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CARM1 Enzyme Assay from US Patent US20230279020: "CARM1 INHIBITORS AND USES THEREOF"
Assay data:1028 Active, 988 Activity ≤ 1 µM, 1030 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Selectivity interaction (ITC) EUB0000103c CARM1
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Selectivity interaction (DSF assay) EUB0000103c CARM1
Assay data:1 Tested
SummaryRelated BioAssays by Target
Selectivity interaction (HMT panel) EUB0000194b CARM1
Affinity Biochemical interaction (Biochemical assay (measuring transfer of the methyl group from 3H-SAM to the peptide substrates)) EUB0000103c CARM1
Affinity Biochemical interaction (Scintillation proximity assay (SPA) ) EUB0000034d CARM1
Affinity On-target Cellular interaction (Western Blot (PRMT4 dependent cellular asymmetric arginine dimethylation of Med12 (Med12-Rme2a) in HEK293 cells)) EUB0000103c CARM1
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of PRMT4 (unknown origin) using histone H4 (1 to 21 residues) peptide/SAM as substrate incubated for 210 mins in presence of SAM by AlphaLISA assay
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human PRMT4 at 10 uM by radioisotope-based filter binding assay
Inhibition of human PRMT4 activity in human MCF7 cells assessed as reduction in arginine methylation at 10 to 50 uM
Inhibition of human PRMT4 activity in human MCF7 cells assessed as reduction in arginine methylation measured after 4 to 8 days
Inhibition of human PRMT4 activity in human HEK293T cells incubated for 24 hrs by western blot analysis
Binding affinity to full length recombinant PRMT4 (unknown origin) assessed as in vitro residence time by surface plasmon resonance analysis
Assay data:9 Tested
Binding affinity to full length recombinant PRMT4 (unknown origin) assessed as binding constant (Koff) by surface plasmon resonance analysis
Binding affinity to full length recombinant PRMT4 (unknown origin) assessed as binding constant (Kon) by surface plasmon resonance analysis
Binding affinity to full length recombinant PRMT4 (unknown origin) assessed as dissociation constant by surface plasmon resonance
Assay data:9 Active, 7 Activity ≤ 1 µM, 9 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human full length recombinant GST-tagged PRMT4 (2 to 608 residues) using histone H3 as substrate incubated for 20 mins in presence of [3H]-SAM by radioisotope-based filter assay
Assay data:7 Active, 6 Activity ≤ 1 µM, 8 Tested
Inhibition of PRMT4 (unknown origin)
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Assay data:5 Active, 1 Activity ≤ 1 µM, 6 Tested
Inhibition of N-terminal GST-tagged PRMT4 (2 to 608 residues)(unknown origin) expressed in Escherichia coli at 1 uM using 3H-SAM as a substrate relative to control
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