A rapid microglial metabolic response controls metabolism and improves memory

Chronic high-fat feeding triggers widespread metabolic dysfunction including obesity, insulin resistance, and diabetes. While these ultimate pathological states are relatively well understood, we have a limited understanding of how high-fat intake first triggers physiological changes. Here, we identify an acute microglial metabolic response that rapidly translates intake of high-fat diet (HFD) to a surprisingly beneficial effect on spatial and learning memory. Acute high-fat intake increases palmitate levels in cerebrospinal fluid and triggers a wave of microglial metabolic activation characterized by mitochondrial membrane activation, fission and metabolic skewing towards aerobic glycolysis. These effects are generalized, detectable in the hypothalamus, hippocampus, and cortex all within 1-3 days of HFD exposure. In vivo microglial ablation and conditional DRP1 deletion experiments show that the microglial metabolic response is necessary for the acute effects of HFD. 13 C-tracing experiments reveal that in addition to processing via β-oxidation, microglia shunt a substantial fraction of palmitate towards anaplerosis and re-release of bioenergetic carbons into the extracellular milieu in the form of lactate, glutamate, succinate, and intriguingly, the neuro-protective metabolite itaconate. Together, these data identify microglial cells as a critical nutrient regulatory node in the brain, metabolizing away harmful fatty acids and liberating the same carbons instead as alternate bioenergetic and protective substrates. The data identify a surprisingly beneficial effect of short-term HFD on learning and memory.


INTRODUCTION
The central nervous system (CNS) plays a critical role in regulating glucose and energy homeostasis by sensing, integrating, and responding to peripheral signals [1].Our understanding of CNS control over energy balance is mostly derived from investigation of distinct functional sets of hypothalamic neurons and their interactions with the periphery [2].In addition, a growing body of evidence supports an important role for glial cells in regulating energy balance [3].For example, astrocytes can regulate energy balance by processing glucose into lactate, which is then released to fuel and modulate surrounding neurons [4], [5], [6], [7].These latter findings demonstrate how metabolic intermediaries from the neuronal niche can directly influence CNS output.
Microglia are the third most abundant glial cell in the brain.Developmentally, they are derived from the yolk sac and are considered CNS immune cells.Like the peripheral immune compartment, microglia activation has been implicated in metabolic disease pathogenesis [8], [9], [10].Specifically, microglia in the mediobasal hypothalamus (MBH) become activated under conditions of chronic high-fat feeding in a process termed 'microgliosis'.High fat diet (HFD)-induced activation has been associated with moderate increases in cytokine gene expression [8,11] and pioneering studies testing both genetic manipulation of microglial NF-kB and microglial depletion with PLX5622 suggest that microglial activation is necessary for the metabolic impairments triggered by chronic HFD [9].Recent evidence suggests a role for microglial mitochondrial alterations (fission) that can be detected within days of high fat diet (HFD) administration [10].Microglia outside the hypothalamus also activate upon chronic HFD exposure [12,13] and associate with cognitive impairment [14,15].A key outstanding question in the field, then, is how high-fat feeding first triggers microglial activation.
Here, we find that microglia rapidly and directly sense the switch to dietary fat within a single feeding cycle.By three days, HFD increases microglial mitochondrial membrane potential, inhibits complex II activity, and skews mitochondria towards fission.Using cerebrospinal fluid (CSF) metabolomics we demonstrate that microglia are directly exposed to increased palmitate and stearate levels within hours of the feeding switch, and using 13 C-tracer studies, we find that microglia robustly metabolize fatty acids via β-oxidation.Interestingly, we find the same acute microglial metabolic response (aMMR) beyond the hypothalamus in vivo, in the hippocampus and cortex.aMMR is largely independent of transcriptional changes, and intriguingly, we find that it improves spatial and learning memory.Microglial depletion experiments and microglia-specific DRP deletion show that microglia and more specifically microglial mitochondrial fission, are necessary for the immediate glucose homeostatic responses and memory improvements to HFD.The data thus identify an acute non-transcriptional microglial metabolic pathway that couples detection of acute increases in dietary fat to changes in whole body metabolism and memory.

Acute HFD-induced metabolic changes are microglia dependent
To understand the nature of the immediate high-fat feeding response, we characterized the physiological effects of acute HFD (3d ad libitum feeding) in cohorts of C57BL/6J mice (Fig 1A).Metabolically healthy mice exhibit rapid metabolic shifts on this timescale, including fasting and post-absorptive (2-hr fast) hyperglycemia and an elevated insulin response to glucose (Fig 1B-E, Suppl Fig S1A-B) [16,17].Glucose excursions, as revealed by baseline-correction, and insulin tolerance test results are minimally impacted upon acute HFD (Suppl Fig S1C ), an important contrast to chronic HFD [18].These acute changes in glycemic control are transient, returning to homeostatic levels within one week of a return to normal chow ('Reverse Diet'; Fig 1C -E) and are not associated with substantial body weight gain (Suppl Fig S1D).To test whether microglia contribute to the induction of these acute HFD-induced effects, we treated mice with the microglia-ablating drug PLX-5622 [19] for 7-9 days and repeated the 3d HFD metabolic assessment.PLX-5622 treatment depleted hypothalamic microglia by >95% across animals (Fig 1F) and prevented both the acute HFD-driven insulin hypersecretion and post-absorptive glucose excess (Fig 1C-E).Hyperglycemia associated with over-night fasting was unaffected by microglial depletion (Fig 1C).The latter indicates that acute HFD triggers microglia-dependent and independent metabolic effects.Thus, microglia are required for metabolic changes induced by the transition to high-fat feeding.

