My Bibliography

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Youn T, Ehsan M, Hariharan P, Li X, Moon Y, Ahmed W, Byrne B, Liu X, Guan L, Chae PS. Tailoring butane-1,2,3,4-tetraol-based maltosides (BTMs) via group-swapping and detergent unsymmetry: new detergent design strategies for membrane protein studies. J Mater Chem B. 2025 Sep 8;. doi: 10.1039/d5tb01342a. [Epub ahead of print] PubMed PMID: 40916887; PubMed Central PMCID: PMC12417679.

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Youn T, Ehsan M, Hariharan P, Li X, Moon Y, Ahmed W, Byrne B, Liu X, Guan L, Chaea PS. ailoring butane-1,2,3,4-tetraol-based maltosides (BTMs) via group-swapping and unsymmetry: New detergent design strategies for membrane protein studies. Journal of Materials Chemistry B. 2025 August.

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Youn T, Kim G, Hariharan P, Li X, Ahmed W, Byrne B, Liu X, Guan L, Chae PS. Improved Pendant-Bearing Glucose-Neopentyl Glycols for Membrane Protein Stability. Bioconjug Chem. 2025 Apr 16;36(4):707-717. doi: 10.1021/acs.bioconjchem.4c00556. Epub 2025 Mar 19. PubMed PMID: 40105011; PubMed Central PMCID: PMC12110327.

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Sadaf A, Yun HS, Lee H, Stanfield S, Lan B, Salomon K, Woubshete M, Kim S, Ehsan M, Bae H, Byrne B, Loland CJ, Liu X, Guan L, Im W, Chae PS. Multiple Pendants-Bearing Triglucosides for Membrane Protein Studies: Effects of Pendant Length and Number on Micelle Interior Hydration and Protein Stability. Biomacromolecules. 2025 Apr 14;26(4):2565-2579. doi: 10.1021/acs.biomac.5c00036. Epub 2025 Mar 14. PubMed PMID: 40087026; PubMed Central PMCID: PMC12165317.

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Ehsan M, Ghani L, Lan B, Katsube S, Poulsen IH, Zhang X, Arslan M, Byrne B, Loland CJ, Guan L, Liu X, Chae PS. Unsymmetric Triazine-Based Triglucoside Detergents for Membrane Protein Stability. Chembiochem. 2025 Feb 3;26(5):e202400958. doi: 10.1002/cbic.202400958. Epub 2025 Jan 28. PubMed PMID: 39779472; PubMed Central PMCID: PMC11875885.

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Liang R, Guan L. Atomic-Level Free Energy Landscape Reveals Cooperative Symport Mechanism of Melibiose Transporter. bioRxiv. 2024 Sep 14;. doi: 10.1101/2024.08.21.608993. PubMed PMID: 39229106; PubMed Central PMCID: PMC11370570.

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Hariharan P, Guan L. Reconstitution of the Melibiose Permease of Salmonella enterica serovar Typhimurium (MelB(St)) into Lipid Nanodiscs. Bio Protoc. 2024 Aug 5;14(15):e5045. doi: 10.21769/BioProtoc.5045. eCollection 2024 Aug 5. PubMed PMID: 39131193; PubMed Central PMCID: PMC11309958.

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Hariharan P, Bakhtiiari A, Liang R, Guan L. Distinct roles of the major binding residues in the cation-binding pocket of the melibiose transporter MelB. J Biol Chem. 2024 Jul;300(7):107427. doi: 10.1016/j.jbc.2024.107427. Epub 2024 May 31. PubMed PMID: 38823641; PubMed Central PMCID: PMC11259710.

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Hariharan P, Bakhtiiari A, Liang R, Guan L. Distinct roles of the major binding residues in the cation-binding pocket of MelB. bioRxiv. 2024 Mar 2;. doi: 10.1101/2024.02.27.582382. PubMed PMID: 38464317; PubMed Central PMCID: PMC10925273.

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Hariharan P, Shi Y, Katsube S, Willibal K, Burrows ND, Mitchell P, Bakhtiiari A, Stanfield S, Pardon E, Kaback HR, Liang R, Steyaert J, Viner R, Guan L. Mobile barrier mechanisms for Na(+)-coupled symport in an MFS sugar transporter. Elife. 2024 Feb 21;12. doi: 10.7554/eLife.92462. PubMed PMID: 38381130; PubMed Central PMCID: PMC10942615.

