Alternative titles; symbols
HGNC Approved Gene Symbol: AP2A2
Cytogenetic location: 11p15.5 Genomic coordinates (GRCh38): 11:925,870-1,012,240 (from NCBI)
By randomly sequencing cDNA clones from size-fractionated human brain cDNA libraries, Nagase et al. (1998) identified AP2A2, which they designated KIAA0899. The deduced 929-amino acid protein shares more than 97% sequence identity with rat alpha-adaptin. By RT-PCR analysis followed by ELISA for quantitation, they detected ubiquitous, low-to-moderate expression of KIAA0899, with highest levels in spinal cord and fetal and adult brain and lowest levels in spleen.
Using the N terminus of huntingtin (HTT; 613004) in a yeast 2-hybrid screen of a testis cDNA library, Faber et al. (1998) identified AP2A2, which they designated huntingtin yeast partner J (HYPJ). The predicted protein shares significant sequence identity with mouse alpha-adaptin C.
By radiation hybrid analysis, Nagase et al. (1998) mapped the AP2A2 gene to chromosome 11.
Faber, P. W., Barnes, G. T., Srinidhi, J., Chen, J., Gusella, J. F., MacDonald, M. E. Huntingtin interacts with a family of WW domain proteins. Hum. Molec. Genet. 7: 1463-1474, 1998. [PubMed: 9700202] [Full Text: https://doi.org/10.1093/hmg/7.9.1463]
Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 355-364, 1998. [PubMed: 10048485] [Full Text: https://doi.org/10.1093/dnares/5.6.355]