Expanding the Biologist's Toolkit with CRISPR-Cas9

Samuel H Sternberg; Jennifer A Doudna

doi:10.1016/j.molcel.2015.02.032

Expanding the Biologist's Toolkit with CRISPR-Cas9

Mol Cell. 2015 May 21;58(4):568-74. doi: 10.1016/j.molcel.2015.02.032.

Authors

Samuel H Sternberg¹, Jennifer A Doudna²

Affiliations

¹ Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA.
² Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA; Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA; Innovative Genomics Initiative, University of California, Berkeley, Berkeley, CA 94720, USA; Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address: doudna@berkeley.edu.

PMID: 26000842
DOI: 10.1016/j.molcel.2015.02.032

Abstract

Few discoveries transform a discipline overnight, but biologists today can manipulate cells in ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity mediated by clustered regularly interspaced short palindromic repeats (CRISPR). From elegant studies that deciphered how these immune systems function in bacteria, researchers quickly uncovered the technological potential of Cas9, an RNA-guided DNA cleaving enzyme, for genome engineering. Here we highlight the recent explosion in visionary applications of CRISPR-Cas9 that promises to usher in a new era of biological understanding and control.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
CRISPR-Associated Proteins / genetics
CRISPR-Associated Proteins / metabolism*
CRISPR-Cas Systems / genetics
Clustered Regularly Interspaced Short Palindromic Repeats / genetics*
Deoxyribonuclease I / genetics
Deoxyribonuclease I / metabolism*
Genetic Engineering / methods*
Genome / genetics
Humans
Models, Genetic
RNA, Guide, CRISPR-Cas Systems / genetics
RNA, Guide, CRISPR-Cas Systems / metabolism*

Substances

CRISPR-Associated Proteins
RNA, Guide, CRISPR-Cas Systems
Deoxyribonuclease I

Grants and funding

Howard Hughes Medical Institute/United States