Characterization of pEncl-30969cz, a novel ColE1-like plasmid encoding VIM-1 carbapenemase, from an Enterobacter cloacae sequence type 92 isolate

Costas C Papagiannitsis; Veronika Paskova; Katerina Chudejova; Matej Medvecky; Ibrahim Bitar; Vladislav Jakubu; Helena Zemlickova; Roman Jirsa; Jaroslav Hrabak

doi:10.1016/j.diagmicrobio.2018.01.024

Characterization of pEncl-30969cz, a novel ColE1-like plasmid encoding VIM-1 carbapenemase, from an Enterobacter cloacae sequence type 92 isolate

Diagn Microbiol Infect Dis. 2018 Jun;91(2):191-193. doi: 10.1016/j.diagmicrobio.2018.01.024. Epub 2018 Feb 2.

Authors

Costas C Papagiannitsis¹, Veronika Paskova², Katerina Chudejova², Matej Medvecky³, Ibrahim Bitar², Vladislav Jakubu⁴, Helena Zemlickova⁵, Roman Jirsa⁶, Jaroslav Hrabak²

Affiliations

¹ Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic. Electronic address: c.papagiannitsis@gmail.com.
² Department of Microbiology, Faculty of Medicine and University Hospital in Pilsen, Charles University, Pilsen, Czech Republic; Biomedical Center, Faculty of Medicine, Charles University, Pilsen, Czech Republic.
³ Veterinary Research Institute, Brno, Czech Republic; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic.
⁴ National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic.
⁵ National Reference Laboratory for Antibiotics, National Institute of Public Health, Prague, Czech Republic; Department of Clinical Microbiology, University Hospital and Faculty of Medicine in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic.
⁶ Department of Clinical Microbiology, Regional Hospital Mlada Boleslav, Mlada Boleslav, Czech Republic.

PMID: 29477275
DOI: 10.1016/j.diagmicrobio.2018.01.024

Abstract

A VIM-1-producing ST92 Enterobacter cloacae was isolated in a Czech hospital. bla_VIM-1 was part of the class 1 integron In110 carried by a Tn1721-like transposon. Tn1721-like was located on a ColE1-like plasmid, pEncl-30969cz (33,003 bp). Target site duplications at the boundaries of Tn1721-like suggested its transposition into the pEncl-30969cz backbone.

Keywords: Class 1 integron; Enterobacteriaceae; In110; Metallo-β-lactamase.

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics
DNA, Bacterial / analysis
DNA, Bacterial / genetics
Enterobacter cloacae / genetics*
Enterobacteriaceae Infections / microbiology*
Humans
Integrons / genetics*
Microbial Sensitivity Tests
Plasmids / genetics*
beta-Lactam Resistance / genetics
beta-Lactamases / genetics

Substances

Anti-Bacterial Agents
Bacterial Proteins
DNA, Bacterial
ROM protein, Bacteria
VIM-1 metallo-beta-lactamase
beta-Lactamases