A rapid Microglial Mitochondria Response to high fat diet
Long-term exposure to HFD has previously been associated with hypothalamic microgliosis (increased number and activation of CD45 lo ;CD11b + microglia) and monocyte infiltration (CD45 hi ;Cd11b + ) [9].Examination of 3d HFD responses in both wild-type animals and animals harboring a microglia-restricted EYFP lineage reporter showed no evidence of either increased hypothalamic microglial proliferation (Suppl Fig S1E ) or monocyte infiltration (Suppl Fig S1F).Similarly, using one of the most sensitive scRNAseq protocols available (CEL-Seq2), we found no evidence of a significant microglial transcriptional response upon 3d HFD.The latter analysis included searches for changes in heterogeneity, skewing across sub-states, and appearance of new cell sub-states (Fig 1G,Suppl Fig S1G).The lack of response is especially clear when juxtaposed to experimental autoimmune encephalomyelitis (EAE)triggered responses performed using the same purification and sequencing protocols in the same laboratory (EAE; Fig 1G ).Thus, the central effects of acute HFD are distinct from those of chronic HFD, and have little to no effect on microglial expansion, transcription, heterogeneity, or inflammation in the hypothalamus.
A deep body of literature has highlighted how cellular metabolic changes are necessary and sufficient mediators of cell-type specific function, work pioneered recently by the immunometabolism community [20].We tested whether such changes in cellular metabolism might underpin the microglial response to acute high-fat feeding.Interestingly, mitochondrial membrane potential was increased in primary MBH microglia sorted from animals administered a 3d HFD (Mitotracker Deep Red; Fig 2A -B).Co-staining in the same samples showed no evidence of altered mitochondrial mass (Mitotracker Green; Fig 2C).This acute microglial metabolic response (aMMR) was detectable within 12 hours of high-fat feeding (Fig 2B).aMMR appeared transient and distinct from the effects induced by chronic HFD.Specifically, parallel measures showed no induction of membrane potential in 4-week HFD animals (Fig 2B).Thus, microglia respond to acute dietary fat through a relatively restricted mitochondrial response.
To understand the inputs that might trigger the aMMR response, we performed targeted metabolomics of cerebrospinal fluid (CSF) (Fig 2D; Suppl Table 1).Overall, relatively few metabolites change upon 3d HFD.Nicotinamide-N-oxide was the only metabolite that decreased significantly.Six metabolites increased upon 3d HFD including methylmalonate, isovalerylcarnitine, nicotinic acid, glutarylcarnitine, and notably, the fatty acids palmitate (hexadecanoic acid) and stearate (octadecanoic acid).Palmitate and stearate, the only two metabolites that changed by >2-fold, are highly enriched in the HFD itself (Research Diets D12492) suggesting that microglia respond directly to dietary fatty acid levels.
To test whether these observed fatty acid changes in the CSF might directly trigger aMMR, we examined the primary microglial response to palmitate in vitro using neonatal microglia (Fig S2A-B).Palmitate had no effect on either baseline or maximal oxygen consumption under standard culture conditions (Fig 2E).That said, when added in conjunction with succinate (to prime complex II-mediated OxPhos), palmitatetreated microglia were unable to utilize succinate.Acute palmitate therefore appears to rapidly compromise or saturate complex II function and prevent utilization of substrates routed through complex II.Consistent with these in vitro findings, fresh primary microglia from 3d HFD animals failed to respond to complex II-specific mitochondrial stimuli (Rotenone + Succinate;

aMMR is required for diet induced homeostatic rewiring in vivo
We next tested whether microglial mitochondrial dynamics are required for the in vivo metabolic responses to 3d HFD.We generated tamoxifen-inducible, microglial-Drp1 knockout mice (Drp1 MGKO ) by crossing Drp1 fl/fl animals with a Cx3cr1 creER transgenic line [21,22].Drp1 MGKO animals were born at Mendelian ratios and grow normally.Tamoxifen injection followed by 4 weeks washout generated the intended Drp1-deletion (Fig 3 A-B, Suppl Fig 3A) [22].
Body weight, fat mass and lean mass regulation were normal both before and after tamoxifen injection in Drp1 MGKO mice, indicating that microglial DRP1 (and by extension microglial mitochondrial fusion) is dispensable for body weight regulation under standard conditions (Suppl Fig 3B -C).These data are consistent with literature suggesting that microglia do not substantially participate in body mass regulation [23].Drp1 MGKO mice also showed normal glucose and insulin tolerance (Fig 3D ) indicating that microglial mitochondrial dynamics are also dispensable for baseline control of glucose homeostasis.Importantly, whereas 3d HFD triggered post-absorptive hyperglycemia and enhanced glucose-induced insulin release in control animals, Drp1 MGKO failed to mount a comparable whole body metabolic response (Fig 3E).Consistent with the data from Fig 1, the 3d HFD-induced-hyperglycemia that is observed under overtly catabolic conditions, which we found to be microglia independent (Fig 1C), was still present in Drp1 MGKO animals (Fig 3E).These data reinforce the conclusion that the metabolic responses to acute HFD are highly specific and include both microglia-dependent and independent mechanisms.Thus, microglial mitochondrial dynamics are required for the immediate in vivo homeostatic response to 3d HFD.

Microglia take up and metabolize free fatty acids
Based on BODIPY fluorescence, we found that primary microglia increase lipid droplet numbers within 24h of in vitro exposure to palmitate (200uM; Fig 4A), suggesting a capacity to take up and process fatty acids.To characterize the microglial consequences of fatty acid uptake we used stable isotopic tracer labelling and directly quantified U-[ 13 C]-palmitate ( 13 C-palmitate) processing in both control (BSA treated) and 24h palmitate pre-exposed microglia.Primary microglia incubated with 13  .This is a relatively unique pattern of substrate routing.Thus, microglia store and metabolize palmitate for both energy-producing and anaplerotic reactions.

Palmitate induces a novel microglial lactate/succinate/itaconate release pathway.
We next evaluated the effect of the cold palmitate pretreatment on substrate routing and utilization, our model for understanding aMMR.Palmitate pretreatment interestingly increased labelling of palmitoylcarnitine, acetylcarnitine and acetylserine, demonstrating that 24h palmitate exposure accelerates β-oxidation ( indicating that fatty acid exposure further increases microglial substrate routing to glutamate and anaplerosis.Tracer measurements made in extracellular media conditioned from the same cells revealed that 24h palmitate treatment triggered release of glutamate, succinate, and intriguingly itaconate (Fig 4G-I), an immunomodulatory metabolite known to inhibit succinate dehydrogenase (Complex II) [24] and recently shown to be neuroprotective [25].These findings are consistent with the complex II inhibition observed above upon 3d HFD/palmitate treatment and suggest that itaconate-dependent inhibition of complex II inhibition limits the bioenergetic utilization of fatty acids by microglia, sparing the carbons for release to the cell exterior.Collectively, the data therefore indicate that microglia sense and process fatty acids to generate alternative metabolites for surrounding cells (Fig 4O).
We next tested whether the effects of 24h palmitate exposure were specific to palmitate-derived carbons or generalizable to other substrates.We repeated the same experiment, instead replacing U-[ Collectively, these data identify microglia as a novel metabolic node able to take-up potentially harmful free fatty acids and repurpose them to fuel surrounding cells in the form of lactate and anaplerotic substrates (Fig 4O).

