Entry - *607074 - PROTEIN KINASE D2; PRKD2 - OMIM

 
 
* 607074

PROTEIN KINASE D2; PRKD2


Alternative titles; symbols

PKD2


HGNC Approved Gene Symbol: PRKD2

Cytogenetic location: 19q13.32     Genomic coordinates (GRCh38): 19:46,674,316-46,717,114 (from NCBI)


TEXT

▼ Cloning and Expression

By EST database searching with the conserved C-terminal sequence of serine kinases and screening of a human ductal pancreatic cDNA library, Sturany et al. (2001) cloned a novel serine/threonine protein kinase, which they called PKD2. The deduced 878-amino acid protein, which has a predicted molecular mass of 97 kD, contains an N-terminal hydrophobic area predicted to be a transmembrane region, 2 cysteine-rich motifs that form zinc finger-like repeats, a pleckstrin homology domain, and a putative kinase domain containing the ATP-binding consensus sequence. An invariant aspartate essential for kinase activity is located within a motif conserved in serine/threonine kinases. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu (PRKCM; 605435) and PKC-nu (PRKCN; 607077). Northern blot analysis detected constitutive low expression of a 4-kb transcript; higher levels were observed in pancreas, heart, lung, smooth muscle, and brain, and lower levels kidney and liver. SDS-PAGE revealed a protein with a molecular mass of 105 kD, as did Western blot analysis of endogenous PRKD2 or protein expressed following transfection.


▼ Gene Function

Sturany et al. (2001) demonstrated phorbol ester binding to the duplex zinc finger-like motif of PRKD2 as well as autophosphorylation following phorbol ester stimulation. The addition of phosphatidyl-L-serine caused synergistic stimulation. Phorbol ester-activated PRKD2 migrated more slowly than inactive PRKD2 by SDS-PAGE, suggesting hyperphosphorylation upon activation.


▼ Mapping

The International Radiation Hybrid Mapping Consortium mapped the PRKD2 gene to chromosome 19 (R67403).

▼ REFERENCES

  1. Sturany, S., Van Lint, J., Muller, F., Wilda, M., Hameister, H., Hocker, M., Brey, A., Gern, U., Vandenheede, J., Gress, T., Adler, G., Seufferlein, T. Molecular cloning and characterization of the human protein kinase D2: a novel member of the protein kinase D family of serine threonine kinases. J. Biol. Chem. 276: 3310-3318, 2001. [PubMed: 11062248, related citations] [Full Text]


Creation Date:
Patricia A. Hartz : 6/26/2002
Edit History:
alopez : 04/20/2011
carol : 6/27/2002
carol : 6/27/2002

* 607074

PROTEIN KINASE D2; PRKD2


Alternative titles; symbols

PKD2


HGNC Approved Gene Symbol: PRKD2

Cytogenetic location: 19q13.32     Genomic coordinates (GRCh38): 19:46,674,316-46,717,114 (from NCBI)


TEXT

Cloning and Expression

By EST database searching with the conserved C-terminal sequence of serine kinases and screening of a human ductal pancreatic cDNA library, Sturany et al. (2001) cloned a novel serine/threonine protein kinase, which they called PKD2. The deduced 878-amino acid protein, which has a predicted molecular mass of 97 kD, contains an N-terminal hydrophobic area predicted to be a transmembrane region, 2 cysteine-rich motifs that form zinc finger-like repeats, a pleckstrin homology domain, and a putative kinase domain containing the ATP-binding consensus sequence. An invariant aspartate essential for kinase activity is located within a motif conserved in serine/threonine kinases. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu (PRKCM; 605435) and PKC-nu (PRKCN; 607077). Northern blot analysis detected constitutive low expression of a 4-kb transcript; higher levels were observed in pancreas, heart, lung, smooth muscle, and brain, and lower levels kidney and liver. SDS-PAGE revealed a protein with a molecular mass of 105 kD, as did Western blot analysis of endogenous PRKD2 or protein expressed following transfection.


Gene Function

Sturany et al. (2001) demonstrated phorbol ester binding to the duplex zinc finger-like motif of PRKD2 as well as autophosphorylation following phorbol ester stimulation. The addition of phosphatidyl-L-serine caused synergistic stimulation. Phorbol ester-activated PRKD2 migrated more slowly than inactive PRKD2 by SDS-PAGE, suggesting hyperphosphorylation upon activation.


Mapping

The International Radiation Hybrid Mapping Consortium mapped the PRKD2 gene to chromosome 19 (R67403).

REFERENCES

  1. Sturany, S., Van Lint, J., Muller, F., Wilda, M., Hameister, H., Hocker, M., Brey, A., Gern, U., Vandenheede, J., Gress, T., Adler, G., Seufferlein, T. Molecular cloning and characterization of the human protein kinase D2: a novel member of the protein kinase D family of serine threonine kinases. J. Biol. Chem. 276: 3310-3318, 2001. [PubMed: 11062248] [Full Text: https://doi.org/10.1074/jbc.M008719200]


Creation Date:
Patricia A. Hartz : 6/26/2002

Edit History:
alopez : 04/20/2011
carol : 6/27/2002
carol : 6/27/2002



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OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.

NOTE: OMIM is intended for use primarily by physicians and other professionals concerned with genetic disorders, by genetics researchers, and by advanced students in science and medicine. While the OMIM database is open to the public, users seeking information about a personal medical or genetic condition are urged to consult with a qualified physician for diagnosis and for answers to personal questions.
OMIM® and Online Mendelian Inheritance in Man® are registered trademarks of the Johns Hopkins University.
Copyright® 1966-2024 Johns Hopkins University.
Printed: April 24, 2024