PMC

Tail-cuff systolic blood pressure in alpha-lipoic acid prevention studies. (a) DEX-HT, (b) ACTH-HT. ■ saline, n = 10; ▲ DEX, n = 10; □ ACTH, n = 10; ▼ alpha-lipoic acid + saline, n = 10; ♦ alpha-lipoic acid + DEX, n = 10, ◊ alpha-lipoic acid + ACTH, n = 10, ● high dose alpha-lipoic acid + ACTH, n = 4, data were presented as mean ± SEM.

Variation of the contraction rate of the samples as a function of the air exposure 3, 5, and 7 days with the different concentrations (0, 1, 10 µM) of α-lipoic acid. The graph is the contraction rate of the group treated with α-lipoic acid for (a) 3 days, (b) 5 days, and (c) 7 days under air exposure culture conditions. The legends in the graphs (a–c) are the same. (■, 0 µM α-lipoic acid; ●, 1 µM α-lipoic acid; and ▲, 10 µM α-lipoic acid) (d) It is a graph comparing only the contraction rate of the 8th day, which is the period of coculture to dermis and epidermis, and the air exposure period of which α-lipoic acid was treated (maximum processing period for α-lipoic acid is 7 days). The change was represented by a linear curve during the α-lipoic acid processing period. (e) Photographic images of the samples following air exposure for the different concentrations of α-lipoic acid (bar = 8 mm).

Radioprotective effect of Q10, resveratrol, alpha-lipoic acid, and combination of resveratrol and alpha-lipoic acid on mice spermatogenesis. A and B: normal spermatogenesis; C: reduction of spermatogonia cells; D: spermatogenic arrest in epididymis; E: Radiation + Q10; F: Radiation + Resveratrol; G: Radiation + Alpha-lipoic acid; and H: Radiation + Resveratrol + Alpha-lipoic acid. Irradiation caused significant reduction of spermatogonia numbers, while treatment with Q10, resveratrol, alpha-lipoic acid, or combination of resveratrol and alpha-lipoic acid was able to reduce radiation suppressive effect (H&E staining, Nikon E400 Camera DSFi1, ×40 magnification for A, C, E, F, G, and H, and ×400 magnification for B and D).

Representative chromatograms for α-lipoic acid chiral separation in plasma. Chromatograms shown with solid and dotted line are for α-lipoic acid and α-lipoic acid-d5 (internal standard), respectively. Blank plasma (A); blank plasma spiked with α-lipoic acid (5 ng of each enantiomer/mL, B); and blank plasma spiked with α-lipoic acid (1250 ng of each enantiomer/mL, C).