin were removed with microcentrifuge spin column lters, and the polyUb reaction mixture was collected.This polyUb fraction was then incubated with either 1) 100 nM human recombinant PolyHistidine-otubain 1( R&D systems, Cat# E-522B), 2) 50 nM recombinant USP2 catalytic domain (R&D systems, Cat# E-504), 3) 50 nM recombinant PolyHistidine-CYLD (R&D systems, Cat# E-556), or 50nM recombinant SdeC DUB (1-192) at 37°C in 1X ART buffer for 2 hr.A fraction of the reaction was removed, terminated by the addition of reducing sample buffer, and then heated to 50-55°C for 20-30min to avoid Ub chain aggregation.Relative DUB activity was determined by immunoblot probing of SDS-SDS-PAGE fractionated proteins using Image Studio software (LI-COR Biosciences) after 2 hr incubation by comparing the e ciency of cleavage of modi ed Ub relative to unmodi ed.
Bead-binding assay for adaptors association with polyUb post Sde-mediated modi cation of Ub His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scienti c, Cat# 88221) resin at 1µg enzyme per 25 µl of packed resin in 1X ART buffer for 1 hr at 4°C with end-overend rotation.The SdeC adsorbed resin was washed 3X with 1X ART buffer to remove nonadsorbed enzyme with microcentrifuge spin lters centrifuged at 3000 x g at room temperature.After the nal SdeC resin wash, each 25 µl pelleted resin was resuspended with 4µg of recombinant human homotypic-linked polyubiquitin chains Ub 3 − 7 (K63 or K48, 4µg/600µl reaction volume, Boston Biochem, R&D) and 500µM ε-NAD in 1X ART buffer for 2 hrs with gentle end-over-end rotation at 37°C.During incubation of SdeC and polyUb chains, 1µg recombinant PolyHis-p62 was adsorbed to 5 µl pre-equilibrated packed Ni-NTA resin (Thermo Fisher Scienti c, Cat# 88221) via 1 hr, end-over-end rotation at 4°C in low adhesion 1.5 ml microcentrifuge tubes.Polyubiquitin chains were then separated from SdeC by collection of SdeC resin in a microcentrifuge column spin lter and then the polyUb fraction was incubated with recombinant PolyHis-p62 on Ni-NTA resin for 1 hr at room temperature with end-over-end rotation.The resin was washed 3-5X with 50mM Tris 0.1% BSA (vol/vol) pH7.5 at room temperature, then resin having adsorbed proteins and bound polyUb chains were eluted from the Ni-NTA beads with 250 mM imidazole diluted in 50 mM Tris 0.1% BSA (vol/vol) pH7.5.Samples were then analyzed by SDS-PAGE followed by immunoblot.

