First Detailed Genetic Characterization of the Structural Organization of Type III Arginine Catabolic Mobile Elements Harbored by Staphylococcus epidermidis by Using Whole-Genome Sequencing

Brenda A McManus; Aoife M O'Connor; Peter M Kinnevey; Michael O'Sullivan; Ioannis Polyzois; David C Coleman

doi:10.1128/AAC.01216-17

First Detailed Genetic Characterization of the Structural Organization of Type III Arginine Catabolic Mobile Elements Harbored by Staphylococcus epidermidis by Using Whole-Genome Sequencing

Antimicrob Agents Chemother. 2017 Sep 22;61(10):e01216-17. doi: 10.1128/AAC.01216-17. Print 2017 Oct.

Authors

Brenda A McManus¹, Aoife M O'Connor¹, Peter M Kinnevey¹, Michael O'Sullivan², Ioannis Polyzois², David C Coleman³

Affiliations

¹ Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Republic of Ireland.
² Division of Restorative Dentistry and Periodontology, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Republic of Ireland.
³ Microbiology Research Unit, Division of Oral Biosciences, Dublin Dental University Hospital, Trinity College Dublin, University of Dublin, Dublin, Republic of Ireland david.coleman@dental.tcd.ie.

Abstract

The type III arginine catabolic mobile element (ACME) was detected in three Staphylococcus epidermidis oral isolates recovered from separate patients (one healthy, one healthy with dental implants, and one with periodontal disease) based on ACME-arc-operon- and ACME-opp3-operon-directed PCR. These isolates were subjected to whole-genome sequencing to characterize the precise structural organization of ACME III for the first time, which also revealed that all three isolates were the same sequence type, ST329.

Keywords: ACME; ST329; Staphylococcus epidermidis; oral cavity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arginine / metabolism
Bacterial Outer Membrane Proteins / genetics
Bacterial Proteins / genetics
Base Sequence
DNA Transposable Elements / genetics*
Genetic Variation
Genome, Bacterial / genetics*
High-Throughput Nucleotide Sequencing
Humans
Membrane Transport Proteins / genetics
Mouth / microbiology
Sequence Analysis, DNA
Staphylococcal Infections / microbiology
Staphylococcus epidermidis / genetics*
Staphylococcus epidermidis / isolation & purification

Substances

Bacterial Outer Membrane Proteins
Bacterial Proteins
CopA protein, Bacteria
DNA Transposable Elements
Membrane Transport Proteins
oligopeptide permease, Bacteria
Arginine