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HSP90 Competition Binding (Fluorescence Polarization) Assay from US Patent US11560377: "Fused amino pyridine as HSP90 inhibitors"
Assay data:54 Active, 39 Activity ≤ 1 µM, 62 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition of human recombinant Hsp90beta expressed in Escherichia coli BL21(DE3) incubated for 24 hrs by fluorescence polarization assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human recombinant Hsp90alpha (1 to 223 residues) expressed in Escherichia coli BL21(DE3) incubated for 24 hrs by fluorescence polarization assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to recombinant human Hsp90beta incubated for 30 mins by intrinsic fluorescence Spectroscopy
Binding affinity to recombinant human Hsp90alpha incubated for 30 mins by intrinsic fluorescence Spectroscopy
Binding affinity to recombinant human Hsp90beta assessed as inhibition constant incubated for 2 hrs by fluorescence polarization assay
Binding affinity to recombinant human Hsp90alpha assessed as inhibition constant incubated for 2 hrs by fluorescence polarization assay
Binding affinity to human Hsp90alpha expressed in Escherichia coli BL21(DE3) incubated for 5 hrs by fluorescence polarization assay
Inhibition of human N-terminal His-tagged Hsp90alpha (1 to 236 residues) expressed in Escherichia coli BL21(DE3) measured after 24 hrs by fluorescence polarization assay
Inhibition of human cy3B-GM labeled Hsp90beta expressed in Escherichia coli BL21(DE3) measured after 24 hrs by fluorescence polarization assay
Inhibition of human cy3B-GM labeled Hsp90alpha expressed in Escherichia coli BL21(DE3) measured after 24 hrs by fluorescence polarization assay
Binding affinity to Hsp90alpha (unknown origin) by fluorescence polarization assay
Binding affinity to human Hsp90 expressed in Escherichia coli by SPR assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 6 Tested
Binding affinity to Hsp90 (unknown origin) at 80 uM by UV-Vis spectrophotometric analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Degradation of HSP90alpha in human MCF7 cells at 2 uM measured after 6 hrs by western blot analysis
Binding affinity to HSP90 (unknown origin) upto 250 uM by BLI assay
Inhibition of HSP90 chaperone activity in human HeLa cell lysate assessed as suppression of denatured recombinant firefly luciferase refolding at 100 uM measured upto 60 mins by luminescence based microplate reader
Assay data:2 Active, 2 Tested
Inhibition of HSP90 in human HT-1080 cells assessed as decrease in CDK4 protein level by measuring half-life of CDK4 protein at 20 uM measured upto 24 hrs in the presence of cycloheximide by Western blotting analysis
Inhibition of HSP90 in human HT-1080 cells assessed as downregulation of CDK4 protein expression measured after 24 hrs by Western blot analysis
Inhibition of HSP90 in human HT-1080 cells assessed as downregulation of Akt protein expression measured after 24 hrs by Western blot analysis
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