PMC

Photokinetic effects in the mSMR curves of DNA measurements. A Division of the mSMR curves by the diffusive part of the mSMR model. B The mean proportions F with standard deviations from the isomerization term for the DNA mixtures

Characterization of P_msmR. (A) The DNA sequence between msmR and ytaP start codons is shown. The open arrows show the start codons of ytaP and msmR. The core elements of P_msmR (−35 and −10 boxes) and the transcription start site of msmR are indicated with boxes. The protected regions of P_msmR DNA by MsmR from DNase I digestion (FP1 and FP2) are in red letters. The inverted repeats within the MsmR binding site are indicated by solid arrows. The putative cre site (gray highlighted) is also demonstrated. The green boxes show the DNA regions used for the electrophoretic mobility shift assay. (B) Identification of the transcription start site (TSS) of msmR was performed by primer extension. The migration of the generated cDNA fragment (orange) was compared with the sequencing reaction. (C) An electrophoretic mobility shift assay was carried out using 5′-end Cy5-labeled DNA fragments of P_msmR, the FP1 inverted repeat, and the FP2 inverted repeat. The amplified DNA fragment from the GlcR binding site was used as a negative control. The migration of the DNA fragment was investigated in the absence (−) or presence (+) of MsmR. (D) The chromatographs of the DNA footprinting and DNA sequencing reactions are separately shown. The 6-FAM-labeled P_msmR DNA was digested with DNase I in the absence (orange) or presence (blue) of 0.27 mM MsmR. The identified DNA footprints, FP1 and FP2, were then compared with the DNA sequencing reaction utilizing ddATP (green), ddGTP (black), ddCTP (blue), and ddTTP (red).

Relations between sperm dimensions and mass-specific metabolic rate (MSMR) in eutherian mammals. (a) Maximum total sperm length; (b) total sperm length range. Each point represents one of six MSMR categories. MSMR values are log₁₀ transformed.

Number of diagnosed cases of coccidioidomycosis between 2000 and 2017 among active military. Data were compiled from two studies: Medical Surveillance Monthly Report (MSMR) 2010 Vol. 17(12):13 [], and MSMR 2018 Vol. 25(4):2–5 [] (A). Incidence of coccidioidomycosis within the active component, U.S. Armed Forces, 2000–2013 per 100,000 persons/year in the southwestern U.S. (adopted from MSMR 2014, Vol. 21(16):12–14 []) (B).