PMC

SIR chromatograms of polymyxin B1 and polymyxin B2; Figure A = calibrator with polymyxin B1 plasma concentration of 2500 ng/mL; Figure B = calibrator with polymyxin B2 plasma concentration of 2500 ng/mL; Figure C = calibrator with polymyxin B1 plasma concentration of 100 ng/mL; Figure D = calibrator with polymyxin B2 plasma concentration of 100 ng/mL; Figure E = Patient sample with polymyxin B1 plasma concentration of 615 ng/mL; Figure F = Patient sample with polymyxin B2 plasma concentration of 322 ng/mL.

Calibration curves for polymyxin B1 (A) and polymyxin B2 (B).

Cell surface morphologies of Xoo treated with polymyxin B₁ and E₂. (A) CK, (B) Xoo cells treated with polymyxin B₁ (1 μg/mL), (C) Xoo cells treated with polymyxin B₁ (5 μg/mL), (D) Xoo cells treated with polymyxin B₁ (10 μg/mL), (E) Xoo cells treated with polymyxin E₂ (1 μg/mL), (F) Xoo cells treated with polymyxin E₂ (5 μg/mL), (G) Xoo cells treated with polymyxin E₂ (10 μg/mL).

Molecular models of Kir4.2 channel with polymyxin B₁. A The Kir4.2 channel model is shown in NewCartoon presentation (subunits are in yellow, blue, grey and purple). B Binding of polymyxin B₁ to the Kir4.2 channel. Polymyxin B₁ are shown in green, and the binding amino acids are in red. C Gate distance of the channel in the inactivate state (orange) and the state after polymyxin B₁ bound. Purple balls represent K⁺ atoms. Independent simulations were conducted three times