PMC

Pantoprazole induced angiogenic factors related to HO-1 induction. (A) RT-PCR and western blot for VEGF. Significant inductions of VEGF were noted with pantoprazole, significantly after 300 μM pantoprazole (p<0.05). These figures are representatives of the results obtained in 3 independent experiments. (B) The changes of VEGF and HO-1 with pantoprazole alone or combination of pantoprazole and ZnPPIX. The induction of VEGF after pantoprazole was significantly attenuated with HO-1 inhibitor, signifying the implication of HO-1 in PPI-induced VEGF. These figures are representatives of the results obtained in 3 independent experiments. (C) Other angiogenic factors related to pantoprazole. bFGF and VEGF was increasingly expressed with pantoprazole, but these inductions were attenuated with HO-1 inhibitor. These figures are representatives of the results obtained in 3 independent experiments. (D)in vitro angiogenesis assay. Tube formation of HUVEC was significantly decreased with 50 μM indomethacin. Pantoprazole compensated indomethacin-induced defective angiogenesis as assessed with the percentage of tube formation, whereas HO-1 inhibitor cancelled these overcome of pantoprazole-induced angiogenesis. These figures are representatives of the results obtained in 3 independent experiments.

Pantoprazole regulates the extra- and intracellular pH of cancer cells. (A) Change in the extracellular pH of the cell culture medium of prostate (PC3, DU145), breast (MCF7) and ovarian cancer (SKOV3) cells following treatment with pantoprazole (grey columns) or without pantoprazole (controls, white columns) at a pH of 6.5 or 7.5. (B) Change in the intracellular pH of prostate (PC3 and DU145), breast (MCF7) and ovarian cancer (SKOV3) cells following treatment with or without 100 μM pantoprazole for 6 h at a pH of 6.5 or 7.5. Top panel: cells were incubated in cell culture medium of different pHs; middle panel: cells were incubated in cell culture medium with a pH of 6.5 with or without pantoprazole; bottom panel: cells were incubated in cell culture medium with a pH 7.5 with or without pantoprazole. Red pick: unstained cells; pink pick: cells incubated in cell culture medium with a pH of 7.5 containing pantoprazole; green pick: cells incubated in cell culture medium with a pH of 7.5 without pantoprazole; X-axis: FITC; Y-axis: cell count. The bars represent the mean and SD of the mean of n≥3.

Apoptosis of K562 cells (a) and K562/A02 cells (b) and K562/ADM (c) cells pretreated with different concentration of pantoprazole. 1 Control; 2 50 µg/ml of pantoprazole; 3 100 µg/ml of pantoprazole; 4 200 µg/ml of pantoprazole; 5 50 µg/ml of pantoprazole + 1 µg/ml of amycin

Cellular cycle of K562 cells (a) and K562/A02 cells (b) and K562/ADM (c) cells pretreated with different concentration of pantoprazole. 1 Control; 2 50 µg/ml of pantoprazole; 3 100 µg/ml of pantoprazole; 4 200 µg/ml of pantoprazole; 5 50 µg/ml of pantoprazole + 1 µg/ml of amycin