Diverse and Flexible Transmission of fosA3 Associated with Heterogeneous Multidrug Resistance Regions in Salmonella enterica Serovar Typhimurium and Indiana Isolates

Liang-Xing Fang; Qi Jiang; Guo-Hui Deng; Bing He; Ruan-Yang Sun; Jin-Fei Zhang; Dao-Ji Cen; Yuan-Yuan Miao; Dong Wang; Wen-Ying Guo; Jian Sun; Ya-Hong Liu; Xiao-Ping Liao

doi:10.1128/AAC.02001-19

Diverse and Flexible Transmission of fosA3 Associated with Heterogeneous Multidrug Resistance Regions in Salmonella enterica Serovar Typhimurium and Indiana Isolates

Antimicrob Agents Chemother. 2020 Jan 27;64(2):e02001-19. doi: 10.1128/AAC.02001-19. Print 2020 Jan 27.

Authors

Liang-Xing Fang^{1

2

3}, Qi Jiang^{1

2

3}, Guo-Hui Deng^{1

2

3}, Bing He^{1

2

3}, Ruan-Yang Sun^{1

2

3}, Jin-Fei Zhang^{1

2

3}, Dao-Ji Cen^{1

2

3}, Yuan-Yuan Miao^{1

2

3}, Dong Wang^{1

2

3}, Wen-Ying Guo^{1

2

3}, Jian Sun^{1

2

3}, Ya-Hong Liu^{1

2

3

4}, Xiao-Ping Liao^{5

2

3}

Affiliations

¹ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
² Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
³ Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou, China.
⁴ Jiangsu Co-Innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu, China.
⁵ National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China xpliao@scau.edu.cn.

Abstract

We identified fosA3 at a rate of 2.6% in 310 Salmonella isolates from food animals in Guangdong province, China. The fosA3 gene was genetically linked to diverse antibiotic resistance genes (ARGs), including mcr-1, bla_CTX-M-14/55, oqxAB, and rmtB These gene combinations were embedded in heterogeneous fosA3-containing multidrug resistance regions on the transferable ST3-IncHI2 and F33:A-:B- plasmids and the chromosome. This indicated a great flexibility of fosA3 cotransmission with multiple important ARGs among Salmonella species.

Keywords: IncHI2 plasmids; Salmonella; chromosomally encoded; multidrug resistance; plasmid-mediated fosfomycin resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Drug Resistance, Multiple, Bacterial / genetics*
Fosfomycin / pharmacology*
Plasmids / genetics
Salmonella Infections / epidemiology
Salmonella Infections / microbiology*
Salmonella typhimurium / drug effects
Salmonella typhimurium / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Fosfomycin