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Flow Cytometry

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

Year introduced: 1982

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Subheadings:

Tree Number(s): E01.370.225.500.363.342, E01.370.225.500.386.350, E05.196.712.516.600.240.350, E05.200.500.363.342, E05.200.500.386.350, E05.242.363.342, E05.242.386.350

MeSH Unique ID: D005434

Entry Terms:

  • Microfluorometry, Flow
  • Flow Microfluorometries
  • Flow Microfluorometry
  • Microfluorometries, Flow
  • Flow Microfluorimetry
  • Microfluorimetry, Flow
  • Cytofluorometry, Flow
  • Cytofluorometries, Flow
  • Flow Cytofluorometries
  • Flow Cytofluorometry
  • Cytometry, Flow
  • Cytometries, Flow
  • Flow Cytometries
  • Fluorescence-Activated Cell Sorting
  • Cell Sorting, Fluorescence-Activated
  • Cell Sortings, Fluorescence-Activated
  • Fluorescence Activated Cell Sorting
  • Fluorescence-Activated Cell Sortings
  • Sorting, Fluorescence-Activated Cell
  • Sortings, Fluorescence-Activated Cell

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