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Yoon S, Bae HE, Hariharan P, Nygaard A, Lan B, Woubshete M, Sadaf A, Liu X, Loland CJ, Byrne B, Guan L, Chae PS. Rational Approach to Improve Detergent Efficacy for Membrane Protein Stabilization. Bioconjug Chem. 2024 Feb 21;35(2):223-231. doi: 10.1021/acs.bioconjchem.3c00507. Epub 2024 Jan 12. PubMed PMID: 38215010; PubMed Central PMCID: PMC10970486.

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Guan L. The rapid developments of membrane protein structure biology over the last two decades. BMC Biol. 2023 Dec 29;21(1):300. doi: 10.1186/s12915-023-01795-9. PubMed PMID: 38155357; PubMed Central PMCID: PMC10755942.

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Hariharan P, Shi Y, Katsube S, Willibal K, Burrows ND, Mitchell P, Bakhtiiari A, Stanfield S, Pardon E, Kaback HR, Liang R, Steyaert J, Viner R, Guan L. Mobile barrier mechanisms for Na(+)-coupled symport in an MFS sugar transporter. bioRxiv. 2023 Nov 25;. doi: 10.1101/2023.09.18.558283. PubMed PMID: 37790566; PubMed Central PMCID: PMC10542114.

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Ghani L, Kim S, Ehsan M, Lan B, Poulsen IH, Dev C, Katsube S, Byrne B, Guan L, Loland CJ, Liu X, Im W, Chae PS. Melamine-cored glucosides for membrane protein solubilization and stabilization: importance of water-mediated intermolecular hydrogen bonding in detergent performance. Chem Sci. 2023 Nov 22;14(45):13014-13024. doi: 10.1039/d3sc03543c. eCollection 2023 Nov 22. PubMed PMID: 38023530; PubMed Central PMCID: PMC10664503.

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Blaimschein N, Parameswaran H, Nagler G, Manioglu S, Helenius J, Ardelean C, Kuhn A, Guan L, Müller DJ. The insertase YidC chaperones the polytopic membrane protein MelB inserting and folding simultaneously from both termini. Structure. 2023 Nov 2;31(11):1419-1430.e5. doi: 10.1016/j.str.2023.08.012. Epub 2023 Sep 13. PubMed PMID: 37708891; PubMed Central PMCID: PMC10840855.

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Katsube S, Willibal K, Vemulapally S, Hariharan P, Tikhonova E, Pardon E, Kaback HR, Steyaert J, Guan L. In vivo and in vitro characterizations of melibiose permease (MelB) conformation-dependent nanobodies reveal sugar-binding mechanisms. J Biol Chem. 2023 Aug;299(8):104967. doi: 10.1016/j.jbc.2023.104967. Epub 2023 Jun 26. PubMed PMID: 37380079; PubMed Central PMCID: PMC10374971.

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Panda A, Giska F, Duncan AL, Welch AJ, Brown C, McAllister R, Hariharan P, Goder JND, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman JE, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nat Methods. 2023 Jun;20(6):891-897. doi: 10.1038/s41592-023-01864-5. Epub 2023 Apr 27. PubMed PMID: 37106230; PubMed Central PMCID: PMC10932606.

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Ghani L, Zhang X, Munk CF, Hariharan P, Lan B, Yun HS, Byrne B, Guan L, Loland CJ, Liu X, Chae PS. Tris(hydroxymethyl)aminomethane Linker-Bearing Triazine-Based Triglucosides for Solubilization and Stabilization of Membrane Proteins. Bioconjug Chem. 2023 Apr 19;34(4):739-747. doi: 10.1021/acs.bioconjchem.3c00042. Epub 2023 Mar 15. PubMed PMID: 36919927; PubMed Central PMCID: PMC10145683.

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Chua GL, Tan BC, Loke RYJ, He M, Chin CF, Wong BH, Kuk ACY, Ding M, Wenk MR, Guan L, Torta F, Silver DL. Mfsd2a utilizes a flippase mechanism to mediate omega-3 fatty acid lysolipid transport. Proc Natl Acad Sci U S A. 2023 Mar 7;120(10):e2215290120. doi: 10.1073/pnas.2215290120. Epub 2023 Feb 27. PubMed PMID: 36848557; PubMed Central PMCID: PMC10013850.

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Blaimschein N, Hariharan P, Manioglu S, Guan L, Müller DJ. Substrate-binding guides individual melibiose permeases MelB to structurally soften and to destabilize cytoplasmic middle-loop C3. Structure. 2023 Jan 5;31(1):58-67.e4. doi: 10.1016/j.str.2022.11.011. Epub 2022 Dec 15. PubMed PMID: 36525976; PubMed Central PMCID: PMC9825662.

