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Cell senescence : methods and protocols

Author(s):
Galluzzi, Lorenzo
Title(s):
Cell senescence : methods and protocols / edited by Lorenzo Galluzzi ... [et al.].
Series:
Methods in molecular biology, ; 965 ISSN 1064-3745
Springer protocols
Country of Publication:
United States
Publisher:
New York : Humana Press ; Springer, c2013.
Description:
xii, 538 p. : ill. (some col.) ; 26 cm.
Language:
English
ISBN:
9781627032391 (eBook)
1627032398 (eBook)
9781627032384 (alk. paper)
162703238X (alk. paper)
LCCN:
2012950333
Electronic Links:
http://www.springerprotocols.com/BookToc/doi/10.1007/978-1-62703-239-1
MeSH:
Cellular Senescence*
Cytological Techniques/methods
Publication Type(s):
Laboratory Manual
Notes:
Includes bibliographical references and index.
Also issued online.
Contents:
Cell senescence as both a dynamic and a static phenotype / Andrew R.J. Young, Masako Narita, and Masashi Narita -- Senescence regulation by mTOR / Vjekoslav Dulic -- Senescence regulation by the p53 protein family / Yingjuan Qian and Xinbin Chen --- Markers of cellular senescence / Amancio Carnero -- Biomarkers of cell senescence assessed by imaging cytometry / Hong Zhao and Zbigniew Darzynkiewicz -- Cytofluorometric assessment of cell cycle progression / Ilio Vitale ... [et al.] -- Quantification of cell cycle-arresting proteins / Oliver Kepp ... [et al.] -- Colorimetric Detection of Senescence-Associated € galactosidase / Koji Itahana, Yoko Itahana, and Goberdhan P. Dimri -- Chemiluminescent detection of senescence-associated € galactosidase / Vinicius Bassaneze, Ayumi Aurea Miyakawa, and José Eduardo Krieger -- Detection of the senescence-associated secretory phenotype (SASP) / Francis Rodier -- Unbiased characterization of the senescence-associated secretome using SILAC-based quantitative proteomics / Juan Carlos Acosta, Ambrosius P. Snijders, and Jesús Gil -- Detection of senescence-associated heterochromatin foci (SAHF) / Katherine M. Aird and Rugang Zhang -- Monitoring DNA damage during cell senescence / Glyn Nelson and Thomas von Zglinicki -- Assessment and quantification of telomerase enzyme activity / Michelle F. Maritz, Laura A. Richards, and Karen L. MacKenzie -- Methods for the assessment of telomere status / Asako J. Nakamura -- Detection of nuclear envelope alterations in senescence / Clea Bárcena, Fernando G. Osorio, and José Maria Pérez Freije -- Measuring reactive oxygen species in senescent cells / João F. Passos, Satomi Miwa, and Thomas von Zglinicki -- Quantification of protein carbonylation / Nancy B. Wehr and Rodney L. Levine -- Assays for the measurement of lipid peroxidation / Ana Cipak Gasparovic ... [et al.] -- Raman spectroscopy for the detection of AGEs/ALEs / J. Renwick Beattie, John J. McGarvey, and Alan W. Stitt -- Monitoring oncogenic B-RAF-induced senescence in melanocytes / Sieu L. Tran and Helen Rizos -- Methods to investigate the role of SIRT1 in endothelial senescence / Bo Bai and Yu Wang -- Monitoring nutrient signaling through the longevity protein p66SHC1 / Sofia Chiatamone Ranieri and Giovambattista Pani -- Profiling the metabolic signature of senescence / Florian M. Geier ... [et al.] -- Genome-wide RNAi screening to identify regulators of oncogene-induced cellular senescence / Narendra Wajapeyee, Sara K. Deibler, and Michael R. Green -- Integrated approach for monitoring cell senescence / Tatiana V. Pospelova, Zhanna V. Chitikova, and Valery A. Pospelov -- Robust multiparametric assessment of cellular senescence / Clara Correia-Melo, Diana Jurk, and João F. Passos -- Assessing chronological aging in bacteria / Stavros Gonidakis and Valter D. Longo -- Assessing organismal aging in the filamentous fungus Podospora anserina / Heinz D. Osiewacz, Andrea Hamann, and Sandra Zintel -- Assessing chronological aging in Saccharomyces cerevisiae / Jia Hu ... [et al.] -- Assessing aging and senescent decline in Caenorhabditis elegans : cohort survival analysis / Eirini Lionaki and Nektarios Tavernarakis -- High-throughput and longitudinal analysis of aging and senescent decline in Caenorhabditis elegans / Eirini Lionaki and Nektarios Tavernarakis -- Assessing senescence in Drosophila using video tracking / Reza Ardekani, Simon Tavaré, and John Tower -- Assessing vascular senescence in zebrafish / Sandra Donnini, Antonio Giachetti, and Marina Ziche.
NLM ID:
101601894 [Book]

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