Alternative titles; symbols
HGNC Approved Gene Symbol: RPAP2
Cytogenetic location: 1p22.1 Genomic coordinates (GRCh38): 1:92,299,059-92,402,056 (from NCBI)
Jeronimo et al. (2007) identified RPAP2 as an RNA polymerase II (see 180660)-associated protein in HEK293 human embryonic kidney cells and obtained a full-length RPAP2 cDNA by database and sequence analyses. The deduced protein contains 612 amino acids.
Using affinity purification, mass spectrometry, and bioinformatic analysis, Jeronimo et al. (2007) mapped an interaction network of protein complexes involved in the RNA polymerase II machinery. They found that RPAP1 (611475), RPAP2, RPAP3 (611477), and XAB1 (611479) were tightly bound to RNA polymerase II in the soluble nuclear compartment in HEK293 cells. Examination of the protein interaction network revealed that the RPAPs and XAB1 also interacted with components of the Mediator (e.g., MED17; 603810) and Integrator (e.g., INTS3; 611347) complexes, as well as with proteins able to assemble into molecular chaperone complexes, including the prefoldins (e.g., PFDN6; 605660) and the AAA+ ATPases RUVBL1 (603449) and RUVBL2 (604788).
The International Radiation Hybrid Mapping Consortium mapped the RPAP2 gene to chromosome 1 (RH103075).
Jeronimo, C., Forget, D., Bouchard, A., Li, Q., Chua, G., Poitras, C., Therien, C., Bergeron, D., Bourassa, S., Greenblatt, J., Chabot, B., Poirier, G. G., Hughes, T. R., Blanchette, M., Price, D. H., Coulombe, B. Systematic analysis of the protein interaction network for the human transcription machinery reveals the identity of the 7SK capping enzyme. Molec. Cell 27: 262-274, 2007. [PubMed: 17643375] [Full Text: https://doi.org/10.1016/j.molcel.2007.06.027]