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Lee HS, Das M, Mahler F, Ahmed W, Wang H, Mortensen JS, Hariharan P, Ghani L, Byrne B, Guan L, Loland CJ, Keller S, Chae PS. 3,4-Bis(hydroxymethyl)hexane-1,6-diol-based Maltosides (HDMs) for Membrane-Protein Study: Importance of Detergent Rigidity-Flexibility Balance in Protein Stability. Chem Asian J. 2022 Dec 14;17(24):e202200941. doi: 10.1002/asia.202200941. Epub 2022 Nov 8. PubMed PMID: 36253323.

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Guan L. Structure and mechanism of membrane transporters. Sci Rep. 2022 Aug 2;12(1):13248. doi: 10.1038/s41598-022-17524-1. PubMed PMID: 35918457; PubMed Central PMCID: PMC9345868.

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Katsube S, Liang R, Amin A, Hariharan P, Guan L. Molecular Basis for the Cation Selectivity of Salmonella typhimurium Melibiose Permease. J Mol Biol. 2022 Jun 30;434(12):167598. doi: 10.1016/j.jmb.2022.167598. Epub 2022 Apr 22. PubMed PMID: 35461877; PubMed Central PMCID: PMC9177823.

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Lee HJ, Ehsan M, Zhang X, Katsube S, Munk CF, Wang H, Ahmed W, Kumar A, Byrne B, Loland CJ, Guan L, Liu X, Chae PS. Development of 1,3-acetonedicarboxylate-derived glucoside amphiphiles (ACAs) for membrane protein study. Chem Sci. 2022 May 18;13(19):5750-5759. doi: 10.1039/d2sc00539e. eCollection 2022 May 18. PubMed PMID: 35694361; PubMed Central PMCID: PMC9116450.

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Ghani L, Kim S, Wang H, Lee HS, Mortensen JS, Katsube S, Du Y, Sadaf A, Ahmed W, Byrne B, Guan L, Loland CJ, Kobilka BK, Im W, Chae PS. Foldable Detergents for Membrane Protein Study: Importance of Detergent Core Flexibility in Protein Stabilization. Chemistry. 2022 Apr 12;28(21):e202200116. doi: 10.1002/chem.202200116. Epub 2022 Mar 15. PubMed PMID: 35238091; PubMed Central PMCID: PMC9007890.

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Ehsan M, Wang H, Katsube S, Munk CF, Du Y, Youn T, Yoon S, Byrne B, Loland CJ, Guan L, Kobilka BK, Chae PS. Glyco-Steroidal Amphiphiles (GSAs) for Membrane Protein Structural Study. Chembiochem. 2022 Apr 5;23(7):e202200027. doi: 10.1002/cbic.202200027. Epub 2022 Feb 21. PubMed PMID: 35129249; PubMed Central PMCID: PMC8986615.

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Ehsan M, Wang H, Cecchetti C, Mortensen JS, Du Y, Hariharan P, Nygaard A, Lee HJ, Ghani L, Guan L, Loland CJ, Byrne B, Kobilka BK, Chae PS. Maltose-bis(hydroxymethyl)phenol (MBPs) and Maltose-tris(hydroxymethyl)phenol (MTPs) Amphiphiles for Membrane Protein Stability. ACS Chem Biol. 2021 Sep 17;16(9):1779-1790. doi: 10.1021/acschembio.1c00578. Epub 2021 Aug 26. PubMed PMID: 34445864; PubMed Central PMCID: PMC8482870.

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Markham KJ, Tikhonova EB, Scarpa AC, Hariharan P, Katsube S, Guan L. Complete cysteine-scanning mutagenesis of the Salmonella typhimurium melibiose permease. J Biol Chem. 2021 Sep;297(3):101090. doi: 10.1016/j.jbc.2021.101090. Epub 2021 Aug 18. PubMed PMID: 34416232; PubMed Central PMCID: PMC8437787.

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Guan L, Hariharan P. X-ray crystallography reveals molecular recognition mechanism for sugar binding in a melibiose transporter MelB. Commun Biol. 2021 Aug 2;4(1):931. doi: 10.1038/s42003-021-02462-x. PubMed PMID: 34341464; PubMed Central PMCID: PMC8329300.

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Hariharan P, Guan L. Cooperative binding ensures the obligatory melibiose/Na+ cotransport in MelB. J Gen Physiol. 2021 Aug 2;153(8). doi: 10.1085/jgp.202012710. Epub 2021 Jun 10. PubMed PMID: 34110360; PubMed Central PMCID: PMC8200842.

