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Selectivity interaction (Eurofins-Panlabs screen) EUB0000542a ALOX5
Assay data:1 Tested
SummaryRelated BioAssays by Target
Selectivity interaction (MSD Pharma/Taiwan screen (enzymes and receptors) ) EUB0000296b ALOX5
Inhibition of human recombinant 5-LOX incubated for 5 mins by fluorimetry
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of 5-LOX in human neutrophils assessed as reduction in FLAP-dependent 5-LO product LTB4 formation at upto 10 uM preincubated for 10 mins followed by A23187 addition and measured after 10 mins by RP-HPLC and UV detection method
Assay data:3 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of 5-LOX in human neutrophils assessed as reduction in FLAP-dependent 5-LO product LTB4 formation at upto 1 uM preincubated for 10 mins followed by A23187 addition and measured after 10 mins by RP-HPLC and UV detection method
Assay data:3 Tested
Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21 assessed as inhibition of FLAP-dependent 5-LO product LTB4 formation preincubated for 15 mins followed by arachidonic acid addition and measured after 10 mins by RP-HPLC analysis
Inhibition of 5-LO in human leucocytes by measuring FLAP-dependent 5-LOproduct LTB4 in presence of calcium ionophore A23187
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human LOX-5 using arachidonic acid and ATP as substrate preincubated for 10 mins followed by substrate addition and measured after 20 mins by fluorescent assay
Inhibition of human LOX-5 in presence of LOX substrate solution incubated for 10 mins by Varioskan-flash microplate spectrophotometer reader
Assay data:8 Tested
Inhibition of 5-LOX (unknown origin) using arachidonic acid as substrate at 100 uM preincubated for 5 mins followed by substrate addition measured after 15 mins by absorbance based analysis relative to control
Inhibition of 5-LOX in human PMNL assessed as enzyme residual activity at 3 uM preincubated for 15 mins followed by A23187 addition and measured after 10 mins by RP-HPLC analysis
Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21(DE3) assessed as enzyme residual activity at 3 uM using arachidonic acid as substrate preincubated with enzyme for 15 mins followed by substrate addition and measured after 10 mins by RP-HPLC analysis
Inhibition of 5-LOX in human PMNL assessed as reduction in all-trans isomers of LTB4 and 5-HETE formation preincubated for 15 mins followed by A23187 addition and measured after 10 mins by RP-HPLC analysis
Assay data:7 Active, 2 Activity ≤ 1 µM, 27 Tested
Inhibition of human recombinant 5-LOX expressed in Escherichia coli BL21(DE3) assessed as reduction in all-trans isomers of LTB4 and 5-HETE formation using arachidonic acid as substrate preincubated with enzyme for 15 mins followed by substrate addition and measured after 10 mins by RP-HPLC analysis
Assay data:13 Active, 7 Activity ≤ 1 µM, 21 Tested
Inhibition of human recombinant LOX-5 using arachidonic acid and ATP as substrate preincubated for 10 mins followed by substrate addition and measured after 20 mins by fluorescent assay
Inhibition of 5-LO in thapsigargin stimulated human PMNL cells assessed as reduction in 5-LO product level preincubated for 5 mins followed by thapsigargin addition and measured after 15 mins by RP-HPLC analysis
Assay data:22 Active, 19 Activity ≤ 1 µM, 22 Tested
Inhibition of 5-LO in A23187-stimulated human HEK293 cells assessed as reduction in 5-LO product level at 1 uM preincubated for 5 mins followed by A23187, arachidonic acid addition and measured after 15 mins by RP-HPLC analysis relative to control
Assay data:26 Active, 36 Tested
Inhibition of human recombinant LOX-5 preincubated for 10 mins in presence of AA and ATP measured after 20 mins by fluorescent assay
Assay data:4 Tested
In Vitro Lipoxygenase (LOX) Inhibitory Assay from Article 10.1016/j.bioorg.2017.03.012: "New hybrid molecules combining benzothiophene or benzofuran with rhodanine as dual COX-1/2 and 5-LOX inhibitors: Synthesis, biological evaluation and docking study."
Assay data:15 Active, 16 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
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