HGNC Approved Gene Symbol: SCAMP2
Cytogenetic location: 15q24.1 Genomic coordinates (GRCh38): 15:74,843,730-74,873,365 (from NCBI)
Singleton et al. (1997) amplified SCAMP2 by PCR using primers designed from SCAMP1 (606911). They cloned the full-length SCAMP2 cDNA from a HeLa cell cDNA library. They also identified SCAMP3 (606913) as another paralog in the HeLa cell library. SCAMP2 encodes a deduced 329-amino acid protein with a calculated molecular mass of 36.6 kD. The protein shares structural features with SCAMP1 and SCAMP3, including a leucine zipper-like segment, a proline-rich element, an extended central core that includes 4 putative transmembrane domains, a polar segment, and an alanine-rich C terminus. SCAMP2 shares 54% and 56% overall sequence identity with SCAMP1 and SCAMP3, respectively, and 94% identity with the mouse homolog. The most divergent regions are in the N terminus. Northern blot analysis detected a 1.3-kb SCAMP2 transcript, with highest expression in heart, placenta, and skeletal muscle, intermediate expression in lung, pancreas, and liver, and low expression in brain and kidney. Immunofluorescent localization in HeLa cells showed punctate staining with some perinuclear and peripheral enrichment, and partial colocalization with SCAMP1 and SCAMP3.
The International Radiation Hybrid Mapping Consortium mapped the SCAMP2 gene to chromosome 15 (sts-W74766).
Singleton, D. R., Wu, T. T., Castle, J. D. Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions. J. Cell Sci. 110: 2099-2107, 1997. [PubMed: 9378760] [Full Text: https://doi.org/10.1242/jcs.110.17.2099]