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Sadaf A, Kim S, Bae HE, Wang H, Nygaard A, Uegaki Y, Du Y, Munk CF, Katsube S, Sung Lee H, Bae J, Choi CW, Choi HJ, Byrne B, Gellman SH, Guan L, Loland CJ, Kobilka BK, Im W, Chae PS. Conformationally flexible core-bearing detergents with a hydrophobic or hydrophilic pendant: Effect of pendant polarity on detergent conformation and membrane protein stability. Acta Biomater. 2021 Jul 1;128:393-407. doi: 10.1016/j.actbio.2021.04.043. Epub 2021 Apr 29. PubMed PMID: 33933694; PubMed Central PMCID: PMC8222176.

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Guan L. Molecular recognition of sugar binding in a melibiose transporter MelB by X-ray crystallography. bioRxiv : the preprint server for biology. 2020 December.

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Das M, Mahler F, Hariharan P, Wang H, Du Y, Mortensen JS, Patallo EP, Ghani L, Glück D, Lee HJ, Byrne B, Loland CJ, Guan L, Kobilka BK, Keller S, Chae PS. Diastereomeric Cyclopentane-Based Maltosides (CPMs) as Tools for Membrane Protein Study. J Am Chem Soc. 2020 Dec 23;142(51):21382-21392. doi: 10.1021/jacs.0c09629. Epub 2020 Dec 14. PubMed PMID: 33315387; PubMed Central PMCID: PMC8015409.

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Guan L. Glucose/Sugar TransportinBacteria. Preprint the Life Sciences Reference Collection. 2020 November.

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Bae HE, Cecchetti C, Du Y, Katsube S, Mortensen JS, Huang W, Rehan S, Lee HJ, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PS. Pendant-bearing glucose-neopentyl glycol (P-GNG) amphiphiles for membrane protein manipulation: Importance of detergent pendant chain for protein stabilization. Acta Biomater. 2020 Aug;112:250-261. doi: 10.1016/j.actbio.2020.06.001. Epub 2020 Jun 6. PubMed PMID: 32522715; PubMed Central PMCID: PMC7366829.

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Ehsan M, Katsube S, Cecchetti C, Du Y, Mortensen JS, Wang H, Nygaard A, Ghani L, Loland CJ, Kobilka BK, Byrne B, Guan L, Chae PS. New Malonate-Derived Tetraglucoside Detergents for Membrane Protein Stability. ACS Chem Biol. 2020 Jun 19;15(6):1697-1707. doi: 10.1021/acschembio.0c00316. Epub 2020 Jun 5. PubMed PMID: 32501004; PubMed Central PMCID: PMC7410094.

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Ghani L, Munk CF, Zhang X, Katsube S, Du Y, Cecchetti C, Huang W, Bae HE, Saouros S, Ehsan M, Guan L, Liu X, Loland CJ, Kobilka BK, Byrne B, Chae PS. 1,3,5-Triazine-Cored Maltoside Amphiphiles for Membrane Protein Extraction and Stabilization. J Am Chem Soc. 2019 Dec 18;141(50):19677-19687. doi: 10.1021/jacs.9b07883. Epub 2019 Dec 6. PubMed PMID: 31809039; PubMed Central PMCID: PMC7220817.

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Tilegenova C, Cortes DM, Jahovic N, Hardy E, Hariharan P, Guan L, Cuello LG. Structure, function, and ion-binding properties of a K(+) channel stabilized in the 2,4-ion-bound configuration. Proc Natl Acad Sci U S A. 2019 Aug 20;116(34):16829-16834. doi: 10.1073/pnas.1901888116. Epub 2019 Aug 6. PubMed PMID: 31387976; PubMed Central PMCID: PMC6708363.

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Ehsan M, Du Y, Mortensen JS, Hariharan P, Qu Q, Ghani L, Das M, Grethen A, Byrne B, Skiniotis G, Keller S, Loland CJ, Guan L, Kobilka BK, Chae PS. Self-Assembly Behavior and Application of Terphenyl-Cored Trimaltosides for Membrane-Protein Studies: Impact of Detergent Hydrophobic Group Geometry on Protein Stability. Chemistry. 2019 Sep 2;25(49):11545-11554. doi: 10.1002/chem.201902468. Epub 2019 Aug 5. PubMed PMID: 31243822; PubMed Central PMCID: PMC7239454.

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Sadaf A, Ramos M, Mortensen JS, Du Y, Bae HE, Munk CF, Hariharan P, Byrne B, Kobilka BK, Loland CJ, Guan L, Chae PS. Conformationally Restricted Monosaccharide-Cored Glycoside Amphiphiles: The Effect of Detergent Headgroup Variation on Membrane Protein Stability. ACS Chem Biol. 2019 Aug 16;14(8):1717-1726. doi: 10.1021/acschembio.9b00166. Epub 2019 Jul 29. PubMed PMID: 31305987; PubMed Central PMCID: PMC7233146.