Acute HFD induces widespread MMR and rapid modulation of spatial and learning memory
Changes in neuronal function have previously been linked to lactate provided by glucose metabolism in astrocytes [26].To determine if the substrates released by microglia have the potential to directly influence neurons, we incubated primary neuron cultures (Suppl Fig S5A ) with conditioned media from the microglial 13 C-glucose tracing experiments (ie.media containing 13 C-glucose-derived isotopically labeled lactate, itaconate, succinate, citrate).As a control for direct uptake of residual 13 C-glucose, we treated parallel neuronal cultures with the fresh 13 C-glucose tracing media originally added to the microglia.Intriguingly, and consistent with literature documenting poor direct glucose utilization by neurons [27], we found robust m+3 lactate (and other metabolites) in cells treated with microglial conditioned media, at levels that far exceeded labelling triggered by glucose tracer alone (Fig 5A, middle column vs left column)(Suppl Fig S5B).These data demonstrate that microglia can contribute carbon substrate for neuronal use, and, they highlight the relative importance of this process compared to uptake of pure glucose.The data identify a route through which aMMR likely alters neuronal function.
The majority of literature relating HFD-associated microglial function to metabolic regulation is focused on the hypothalamus, a region of the brain that exhibits an unusually leaky blood brain barrier [8,28].Given our findings that CSF free fatty acid levels double upon acute HFD and given the dramatic metabolomic rewiring induced by microglial palmitate exposure, we asked whether aMMR might also occur in other brain regions.We FACS-sorted cortical and hippocampal microglia and tested for mitochondrial activation (MMR) in 3d HFD exposed mice.Indeed, 3d HFD exposure triggered an increase in mitochondrial membrane potential in both cortical and hippocampal microglia (Fig 5B-C).These data were consistent with the CSF results and suggested therefore that acute high-fat feeding might alter higher cognitive function.
Given the hippocampal signature, we tested whether 3d HFD mice showed any deleterious memory phenotype compared to chow-fed controls.Surprisingly, HFD-treated animals outperformed the controls.Using a standard Barnes test, 3d HFD mice exhibited a faster reaction (Fig 5D), suggestive of improved learning and spatial memory.Similarly, when challenged with a T-Maze, 3d HFD exposed animals exhibited heightened spatial memory (Fig 5E).These phenotypes were reproducible at two different institutes (VAI, USA and MPI-IE, Germany) and in the hands of different experimentalists (Fig 5D-E, Suppl Fig S5C-D).Notably, the memory differences were observed in the absence of any detectable changes to motor coordination (ROTAROD; Fig 5F -H).Thus, acute high-fat feeding triggers improvements in learning and spatial memory.
To validate that these 3d HFD induced cognitive effects were microglial aMMR dependent, we once again repeated experiments in microglia-depleted (PLX-5622) and Drp1 MGKO mice.Importantly, PLX-5622treated animals failed to show any detectable memory improvements upon a 3d HFD (Fig 5I-J) indicating indeed that an intact microglial compartment is necessary for acute HFD enhancement of memory function.Likewise, 3d HFD exposure failed to trigger memory improvements in Drp1 MGKO mice (Fig 5K ) indicating that the memory enhancing response, like the glucose homeostatic effect, is microglial DRP1dependent.Again, no mice in any treatment group showed altered motor effects by ROTAROD (Suppl FigS5 E-J).Therefore, 3d HFD-triggered aMMR appears to constitute a generalized physiological response that optimizes dietary coupling to diverse central functions.

DISCUSSION
Our data identify a new role for microglial cells as a rapid metabolic sensor of dietary macronutrient composition, and as a relay that triggers cognitive and peripheral metabolic responses.One of the most intriguing results was the directionality of the rapid metabolic responses.Whereas a bulk of literature demonstrates adverse outcomes upon chronic HFD, both in the brain (microgliosis, inflammatory cytokine release, myeloid cell infiltration) and periphery (hyperglycemia, insulin resistance) [8,9], our findings indicate that acute HFD initiates a distinct homeostatic response that supports cognitive function.From an evolutionary perspective, there are obvious benefits to partitioning energy use towards cognition once energy demands are well met.We find that aMMR results from direct uptake, processing, and release of fatty acid derived carbons, and demonstrate that microglia are capable of metabolizing fatty acids towards diverse intracellular and extracellular pools.One immediate question raised by this work is the mechanistic nature of the flip from the seemingly beneficial acute MMR (aMMR) observed here, to the well-documented inflammatory MMR (iMMR) triggered after 1-2 weeks of chronic HFD.One possibility is that the response requires flexibility between lipid storage and processing, and that this becomes saturated with prolonged HFD.
Of equal interest are the PLX5622 experiments and data, which demonstrate for the first time that microglial ablation has no overt effect on baseline metabolism and memory.Importantly, however, the data show that microglia are critical for optimal response to dietary change.We are aware of the fact that CSFR1 inhibiting compounds such as PLX5622 deplete not only microglia but also long living CNS macrophages in the meninges and perivascular space [29] but they are by far less numerous and placed far from neurons in the parenchyma.We demonstrate a novel role for microglia in optimizing glycemia upon short-(2 hours) but not long-(24 hour) term fasting and therefore suggest that microglia mediate a glucose-sparing function in the transition from the post-prandial to fasted state.Future work mapping the downstream neuronal circuitry governing this response will be important.The overabundance of food in since industrialization has skewed a significant global population towards chronic prandial and postprandial states and away from one of substantial intermittent fasting events.The finding that aMMR coordinates central metabolic balance with peripheral glucose homeostasis selectively during the prandial/post-prandial phase adds nuance to a significant literature distinguishing these physiological contexts [30], [31], [32]).From an evolutionary perspective it is easy to rationalize why initial periods of plenty would be mechanistically coupled to maximizing central functions.
One question raised by these findings is if there is a hierarchy for substrate sharing between glial cell (microglia, tanycytes, oligodendrocytes and astrocytes) and neurons.Our data indicate that microglia provide several TCA-associated substrates into the extracellular milieu, and, that palmitate/HFD increases this activity.Tanycytes and astrocytes have both been documented to release select metabolites into the extracellular environment [33,34].Given that CSF production is only loosely compartmentalized (e.g.relative to the highly physically structured control of blood), we suspect a cooperative model over hierarchical, serial or synergistic metabolic compartmentalization models.Other new questions relate to the depth or breadth of other cognitive processes that might also be influenced by acute HFD.In this study, we demonstrate that aMMR involves release of at least 4 important anapleurotic substrates, and that aMMR is detectable from the hypothalamus to the hippocampus and cortex.Increased lactate has been shown to enhance fear memory in a glutamate-associated mechanism ( [35]).Both lactate and glutamate are increased upon 3d HFD-induced aMMR.
In addition to identifying novel substrate routing patterns in microglia, our data demonstrate that mitochondrial fission is necessary for aMMR.Fatty acid triggered fission has been observed in several cell types [36] including neurons and glial cells [37], [38], [10].Work by Kim et al. [10] suggests that the aMMR characterized here may require UCP2; those authors demonstrated UCP2-dependent mitochondrial changes in short-and long-term HFD exposed hypothalamic microglia.Collectively the two studies argue against the notion that astrocytes are unique amongst glial cells in their ability to use fatty acids, and importantly, to release fatty-acid derived substrates for use by neurons and other neighboring cells [39].
Finally, this study demonstrates previously unappreciated plasticity and complexity for microglial cellular metabolism.Rather than being largely glucose-constrained, our tracer studies reveal that primary microglia from across the brain readily take-up, metabolize and store fatty acids and that fatty acids are metabolized towards bioenergetic and acetylation reactions (acetyl-coA).They prove for the first time that substantial b-oxidation occurs in microglia cells even in the presence of high extracellular glucose [40].The coordinate observations of intra-and extracellular succinate and itaconate accumulation and relative Complex II inhibition indicate that palmitate metabolism drives feed-forward inhibition of Complex II via itaconate production and shunting of TCA intermediates out of the cycle towards glutamate.Further examination of the role of glutamine as a fuel and anapleurotic regulator of cellular function is therefore warranted.At least under the standard conditions used here and, in the field, microglia appear to be metabolically wired release of carbon substrates to neighboring cells, through several intermediates including lactate, succinate, glutamate, citrate and itaconate.This idea is consistent with the lack of substantial induction of lipid droplet accumulation, respiration, or ROS buildup upon palmitate exposure.
In conclusion, we identify a rapid microglial mechanism that translates direct dietary fat sensation into a non-transcriptional, mitochondrial response that specifically alters extracellular regulatory substrates (lactate, glutamate, itaconate and succinate) and enhances memory function.The response is progressive over the first days of dietary intervention, an effect that appears dependent on feed forward inhibition of mitochondrial complex II by itaconate.The aMMR response is detectable across the entire brain, which carries important implications for understanding how our diet directly regulates diverse cognitive and homeostatic responses, and the development of pharmacological and lifestyle interventions to enhance them.CRC/TRR167 and the Marie Skłodowska-Curie Postdoctoral Fellowship (EPOC -707123).The team is supported by National Institutes of Health awards R21HG011964 and 1R01HG012444.