SPR analysis of Sde-mediated modi cation on binding of poly-Ub to p62
For modi cation of 100 µg of K63-linked Ub tetramer (R&D Systems, Cat# UCB-310) or monomeric Ub were incubated together with 100 µM β-NAD and 20 nM SdeC variants (WT, C118S/DUB − , E859A/ART − and H416A/PDE − ) in 250 µl of 1X ART buffer at 37°C for 1 h.For DUB inhibitor pretreatment, 1 µM ubiquitin-propargylamide (R&D Systems, Cat# U-214) was incubated with 100 nM SdeC variants at room temperature for 30 min before adding to Ub 4 ( nal concentration, 20 nM SdeC in 1X ART buffer).To remove the SdeC, 10 µl of a 50% slurry of Ni-NTA agarose resin (Thermo Fisher Scienti c, Cat# 88221) was washed three times with 1X ART buffer, then added to the Ub-SdeC reaction for another 1hr with endover-end rotation at 37°C.For removal of SdeC enzyme, the samples were centrifuged at 1000 x g for 2 min in a microcentrifuge spin lter column.The supernatant was then desalted using a NAP-5 column (Thermo Fisher Scienti c, Cat# 45-000-151) and lyophilized with a Labconco Freezone12.Samples were resuspended in Ultrapure water to a nal protein concentration of 1 mg/ml.
The binding kinetics between p62 and modi ed Ub 4 was determined by surface plasmon resonance (SPR) using a Biacore T200 system, with a runner buffer of HEPES Buffered Saline (HBS; 10 mM HEPES (pH = 7.4), 150 mM NaCl) containing 30 µM of EDTA.6xHis-tagged p62 was captured onto a Series S NTA sensor chip following manufacturer's recommendations (Cytiva, Cat# 28994951).Brie y, 0.5 mM NiCl 2 solution was injected at a ow rate of 10 µl/min for 60 seconds onto the experimental ow cell, followed by the injection of His-p62 (200 nM) at 5 ul/min for 420 seconds.Ub 4 at various concentrations, that had been previously treated with SdeC derivatives, was injected into the ow cells at a rate of 30 µl/min for 180 seconds, followed by a 300 seconds dissociation phase.Regeneration of the surface was performed by two injections of 350 mM EDTA (30 ul/min, 60 seconds) followed by an injection of 6M guanidine hydrochloride with 50 mM NaCl (30 ul/min, 30 seconds).The binding kinetics were determined with Biacore Evaluation software (v3) using double referenced sensorgrams and 1:1 binding for the tting model.
Immuno of p62 at the LCV BMDMs were plated on glass coverslips at 1-2 x10 5 /well in 24-well plates.Cells were challenged at a multiplicity of infection (MOI) = 2, centrifuged at 200 x g for 5 min, and incubated for the indicated time points.For 180 min infections, the cells were washed 3X in PBS at 60 min post-infection to remove noninternalized bacteria, then fresh culture media was added.At the infection conclusion, all cells were washed 3X in PBS, xed in PBS containing 4% (wt/vol) paraformaldehyde, followed by permeabilization with ice-cold 100% methanol for 20 seconds.Coverslips were then washed 3X in PBS and blocked in PBS containing 2-4% BSA for at least 30 min.Cells were then stained in PBS with rat anti-L.pneumophila and rabbit anti-p62/SQSTM (Abcam, Cat# 109012, 1:400) for 1 hr and detected with donkey anti-rat Alexa Fluor 594 (Jackson ImmunoResearch, Cat# 712-585-153, 1:500) and goat anti-rabbit Alexa Fluor 488 (Jackson ImmunoResearch, Cat# 111-545-003, 1:500).Hoechst 33342 was used to label DNA.

Image analysis
Representative images were taken using Zeiss Axiovert or Nikon inverted phase uorescence microscopes, and image enhanced in Photoshop™ (Adobe), using linear corrections.For quanti cation of total polyUb intensity, Volocity software (PerkinElmer) was used.ROIs (regions of interest, 14 x 12 µm; oval) were set around LCVs, and pixel intensities were measured and subtracted by background intensity.For quanti cation of total p62 intensity, we set ROIs (0.3 x 0.42 inches; oval) around LCVs. Mean p62 intensity/pixel was measured in each ROI and subtracted by background intensity/pixel using Fiji image analysis software 75 .(from reference 52,58 ).(B) Interface residues between UBA domain and Ub, showing possible involvement of Ub R42 in interacting with UBA domain (from reference 52,58 ).(C, D) Ub modi cation at R42 blocks p62 binding (Materials and Methods).Ni-NTA-p62 was incubated with Ub 3-7 that had been treated with SdeC WT or its catalytic mutant derivatives and allowed to bind (In).Unbound Ub 3-7 was removed (FT), the beads were washed, and then eluted with 250mM imidazole (E: elution).The p62-bound poly-Ub in the eluate was fractionated by SDS-PAGE followed by probing with anti-Ub.Relative loading: In, input (2.5%); FT, ow-through (2.5%); E, eluate (25%).

Figure 1 The
Figure 1

Figure 2 The
Figure 2

Figure 3 Sde
Figure 3 (E) Surface plasmon resonance (SPR) quantitation of p62 binding to Ub derivatives.Ub 1 or Ub 4 was incubated with SdeC WT or its derivatives immobilized on Ni-NTA resin, the supernatant containing modi ed Ub derivatives was incubated with 6xHis-tagged p62 bound to a Ni-NTA chip, and binding a nity was measured by plasmon resonance.(F, G) Sensorgrams of Ub 4 or Ub 1 binding to p62.Binding of Ub 4 to p62 yields a K D = 0.97 x 10 -7 M. (H-K) Sensorgrams of Ub 4 , modi ed as noted in panels (red balls: phosphoribosyl addition; green balls: ADP-ribosyl addition).Reactions were formed in the presence of Ub-propargylamide (DUB inhibitor).

Figure 4 ART
Figure 4