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Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring Kcat for p-nitroanilide release at 0.1 uM using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay (Rvb = 1.8 +/- 0.03 /s)
Assay data:5 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as reduction in aminopeptidase activity by measuring p-nitroanilide release using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Activation of N-terminal (His)6 and Xpress-tagged LTA4H (unknown origin) expressed in Escherichia coli BL21(DE3) cells assessed as increase in aminopeptidase activity by measuring p-nitroanilide release using L-Alanine-p-nitroanilide incubated for 10 mins by UV plate reader based assay
Assay data:8 Active, 3 Activity ≤ 1 µM, 9 Tested
Inhibition of (His)6 tagged human recombinant LTA4H expressed in Escherichia coli BL21(DE3) using non-fluorescent L-arginine-7-amino-4-methylcoumarine as substrate preincubated for 30 mins followed by substrate addition and measured for 30 mins by fluorescence based assay
Assay data:10 Active, 8 Activity ≤ 1 µM, 17 Tested
Inhibition of human recombinant LTA4H by L-(4-benzoyl)phenylalanyl-beta-naphthylamide fluorigenic substrate by fluorescence based assay
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Inhibition of LTA4H (unknown origin) at 500 uM preincubated for 15 mins followed by LTA4 substrate addition and measured after 10 mins by ELISA relative to control
Assay data:1 Tested
Binding affinity to recombinant human LTA4H hydrolase assessed as change in melting temperature at 10 uM by SYPRO orange dye-based thermofluor assay
Inhibition of recombinant human LTA4H Epoxide Hydrolase expressed in Escherichia coli BL21 (DE3) pLysS at 10 uM preincubated for 10 mins followed by addition of LTA4 as substrate measured after 15 mins by reverse-phase HPLC analysis
Assay data:18 Tested
Inhibition of recombinant human LTA4H aminopeptidase activity expressed in Escherichia coli BL21 (DE3) pLysS assessed as formation of p-NA from Ala-p-NA at 10 uM preincubated for 10 mins followed by substrate addition measured after 10 mins
Binding affinity to recombinant human LTA4H hydrolase assessed as change in melting temperature at 50 uM by SYPRO orange dye-based thermofluor assay
Inhibition of recombinant human LTA4H Epoxide Hydrolase expressed in Escherichia coli BL21 (DE3) pLysS preincubated for 10 mins followed by addition of LTA4 as substrate measured after 15 mins by reverse-phase HPLC analysis
Assay data:2 Active, 1 Activity ≤ 1 µM, 18 Tested
Inhibition of recombinant human LTA4H aminopeptidase activity expressed in Escherichia coli BL21 (DE3) pLysS assessed as formation of p-NA from Ala-p-NA preincubated for 10 mins followed by substrate addition measured after 10 mins
Assay data:3 Active, 1 Activity ≤ 1 µM, 18 Tested
Inhibition of full-length recombinant C-terminal FLAG-tagged human leukotriene A4 hydrolase expressed in baculovirus-infected insect cells using Arg-AMC as substrate after 1 hr by fluorescence assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Summary
Inhibition of recombinant C-terminal His-tagged human LTA4H epoxide hydrolase activity expressed in Escherichia coli assessed as reduction in LTB4 production using LTA4 as substrate preincubated for 20 mins followed by substrate addition measured after 30 mins by enzyme immunoassay
Assay data:1 Active, 19 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant C-terminal His-tagged human LTA4H aminopeptidase activity expressed in Escherichia coli using L-alanine-4-nitro-anilide hydrochloride as substrate preincubated for 20 mins followed by substrate addition
Assay data:4 Active, 19 Tested
Non-competitive inhibition of human C-terminal his6-tagged/N-terminal T7 gene leader sequence-tagged LTA4H using varying levels of L-arginine-7-amino-4-Methylcoumarine as substrate preincubated for 30 mins followed by substrate addition measured for 30 mins by fluorescence assay
Binding affinity to human C-terminal His6-tagged/N-terminal T7 gene leader sequence-tagged LTA4H assessed as change in melting temperature at 0.5 mM by circular dichroism spectroscopic analysis
Assay data:2 Tested
Binding affinity to human C-terminal His6-tagged/N-terminal T7 gene leader sequence-tagged LTA4H assessed as change in melting temperature at 1 mM by circular dichroism spectroscopic analysis
Binding affinity to human C-terminal his6-tagged/N-terminal T7 gene leader sequence-tagged LTA4H at 35 degC by ITC method
Assay data:5 Active, 3 Activity ≤ 1 µM, 5 Tested
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