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Kaback HR, Guan L. It takes two to tango: The dance of the permease. J Gen Physiol. 2019 Jul 1;151(7):878-886. doi: 10.1085/jgp.201912377. Epub 2019 May 30. Review. PubMed PMID: 31147449; PubMed Central PMCID: PMC6605686.

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Ehsan M, Kumar A, Mortensen JS, Du Y, Hariharan P, Kumar KK, Ha B, Byrne B, Guan L, Kobilka BK, Loland CJ, Chae PS. Self-Assembly Behaviors of a Penta-Phenylene Maltoside and Its Application for Membrane Protein Study. Chem Asian J. 2019 Jun 3;14(11):1926-1931. doi: 10.1002/asia.201900224. Epub 2019 Apr 10. PubMed PMID: 30969484; PubMed Central PMCID: PMC7239035.

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Das M, Du Y, Mortensen JS, Ramos M, Ghani L, Lee HJ, Bae HE, Byrne B, Guan L, Loland CJ, Kobilka BK, Chae PS. Trehalose-cored amphiphiles for membrane protein stabilization: importance of the detergent micelle size in GPCR stability. Org Biomol Chem. 2019 Mar 20;17(12):3249-3257. doi: 10.1039/c8ob03153c. PubMed PMID: 30843907.

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Guan L, Kaback H. Lactose Permease: From Membrane to Molecule to Mechanism. In: Handbook of Hydrocarbon and Lipid Microbiology, sss: Springer International Publishing; 2019.

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Hussain H, Helton T, Du Y, Mortensen JS, Hariharan P, Ehsan M, Byrne B, Loland CJ, Kobilka BK, Guan L, Chae PS. A comparative study of branched and linear mannitol-based amphiphiles on membrane protein stability. Analyst. 2018 Nov 19;143(23):5702-5710. doi: 10.1039/c8an01408f. PubMed PMID: 30334564; PubMed Central PMCID: PMC6242751.

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Bae HE, Du Y, Hariharan P, Mortensen JS, Kumar KK, Ha B, Das M, Lee HS, Loland CJ, Guan L, Kobilka BK, Chae PS. Asymmetric maltose neopentyl glycol amphiphiles for a membrane protein study: effect of detergent asymmetricity on protein stability. Chem Sci. 2019 Jan 28;10(4):1107-1116. doi: 10.1039/c8sc02560f. eCollection 2019 Jan 28. PubMed PMID: 30774908; PubMed Central PMCID: PMC6346398.

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Das M, Du Y, Mortensen JS, Hariharan P, Lee HS, Byrne B, Loland CJ, Guan L, Kobilka BK, Chae PS. Rationally Engineered Tandem Facial Amphiphiles for Improved Membrane Protein Stabilization Efficacy. Chembiochem. 2018 Oct 18;19(20):2225-2232. doi: 10.1002/cbic.201800388. Epub 2018 Sep 21. PubMed PMID: 30070754; PubMed Central PMCID: PMC7233144.

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Hariharan P, Tikhonova E, Medeiros-Silva J, Jeucken A, Bogdanov MV, Dowhan W, Brouwers JF, Weingarth M, Guan L. Structural and functional characterization of protein-lipid interactions of the Salmonella typhimurium melibiose transporter MelB. BMC Biol. 2018 Aug 3;16(1):85. doi: 10.1186/s12915-018-0553-0. PubMed PMID: 30075778; PubMed Central PMCID: PMC6091025.

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Ehsan M, Das M, Stern V, Du Y, Mortensen JS, Hariharan P, Byrne B, Loland CJ, Kobilka BK, Guan L, Chae PS. Steroid-Based Amphiphiles for Membrane Protein Study: The Importance of Alkyl Spacers for Protein Stability. Chembiochem. 2018 Jul 4;19(13):1433-1443. doi: 10.1002/cbic.201800106. Epub 2018 May 29. PubMed PMID: 29660780; PubMed Central PMCID: PMC7238963.

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Ehsan M, Du Y, Molist I, Seven AB, Hariharan P, Mortensen JS, Ghani L, Loland CJ, Skiniotis G, Guan L, Byrne B, Kobilka BK, Chae PS. Vitamin E-based glycoside amphiphiles for membrane protein structural studies. Org Biomol Chem. 2018 Apr 4;16(14):2489-2498. doi: 10.1039/C8OB00270C. PubMed PMID: 29564464.