Experimental Animals
All animal studies and experimental procedures were approved by the either the Max Planck Commities in Germany or by the Van Andel Institute Institutional Animal Care and Use Committee in US, before project initiation.All mice were housed in a temperature-controlled environment (23°C) with a 12-h light and 12-h dark (07.00-19.00)photoperiod.Animals were provided standard chow diet (Research Diets #5021; 23,6% calories from fat; Mouse Breeder Diet #6539) or HFD (60% fat; Research diet, Rodent Chow #D12492) and water ad libitum unless otherwise stated.

Microglial Depletion
Microglia were depleted in all cases by feeding mice custom diet (Research Diets) containing the CSF1R inhibitor PLX5622 (Plexxikon) formulated in the exact diets indicated above.The compound-containing diets have the new order numbers (D12450: PLX5622-containing standard diet and D21021905: PLX5622-containing high-fat diet).PLX5622 was included to achieve a dose of 1.2 g/kg based on average adult C57BL/6J food intake.Animals were administered the diet for 7-9 days.

Microglia Cultures
Eight to ten mice (1-3 days old, mixed sex) from C57BL/6J mice were used for primary microglia culture.In brief, brains were dissected and placed in a small culture dish that contained a small volume of Dulbecco's modified eagle medium (DMEM).The meningeal lining (dura and arachnoid layers) were then gently removed using two small straight forceps under a magnifying glass.The cleaned brain was then placed in a new culture dish minced into a fine slurry using a pair of corneal scissors.Suspended cells were filtered (70 μm) and centrifuged for 5 min at 1000 rpm and the pellet was re-suspended and plated on poly-D-lysine (Cat# P6407, Sigma)-coated 75cm 2 flasks with DMEM containing 1% penicillinstreptomycin, 10% fetal bovine serum.Seven to 10 days later, the flasks were stimulated for 3 days with 30ng/ml M-CSF (Cat# 315-02, Peprotech) and then, shaken (200 rpm) for 3 hours (37°C) to specifically release microglia.

Embryonic Cortical Neuron Culture Protocol
Primary neuron cultures were prepared from embryonic day 18 (E18) CD1 mice.Brains were gently removed from the embryos and placed into a petri dish filled with ice cold, sterile Hibernate Medium (Cat#.A1247601, Gibco).Hemispheres were gently separated, and the meninges, thalamus, striatum, brainstem, and hippocampus were removed.Cortical tissue was isolated and cut into 1 mm 3 segments.The cortical tissue from each brain was pooled and digested in papain solution (20 U/mL Cat# LS003126, Worthington) and then treated with DNase I (Cat# LS006563, Worthington) to remove residual DNA.The tissue was then washed with pre-warmed Neurobasal media (Cat# 21103049, Gibco), mechanically dissociated, and strained through a 40 μm cell strainer.The cell suspension was pelleted at 1000 rpm for 5 minutes, resuspended in 2 mL of neuron media (Neurobasal media containing 1% B27, 2mM GlutaMAX and penicillin-streptomycin) and gently mixed.The dissociated neurons were seeded on poly-D-lysine coated 6 well culture plates at 1 million cells/ well.On DIV3, cytosine arabinoside (1-β-Darabinofuranosylcytosine) was added to a final concentration of 5 μM to curb glial proliferation.The neurons were maintained until DIV21 by replacing 1/3 volume of media with fresh neuron media every five days.

