Background: Chlorophyllin is used in traditional Chinese medicine because of its anticancer properties. This article describes the preparation and cytotoxic activity of a reduced chlorophyllin derivative (RCD) on tumour cell lines.
Materials and methods: RCD was prepared by reducing chlorophyllin with lithium aluminium hydride, and its solubility in the alkaline and organic phases are different from that of the parent compound chlorophyllin.
Results: RCD also has different chromatographic behaviour from chlorophyllin on thin-layer chromatography and high-pressure liquid chromatography, and excitation and emission spectra. RCD has cytotoxic activity against ZR-75, MCF-7 and HT-29, human tumour cell lines. A clonogenic assay showed that the growth of tumour colonies on soft agar was reduced by RCD in a dose-dependent manner.
Conclusion: RCD is a novel compound exhibiting anticancer activity.
Keywords: Chlorophyllin; RCD; cytotoxic activity; reduced chlorophyllin derivative; reduction; tumour cell lines.
Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.