Susceptibility Testing of Common and Uncommon Aspergillus Species against Posaconazole and Other Mold-Active Antifungal Azoles Using the Sensititre Method

Antimicrob Agents Chemother. 2017 May 24;61(6):e00168-17. doi: 10.1128/AAC.00168-17. Print 2017 Jun.

Abstract

We tested 59 common and 27 uncommon Aspergillus species isolates for susceptibility to the mold-active azole antifungal agents itraconazole, voriconazole, and posaconazole using the Sensititre method. The overall essential agreement with the CLSI reference method was 96.5% for itraconazole and posaconazole and was 100% for voriconazole. By the Sensititre method as well as the CLSI reference method, all of 10 A. fumigatus isolates with a cyp51 mutant genotype were classified as being non-wild-type isolates (MIC > epidemiological cutoff value [ECV]) with respect to triazole susceptibility.

Keywords: Aspergillus; Sensititre; antifungal susceptibility testing.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Aspergillosis / drug therapy*
  • Aspergillosis / microbiology
  • Aspergillus / classification
  • Aspergillus / drug effects*
  • Aspergillus / isolation & purification
  • Drug Resistance, Fungal / genetics
  • Itraconazole / pharmacology*
  • Microbial Sensitivity Tests
  • Sterol 14-Demethylase / genetics
  • Triazoles / pharmacology*
  • Voriconazole / pharmacology*

Substances

  • Antifungal Agents
  • Triazoles
  • Itraconazole
  • posaconazole
  • Sterol 14-Demethylase
  • Voriconazole