Microglia Isolation
Mice were anesthetized and blood was collected by ventricular puncture.Mice were then perfused with phosphate-buffered saline (PBS).For microglia isolation, the hypothalamus, cerebellum, hippocampus, and cortex were dissected from the brain and manually dissociated in HBSS buffer.A cell suspension was prepared with a continuous 37% Percoll gradient at 2300 rpm for 30 min without brake, then cells were washed with 1x PBS and resuspended in FACS buffer and non-specific binding to Fc receptors was blocked by incubation with anti-CD16/32 antibody (BD Pharmingen, Cat# 553141) and the FACS antibodies BV711 conjugated anti-CD11b and Pacific blue conjugated anti-CD45 antibodies (Cat# 101241, Cat# 103126, BioLegend).

scRNA-seq analysis
The scRNA-seq libraries were analyzed using the 'scRNAseq' module from snakePipes v.1 [45].Within the snakePipes pipeline, trimming of barcodes was achieved using Cutadapt v.1.9.1 [46].Quality of the reads was evaluated with FastQC (https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).Mapping to the GRCm38/mm10 mouse genome was performed using the STAR aligner v.2.5.3a [47], and read counts on features were quantified by featureCounts v.1.6.1 [48].Mapped and counted read were next analyzed with RaceID3 v.0.2.6, with default parameters [44,49].Batch correction by plate, and preprocessing of data were completed within the RaceID3 pipeline.We included in our analysis, all microglia cells from control (CT) and 3-day high fat diet treated (3d_HFD) mice.We filtered out all the cells with a total transcript count per cell >1000.Dimensional reduction of transcriptomic profiles was then preformed with Uniform Manifold Approximation and Projection (UMAP).As a comparative analysis, we also added to our dataset, previously published single microglial cells from experimental autoimmune encephalomyelitis (EAE), and matched controls mice (CT_EAE) [42].

Data availability
The scRNA-seq raw data, processed count matrices and related metadata have been deposited to Gene Expression Omnibus (GEO) and are publicly available under the accession codes GSE217045.qPCR For the qPCR experiments, pure microglia cells were sorted, and the RNA was extracted with Trizol (ImGen protocol).

Mitochondrial Function by Cytometry
For the mitochondrial activity experiments, the cell suspension was incubated with the mitochondrial probes MitoTracker Green FM (Invitrogen, Cat# M7514) and MitoTracker DeepRed FM (Invitrogen, Cat# 22426) for 30min at 37degrees before FACS acquisition.For the electron transport chain experiments (ETC), the cell suspension was incubated with the mitochondrial probe Tetramethylrhodamine TMRM (Abcam, Cat# ab228569) and fluorescent glucose analog 2-NBDG (Abcam, Cat# 235976) for 30min at 37degrees before FACS acquisition.For challenging the ETC, the cell suspension was incubated each 90 seconds by the following drugs: 0,5ul of 100uM Rotenone (Sigma), 2ul of 2.5M Succinate (Sigma) and 0.5ul of 1mM Antimycin (Sigma).Cytometry was performed on Fortessa (BD Bioscience) and analyzed with FlowJo v 10 (Treestar).Microglial cells were gated from CD45low-CD11b+ cells followed by singlet after forward and side scatter pattern.
After several washes with PBS, sections were incubated in the appropriated secondary antibodies (thermofisher) for 1 hr at room temperature, then rinsed in PBS three times 10 min each time, and flatembedded in mounting media with DAPI (Fluoroshield, Abcam; Cat#104139).The Pictures acquisition were performed with the Confocal LSM780 (Zeiss).

Glucose and Insulin Tolerance
OGTTs were performed in 16 hours fasted animals.After determination of basal blood glucose levels, each animal received a gavage of 1g/kg glucose (Sigma) and blood glucose levels were measured at -30, 0, 15, 30, 45, 60, 90 and 120 min after glucose administration using a glucometer (Accu-Check, Roche).
In the same cohort of mice, blood samples were also collected for determination of circulating insulin levels during the OGTT at -30, 15, 30 and 60 with the Elisa insulin assay kit (Mercodia).ITTs were performed in 2 hours fasted animal.After determination of basal blood glucose levels, each animal received an i.p. injection of Insulin, 0.75 U/kg (Actrapid, Novo Nordisk).Blood glucose levels were measured at -30, 0, 15, 30, 45, 60, 90 and 120 min after insulin administration.

Body Composition
Body composition was measured in vivo by MRI (EchoMRI; Echo Medical Systems, Houston, TX).

Behavioral Tests
Mice were fasted at 7:00 AM and the behavioral tests were performed between 8:00 AM and 2:00 PM.ROTAROD, Barnes and T Maze behavioral tests were performed as previously described [50] [51].

Microglial Analysis
To analyze microglial number and size in the ARC, VMH and cortex, tdtomato positive cells were counted manually from 4 hypothalamic level-matched sections per animal using ImageJ software and microglia cell size was measured using a thresholding parameter on ImageJ software.A total of 40 cells per section were used to determine the size in all regions.

Measures of Mitochondrial length
Primary microglial cells were seeded overnight in Poly-D-lysine-coated cell culture chambered coverslips (Lab-Tek Cat#155411, Thermo scientific) at 1x10 4 cells/well density in 100 μl microglial growth media.Then, they were incubated at 37 for 2, 6 or 24 hrs in microglial growth media completed with 100uM BSA or 100uM Palmitate complexed with BSA [52] or 100uM Oleate complexed with BSA or 1ug/ml LPS [53].Half hour before the end of the incubation, 200nM MitoTrakerGreen (Invitrogen, Cat# M7514) was added in the media.The cells were washed 3 times with their own incubation media and the mitochondrial network was observed in vivo cells with Confocal LSM 780 (Zeiss).
After pictures acquisition, 5uM MitoSox (Invitrogen, Cat# M36008) were added in the incubated media for 10min.Cells were washed 3 times with media and fixed with 4%PFA at 37 for 15min.The ROS staining was observed with Confocal LSM 780 (Zeiss).

Cytokine measurement
Media from primary Microglia incubated with BSA or Palmitate was collected and processed using the mouse Custom Panel Standard kit (#93976 LegendPlex)

