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Bioconversion of Stevioside to Rebaudioside E Using Glycosyltransferase UGTSL2.
Chen L, Pan H, Cai R, Li Y, Jia H, Chen K, Yan M, Ouyang P. Chen L, et al. Appl Biochem Biotechnol. 2021 Mar;193(3):637-649. doi: 10.1007/s12010-020-03439-y. Epub 2020 Oct 15. Appl Biochem Biotechnol. 2021. PMID: 33057971
Rebaudioside E, one of the minor components of steviol glycosides, was first isolated and identified from Stevia rebaudiana in 1977. ...In a 20-ml bioconversion reaction of 20 g/l stevioside by UGTSL2 and StSUS1, 15.92 g/l rebaudioside E was produced f
Rebaudioside E, one of the minor components of steviol glycosides, was first isolated and identified from Stevia rebaudiana in
Identification of sucrose synthase from Micractinium conductrix to favor biocatalytic glycosylation.
Chen K, Lin L, Ma R, Ding J, Pan H, Tao Y, Li Y, Jia H. Chen K, et al. Front Microbiol. 2023 Aug 15;14:1220208. doi: 10.3389/fmicb.2023.1220208. eCollection 2023. Front Microbiol. 2023. PMID: 37649634 Free PMC article.
When the mutant S31D of McSuSy was applied by coupling the engineered Stevia glycosyltransferase UGT76G1 in a one-pot two-enzyme reaction at 10% DMSO, 50 g/L rebaudioside E was transformed into 51.06 g/L rebaudioside M in 57 h by means of batch feeding, with a yield …
When the mutant S31D of McSuSy was applied by coupling the engineered Stevia glycosyltransferase UGT76G1 in a one-pot two-enzyme reaction at …
Identification of the Key Residues of the Uridine Diphosphate Glycosyltransferase 91D2 and its Effect on the Accumulation of Steviol Glycosides in Stevia rebaudiana.
Zhang S, Yang Y, Lyu C, Chen J, Li D, Liu Y, Zhang Z, Liu Y, Wu W. Zhang S, et al. J Agric Food Chem. 2021 Feb 17;69(6):1852-1863. doi: 10.1021/acs.jafc.0c07066. Epub 2021 Feb 7. J Agric Food Chem. 2021. PMID: 33550805
In the metabolic glycosylation grid of SGs, SrUGT91D2 has been shown to catalyze formation of 1,2-beta-d-glucoside linkages at the C(13)- and C(19)-positions and play a crucial role in the synthesis of SGs, including the formation of stevioside (ST), rebaudioside E
In the metabolic glycosylation grid of SGs, SrUGT91D2 has been shown to catalyze formation of 1,2-beta-d-glucoside linkages at the C(13)- an …
Minor diterpene glycosides from the leaves of Stevia rebaudiana.
Ibrahim MA, Rodenburg DL, Alves K, Fronczek FR, McChesney JD, Wu C, Nettles BJ, Venkataraman SK, Jaksch F. Ibrahim MA, et al. J Nat Prod. 2014 May 23;77(5):1231-5. doi: 10.1021/np4009656. Epub 2014 Apr 23. J Nat Prod. 2014. PMID: 24758242
Compound 1 (rebaudioside KA) was shown to be 13-[(O-beta-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid 2-O-beta-d-glucopyranosyl-beta-d-glucopyranosyl ester and compound 2, 12-alpha-[(2-O-beta-d-glucopyranosyl-beta-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid beta-d-glucopyr …
Compound 1 (rebaudioside KA) was shown to be 13-[(O-beta-d-glucopyranosyl)oxy]ent-kaur-16-en-19-oic acid 2-O-beta-d-glucopyranosyl-beta-d-gl …