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Selectivity interaction (Reader proteins panel (DSF assay)) EUB0000034d UHRF1
Assay data:1 Tested
SummaryRelated BioAssays by Target
AlphaScreen Assay from Article 10.1021/cb500956g: "Identification of a fragment-like small molecule ligand for the methyl-lysine binding protein, 53BP1."
Assay data:25 Tested
SummaryPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Binding affinity to UHRF1 TTD (unknown origin) assessed as increase in thermal stability by melting temperature by SYPRO orange dye based fluorescence assay (Rvb = 46.5 +/- 0.1 degreeC)
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to UHRF1 F278A mutant (unknown origin) assessed as dissociation constant by ITC analysis
Binding affinity to UHRF1 M224A mutant (unknown origin) assessed as dissociation constant by ITC analysis
Binding affinity to UHRF1 TTD (unknown origin) assessed as compound-protein complex stoichiometry ratio of 1:1 by ITC analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of UHRF1 TTD binding with LIG1K126me3 in Xenopus laevis eggs incubated for 1 hr by immunoprecipitation
Displacement of FAM-labeled LIG1K126me3 from UHRF1 TTD(unknown origin) by fluorescence polarization assay
Displacement of FAM-labeled H3K9me3 from UHRF1 TTD(unknown origin) by fluorescence polarization assay
Binding affinity to UHRF1 TTD variant loop (unknown origin) assessed as dissociation constant by ITC analysis
Binding affinity to UHRF1 Y188A/Y191A double mutant (unknown origin) assessed as dissociation constant by ITC analysis
Binding affinity to UHRF1 TTD (unknown origin) assessed as change in enthalpy by ITC analysis
Binding affinity to UHRF1 TTD (unknown origin) assessed as dissociation constant by ITC analysis
Binding affinity to UHRF1 TTD (unknown origin) assessed as increase in thermal stability by measuring change in melting temperature by SYPRO orange dye based fluorescence assay
Binding affinity to UHRF1 (unknown origin) assessed as induction of thermal shifts at 200 uM measured for 25 mins by SYPRO orange dye thermal shift assay
Binding affinity to 6His-tagged and human UHRF1 tandem TUDOR domain (126 to 285 residues) expressed in Escherichia coli BL21(DE3)-T1R by ITC method
Binding affinity to UHRF1 (unknown origin) at 200 uM by DSF assay
Inhibition of UHRF1 in human MCF7 cells assessed as reduction in global 5-methylcytosine level at 15 uM after 72 hrs by HPLC-MS/MS analysis relative to control
Assay data:2 Tested
Inhibition of UHRF1/DNMT1 protein interaction in human U251 cells assessed as reduction in 5-methylcytosine level at 15 uM after 72 hrs by ELISA relative to control
Inhibition of UHRF1/DNMT1 protein interaction in human U251 cells at 15 uM after 4 hrs by DAPI staining based P-LISA
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