Stable isotope labeling and metabolomics
Metabolomics was performed on microglia by first washing microglia primary cell cultures with PBS and re-cultured in DMEM (lacking glucose) containing 10% dialyzed FBS and uniformly labeled [ 13 C]-Glucose (Cambridge Isotope Laboratories).Microglia (2 x 10 6 per well in 6 well plates) were cultured in 13 C-containing medium for up to 6 hours.For cellular media samples, 10 µL of media were taken at indicated time points and centrifuged to remove cells, with 10 µL of media used for metabolite analysis.
For uniformly labeled [ 13 C]-Palmitate tracing analysis, sodium [ 13 C]-Palmitate was conjugated to BSA following Agilent Seahorse Palmitate-BSA conjugation protocol.For [ 13 C]-Palmitate tracing, microglia primary cell cultures were first cultured for 24hr with BSA control or 12 C-Palmitate (200uM) in DMEM containing 10% dialyzed FBS.Following 24hr incubation, the media was removed, and microglia cells washed with PBS followed by culture in DMEM with 10% dialyzed FBS and blank mM [ 13 C]-Palmitate for 4 hours and cell and media samples collected as before.
For Gas chromatography coupled to mass spectrometry (GC-MS) metabolites were extracted using ice cold 80% methanol, sonicated, and then D-myristic acid was added (750ng/sample) as an internal standard.Dried samples were dissolved in 30 μL methozyamine hydrochloride (10mg/ml) in pyridine and derivatized as tert-butyldimethylsily (TBDMS) esters using 70 μL N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) [54].For metabolite analysis, an Agilent 5975C GC/MS equipped with a DB-5MS+DG (30 m x 250 µm x 0.25 µm) capillary column (Agilent J&W, Santa Clara, CA, USA) was used.All data were collected by electron impact set at 70 eV.A total of 1 µL of the derivatized sample was injected in the GC in splitless mode with inlet temperature set to 280˚C, using helium as a carrier gas with a flow rate of 1.5512 mL/min (rate at which myristic acid elutes at 17.94 min).The quadrupole was set at 150˚C and the GC/MS interface at 285˚C.The oven program for all metabolite analyses started at 60˚C held for 1 min, then increased at a rate of 10˚C/min until 320˚C.Bake-out was at 320˚C for 10 min.Sample data were acquired both in scan (1-600 m/z) modes.MassHunter software (v10, Agilent Technologies) was used for peak picking and integration of GCMS data.Peak areas of all isotoplogues for a molecular ion of each metabolite were used for mass isotopomer distribution anaylsis using a custom algorithm developed at VAI.Briefly, the atomic composition of the TBDMS-derivatized metabolite fragments (M-57) was determined, and matrices correcting for natural contribution of isotopomer enrichment were generated for each metabolite.After correction for natural abundance, a comparison was made between non-labeled metabolite abundances ( 12 C) and metabolite abundances which were synthesized from the 13 C tracer.Metabolite abundance was expressed relative to the internal standard (Dmyristic acid) and normalized to cell number.
For liquid chromatography coupled to mass spectrometry (LC-MS) metabolites Metabolites were analyzed for relative abundance by high resolution accurate mass detection (HRAM) on two QExactive™ Orbitrap mass spectrometers (Thermo Fisher Scientific) coupled to Thermo Vanquish liquid chromatography systems.Separate instruments were used for negative and positive mode analysis.For negative mode analysis, an Acquity T3 HSS (1.8 µm, 2.1 mm x 150 mm) column (Waters, Eschborn, Germany) was used for chromatographic separation and the elution gradient was carried out with a binary solvent system.Solvent A consisted of 3% methanol, 10 mM tributylamine, and 15mM acetic acid in water (pH 5.0 +/-0.05) and solvent B was 100% methanol.A constant flow rate of 200 μL min −1 was maintained and the linear gradient employed was as follows: 0-2.5 min 100% A, 2.5-5 min increase from 0 to 20% B, 5-7.5 min maintain 80% A and 20% B, 7.5-13 min increase from 20 to 55% B, 13-15.5 min increase from 55 to 95% B, 15.5-18.5 min maintain 5% A and 95% B, 18.5-19 min decrease from 95 -0% B, followed by 6 min of re-equilibration at 100% A. The heater temperature was set to 400° C and ion spray voltage was set to 2.75 kV.The column temperature was maintained at 25 °C and sample volumes of 10 µL were injected.A 22-minute full-scan method was used to acquire data with m/z scan range from 80 to 1200 and resolution of 70,000.The automatic gain control (AGC) target was set at 1e6 and the maximum injection time was 500 ms.For positive mode analysis, an Atlantis T3 (3 µm, 2.1mm ID × 150mm) column (Waters) was used and the he elution gradient was carried out with a binary solvent system Solvent A consisted of 0.1% acetic acid and 0.025% heptafluorobutyric acid in water and solvent B was 100% acetonitrile.A constant flow rate of 400 μL min−1 was maintained and the linear gradient employed was as follows: 0-4 min increase from 0 to 30% B, 4-6 min from 30 to 35% B, 6-6.1 min from 35 to 100% B and hold at 100% B for 5min, followed by 5 min of re-equilibration.The heater temperature was set to 300° C and the ion spray voltage was set to 3.5 kV.The column temperature was maintained at 25 °C and sample volumes of 10 µL were injected.An 11-minute full-scan method was used to acquire data with m/z scan range from 70 to 700 and resolution of 70,000.The automatic gain control (AGC) target was set at 1e6 and the maximum injection time was 250 ms.Instrument control and acquisition was carried out by Xcalibur 2.2 software (Thermo Fisher Scientific).Full scan LC-MS data were analyzed in Compound Discoverer (v 3.2, Thermo Scientific).Compounds were identified by chromatography specific retention time of external standards and MS 2 spectral matching using the mzCloud database (Thermo Scientific).

