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Nitric Oxide Synthase, Inducible (iNOS) Eurofins-Panlabs enzyme assay
Assay data:1 Tested
SummaryRelated BioAssays by Target
Assay data:4 Tested
Summary
Inhibition of N-terminal His-tagged human recombinant iNOS expressed in Escherichia coli assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate incubated for 30 min by ELISA
Assay data:8 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal His-tagged human recombinant iNOS expressed in Escherichia coli assessed as reduction in L-[3H]-citrulline level using L-[3H]-arginine as substrate at 1 mM incubated for 30 mins by liquid scintillation method relative to control
Assay data:19 Tested
Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli at 1 uM using L-arginine as substrate preincubated for 15 mins followed by NADPH addition and measured after 20 mins by OPA/NAC reagent based HPLC-fluorimetric analysis relative to control
Assay data:11 Tested
Inhibition of iNOS (unknown origin)
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of iNOS (unknown origin) at 20 uM relative to control
Assay data:3 Tested
Inhibition of human iNOS expressed in Escherichia coli using L-arginine as substrate in presence of human oxyhemoglobin after 6 mins by hemoglobin NO capture assay
Assay data:7 Active, 7 Tested
Inhibition of iNOS (unknown origin) assessed as formation of methemoglobin from oxyhemoglobin at 1 mM using L-arginine as substrate in presence of oxyhemoglobin and NADPH by UV-vis spectrophotometry relative to control
Assay data:16 Tested
Inhibition of human iNOS expressed in Escherichia coli expression system assessed as reduction in NO production in presence of L-arginine measured for 30 sec by hemoglobin capture assay
Assay data:2 Active, 8 Tested
Inhibition of LPS-induced iNOS in human BV2 cells after 4 hrs by DAF-FMDA dye-based fluorescence assay
Assay data:4 Active, 6 Tested
Inhibition of iNOS (unknown origin) at 1 uM
Human Inducible NOS (Nitric oxide synthases)
Assay data:2 Active, 2 Tested
Inhibition of recombinant human IL-1beta-induced iNOS in human cartilage explants assessed as inhibition of nitrite production incubated for 18 to 24 hrs followed by IL-1beta and compound addition and further incubated for 20 to 24 hrs by fluorometric assay
Assay data:3 Active, 2 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of human iNOS expressed in Sf9 insect cells assessed as reduction in conversion of L-[2,3-3H]-arginine to L-[2,3-3H]-citrulline incubated for 15 mins by liquid scintillation counting method
Assay data:5 Active, 1 Activity ≤ 1 µM, 9 Tested
Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli assessed as L-[3H]-citrulline formation using L-[3H]-arginine as substrate after 30 mins by liquid scintillation spectroscopy
Assay data:6 Tested
Inhibition of recombinant N-terminal His-tagged human iNOS expressed in Escherichia coli assessed as L-[3H]-citrulline formation at 1 mM using L-[3H]-arginine as substrate after 30 mins by liquid scintillation spectroscopy
Assay data:31 Tested
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