Quantification and Statistical Analysis
Two-way ANOVA was used to determine the effect of the genotype and treatment with the Prism 7.01 software (GraphPad Software).For repeated measures analysis, ANOVA was used when values over different times were analyzed.When only two groups were analyzed, statistical significance was determined by an unpaired Student's t-test.A value of p < 0.05 was considered statistically significant.All data are shown as mean ± SEM unless stated otherwise.We did not include additional statistical tests for data distributions.G. sgRNAseq dataset from hypothalamic microglia cells harvested from C57bl6/J male mice fed with control diet (CT) and High Fat Diet for 3 days (HFD_3d) (n=5) merged with sgRNAseq microglia dataset from mice presenting an Experimental Autoimmune Encephalomyelitis (EAE).Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc test.C. Graph showing the Mitotraker Green fluorescence from sorted hypothalamic microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for for 12 hours,3 days or 1-4 weeks (n=5 to 12).D. Volcano plot showing the metabolites content of cerebrospinal fluid from of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (n=10).E. Seahorse (+/-succinate added in the media during the experiment) on primary microglia challenged for 24hours with BSA (control) or Palmitate (experiment replicated 3times).F. Mitochondrial Electron transport chain activity recorded with FACS after TMRM staining from sorted microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (n=5).G. Mitochondrial networks from primary microglia stained with Mitotraker green after being challenged for 24hours with BSA (control), Palmitate, Oleate or LPS (n=40) and the mitochondrial length quantification graphs.
H. DRP1 colocalization with the mitochondrial network stained with TOMM20 on primary microglial cell after being challenged for 24hours with BSA (control) and Palmitate(n=40) and the colocalization quantification graphs.
M. 13 C-glucose incorporation into succinate (m+2) released during the 6 hours tracing experiment by primary microglia pretreated for 24hours with BSA or palmitate (n=3).N. 13    A 13 C-palmitate incorporation into palmytoilcarnitine and acetylcarnitine after 4 hours tracing experiment on primary microglia pretreated for 24hours with BSA or palmitate (n=3).The results are graphed in relative abundance.B. 13 C-palmitate incorporation into acetyl-serine after 4 hours tracing experiment on primary microglia pretreated for 24hours with BSA or palmitate (n=3).The results are graphed in relative abundance.C. 13 C-palmitate incorporation into aconitate, alpha-ketoglutarate, fumarate, malate after 4 hours tracing experiment on primary microglia pretreated for 24hours with BSA or palmitate (n=3).The results are graphed in relative abundance.D. 13 C-palmitate incorporation into glutamate after 4 hours tracing experiment on primary microglia pretreated for 24hours with BSA or palmitate (n=3).The results are graphed in relative abundance.E. 13 C-glucose incorporation into pyruvate, citrate, alpha-ketoglutarate, succinate, and malate after 6 hours tracing experiment on primary microglia pretreated for 24hours with BSA or palmitate (n=3).
Fig 2F) validating the findings in adult, ex vivo contexts.Consistent with the in vivo observations above, in vitro palmitate exposure decreased microglial mitochondrial length within 24 hours, indicating that fatty acid exposure itself is sufficient to trigger mitochondrial fission in a cell autonomous manner (Fig 2G upper panels).Collectively, these responses were independent of any detectable mitoSOX-activating ROS release (Fig S2C), and, were associated with recruitment of the mitochondrial fission regulator DRP1 (Fig 2H).A 24h exposure to the mono-unsaturated fatty acid oleate failed to elicit a comparable fission response (Fig 2G), and, acute palmitate exposure had no effects on microglial cytokine release in vitro (IL6, IL1β, TNFa; Fig S2E).Thus, acute fatty acid exposure recapitulates aMMR in vitro including selective loss of complex II activity and lack of significant inflammatory cytokine response.
C-palmitate for 4 hours, washed and subjected to GCMS or LCMS-based metabolomics (Fig 4B).Focusing first on the control microglia we observed significant palmitate uptake and incorporation into downstream metabolites (Fig 4C-F; and Fig 4O).This is the first definitive demonstration to our knowledge that microglia metabolize fatty acids. 13C-palmitate label was processed to palmitoylcarnitine and acetylcarnitine indicating that microglial fatty acid metabolism acts via the canonical CPT1/CPT2 pathway, moving carbons from outside the mitochondria into the inner mitochondrial matrix (Fig 4C, suppl Fig S4A). 13Clabelling was also found in 13 C-acetylserine, indicating that palmitate is processed through β-oxidation and that it contributes to the acetyl-coA pool (Fig 4D, suppl Fig S4B).Label was detected in all measured TCA cycle intermediates (Fig 4E, suppl Fig S4C).Examination of labelling ratios of TCA intermediates and glutamate indicated that microglia push a substantial fraction of palmitate-derived carbons out of the TCA (alpha-ketoglutarate (a-KG) to glutamate) rather than processing them further through the whole TCA cycle (towards malate) (Fig 4E-F, suppl Fig S4D) Fig 4C-D, suppl Fig S4A-B).The pre-exposure enhanced m+2 label enrichment at a-KG and glutamate without impacting other TCA intermediates (Fig 4E-F, suppl Fig S4C-D)

FIGURE 2 :
FIGURE 2: A rapid Microglial Mitochondria Response to high fat dietA.Facs plots depicting the ratio Mitotraker Deep Red/ Mitotraker Green from sorted microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (3-day HFD).B. Graph showing the ratio Mitotraker Deep Red/ Mitotraker Green from sorted hypothalamic microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 12 hours,3 days or 1-4 weeks (n=5 to 12). C. Graph showing the Mitotraker Green fluorescence from sorted hypothalamic microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for for 12 hours,3 days or 1-4 weeks (n=5 to 12).D. Volcano plot showing the metabolites content of cerebrospinal fluid from of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (n=10).E. Seahorse (+/-succinate added in the media during the experiment) on primary microglia challenged for 24hours with BSA (control) or Palmitate (experiment replicated 3times).F. Mitochondrial Electron transport chain activity recorded with FACS after TMRM staining from sorted microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (n=5).G. Mitochondrial networks from primary microglia stained with Mitotraker green after being challenged for 24hours with BSA (control), Palmitate, Oleate or LPS (n=40) and the mitochondrial length quantification graphs.H. DRP1 colocalization with the mitochondrial network stained with TOMM20 on primary microglial cell after being challenged for 24hours with BSA (control) and Palmitate(n=40) and the colocalization quantification graphs.

FIGURE 3 :
FIGURE 3: aMMR is required for diet induced homeostatic rewiring in vivo A. Immunostaining of TOMM20 and DRP1 on sorted microglia from mice Drp1 MGWT or Drp1 MGKO B. Western Blot against DRP1 on sorted microglia from mice Drp1 MGWT or Drp1 MGKO C. Graphs showing the overnight fasted glycemia, the 2hours fasted glycemia and the insulin released from Drp1 MGWT or Drp1 MGKO fed with control diet (n=5 to 11).D. Graphs showing the overnight fasted glycemia, the 2hours fasted glycemia and the insulin released from Drp1 MGWT or Drp1 MGKO fed with 3 day high fat diet (n=5 to 11).Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc test.

FIGURE 5 :
FIGURE 5: Acute HFD induces widespread MMR and rapid modulation of spatial and learning memoryA.Primary microglial cell media was collected after the13 C-glucose tracing (containing13 C-lactate released by microglia challenged with BSA or Palmitate) and incubated for 4 hours with primary neurons, the graph shows the 13 C-lactate incorporation in the neurons in relative abondance, as control, primary neurons were incubated directly with13 C-glucose (n=6).B. Graph showing the ratio Mitotraker Deep Red/ Mitotraker Green from sorted cortical microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (3-day HFD) (n=5 to 12). C. Graph showing the ratio Mitotraker Deep Red/ Mitotraker Green from sorted hippocampic microglial cells of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (3-day HFD) (n=5 to 12).D. Graph showing the latency during the Barnes Test from mice fed with normal diet (Control) or 3 days HFD (3-day HFD) (n=11).The test was performed in the VAI animals facility (USA).E. Graph showing the alternation during the T Maze Test from mice fed with normal diet (Control) or 3 days HFD (3-day HFD) (n=11).The test was performed in the VAI animals facility (USA).F. Graph showing the distance walked during the ROTAROD test from mice fed with normal diet (Control or 3 days HFD (3-day HFD) (n=11).G. Graph showing the number of turn before the mice fall during the ROTAROD test from mice fed with normal diet (Control) or 3 days HFD (3-day HFD) (n=11).H. Graph showing the latency during the ROTAROD test from mice fed with normal diet (Control) or 3 days HFD (3-day HFD) (n=11).I. Microglial staining with Iba1 (green) in the Hippocampus slices from mice fed with 3 days HFD or mice depleted from their microglial cells with 1 week control diet complexed with PLX-5662 prior the 3 days HFD (PLX-5662) (n=5).J. Graph showing the latency during the Barnes Test from mice fed with normal diet (Control) or with depleted microglia (n=8).K. Graph showing the latency during the Barnes Test from Drp1 MGWT or Drp1 MGKO mice fed with normal diet (Control diet ) or with 3 days HFD (3-day HFD) (n=11).Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc test.

SUPPL FIGURE 1 :SUPPL FIGURE 3 :SUPPL FIGURE 4 :
Acute HFD-induced metabolic changes are microglia dependent A. Graphs showing the Glucose Tolerance Test (OGTT) and the associated-insulin kinetic of C57Bl6/J male before keeping them with a control diet (Control) or before feeding them with high fat diet for 3 days (3-day HFD) (n=8).B. Graph showing the Insulin Tolerance Test (ITT) of C57Bl6/J male before keeping them with a control diet (Control) or before feeding them with high fat diet for 3 days (3-day HFD) (n=5).C. Graphs showing the Glucose Tolerance Test (OGTT) expressed in percentage from the basal glycemia of C57Bl6/J male fed with a control diet (Control) or fed with high fat diet for 3 days (3day HFD) (n=8).D. Graph showing the body weight evolution between C57Bl6/J male fed with control diet (Control) or fed with high fat diet for 3 days (3-day HFD), before and after the diet change.(n=7).E. IBA1 immunostaining on hypothalamic slices from C57Bl6/J male fed with control diet (Control) or fed with high fat diet for 3 days (3-day HFD) and its quantification (n=10).F. IBA1 and YFP immunostaining on hypothalamic slices from Cx3cr1creERT2-Rosa26YFP mice fed with control diet or 3 days high fat diet for 3 days (n=5).G. sgRNAseq dataset from hypothalamic microglia cells harvested from C57bl6/J male mice fed with control diet (Control) and High Fat Diet for 3 days (HFD_3d) (n=5) Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc.SUPPL FIGURE2: A rapid Microglial Mitochondria Response to high fat diet A. IBA1 immunostaining of primary microglia cells treated with BSA (control) or Palmitate for 24hours.B. Graph showing mRNA expression of microglial marker (TMEM119) or astrocytes marker (S100B) or macrophages marker (Arg1) in primary microglial culture challenged for 24hours with BSA or palmitate.Primary culture of astrocytes and macrophages were used as positive controls.C. MitoSOX staining of primary microglial cells after being challenged for 24hours with BSA (control), Palmitate, Oleate or LPS (n=10) and the MitoSOX quantification graph.D. Mitochondrial networks from primary microglia stained with Mitotraker green and stained with MitoSOX after being challenged for 2hours with BSA (control), Palmitate, Oleate or LPS (n=40) and the mitochondrial length as well as the MitoSOX quantification graphs F. Interleukins concentrations (TNFalpha, IL-1beta, IL6) in the primary microglia cells media after being challenged for 24hours with BSA or palmitate (n=7) Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc.aMMR is required for diet induced homeostatic rewiring in vivo A Schematic depicting the timeline for the tamoxifen injection and the experiments performed on Drp1 MGWT or Drp1 MGKO B. Graph showing the body weight evolution among all the Drp1 MGKO genotypes after the tamoxifen injection (n=6 to 11) C. Graphs showing the fat mass and lean mass evolution among all the Drp1 MGKO genotypes after the tamoxifen injection (n=6 to 11) D. Graphs showing the body weight before and after the 3 day HFD for the mice Drp1 MGWT and Drp1 MGKO (n=11 to 13) Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc.Palmitate induces a novel microglial lactate/succinate/itaconate release pathway.

SUPPL FIGURE 5 :
The results are graphed in pool size.Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc.Acute HFD induces widespread MMR and rapid modulation of spatial and learning memory A. Map2, NeuN, Iba1 and Map2, NeuN, GFAP immunostainings on primary neurons.B. Primary microglial cell media was collected after the 13 C-glucose tracing (containing13 Cmetabolites released by microglia challenged with BSA or Palmitate) and incubated for 4 hours with primary neurons, the graph shows the 13 C-citrate,13 C-itaconate and 13 C-succinate incorporation in the neurons in relative abondance (n=6).C. Graph showing the latency during the Barnes Test from mice fed with normal diet (Control), or 3 days HFD (3-day HFD) (n=11).The test was performed in the MPI animals facility (Germany).D. Graph showing the alternation during the T Maze Test from mice fed with normal diet (Control), or 3 days HFD (3-day HFD) (n=11).The test was performed in the MPI animals facility (Germany).E. Graph showing the distance walked during the ROTAROD test from mice fed with normal diet (Control) or depleted from their microglia (n=8).

F.
Graph showing the number of turns before the mice fall during the ROTAROD test from mice fed with normal diet (Control), (Control) or depleted from their microglia (n=8).G. Graph showing the latency during the ROTAROD test from mice fed with normal diet (Control) or depleted from their microglia (n=8).H. Graph showing the distance walked during the ROTAROD test from Drp1 MGWT or Drp1 MGKO mice fed with normal diet (Control diet), or 3 days HFD (3-day HFD) (n=11).I. Graph showing the number of turns before the mice fall during the ROTAROD test from Drp1 MGWT or Drp1 MGKO mice fed with normal diet (Control diet), or 3 days HFD (3-day HFD) (n=11).I. Graph showing the latency during the ROTAROD test from Drp1 MGWT or Drp1 MGKO mice fed with normal diet (Control diet), or 3 days HFD (3-day HFD) (n=11).Data are presented as mean ±SEM.*p<0.05,**p<0.01,***p<0.001as determined by two-tailed Student's test and two-way ANOVA followed by Bonferroni post hoc.

Fig 4K-N). Palmitate pretreatment increased all of these signatures, including glucose uptake (Fig 4J), lactate labelling and lactate release. aMMR is therefore characterized by induction of a Warburg-like metabolic signature (Fig 4N). Interestingly, as with palmitate-tracing experiments, fatty acid pretreatment increased release of tracer-labeled itaconate and succinate into the extracellular media (Fig 4L-M).
C-glucose incorporation into lactate (m+3) released during the 6 hours tracing experiment by primary microglia pretreated for 24hours with BSA or palmitate (n=3).