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J Virol. 1995 Apr; 69(4): 2153–2158.
PMCID: PMC188883
PMID: 7884863

Antiviral protection by vesicular stomatitis virus-specific antibodies in alpha/beta interferon receptor-deficient mice.

U Steinhoff, U Müller, A Schertler, H Hengartner, M Aguet, and R M Zinkernagel
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Abstract

The role of innate, alpha/beta interferon (IFN-alpha/beta)-dependent protection versus specific antibody-mediated protection against vesicular stomatitis virus (VSV) was evaluated in IFN-alpha/beta receptor-deficient mice (IFN-alpha/beta R0/0 mice). VSV is a close relative to rabies virus that causes neurological disease in mice. In contrast to normal mice, IFN-alpha/beta R0/0 mice were highly susceptible to infection with VSV because of ubiquitous high viral replication. Adoptive transfer experiments showed that neutralizing antibodies against the glycoprotein of VSV (VSV-G) protected these mice efficiently against systemic infection and against peripheral subcutaneous infection but protected only to a limited degree against intranasal infection with VSV. In contrast, VSV-specific T cells or antibodies specific for the nucleoprotein of VSV (VSV-N) were unable to protect IFN-alpha/beta R0/0 mice against VSV. These results demonstrate that mice are extremely sensitive to VSV if IFN-alpha/beta is not functional and that under these conditions, neutralizing antibody responses mediate efficient protection, but apparently only against extraneuronal infection.

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Selected References

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  • Bachmann MF, Bast C, Hengartner H, Zinkernagel RM. Immunogenicity of a viral model vaccine after different inactivation procedures. Med Microbiol Immunol. 1994 May;183(2):95–104. [PubMed] [Google Scholar]
  • Bachmann MF, Kündig TM, Kalberer CP, Hengartner H, Zinkernagel RM. Formalin inactivation of vesicular stomatitis virus impairs T-cell- but not T-help-independent B-cell responses. J Virol. 1993 Jul;67(7):3917–3922. [PMC free article] [PubMed] [Google Scholar]
  • Cao BN, Huneycutt BS, Gapud CP, Arceci RJ, Reiss CS. Lymphokine expression profile of resting and stimulated CD4+ CTL clones specific for the glycoprotein of vesicular stomatitis virus. Cell Immunol. 1993 Jan;146(1):147–156. [PubMed] [Google Scholar]
  • Dietzschold B, Ertl HC. New developments in the pre- and post-exposure treatment of rabies. Crit Rev Immunol. 1991;10(5):427–439. [PubMed] [Google Scholar]
  • Fu ZF, Dietzschold B, Schumacher CL, Wunner WH, Ertl HC, Koprowski H. Rabies virus nucleoprotein expressed in and purified from insect cells is efficacious as a vaccine. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):2001–2005. [PMC free article] [PubMed] [Google Scholar]
  • Fung-Leung WP, Schilham MW, Rahemtulla A, Kündig TM, Vollenweider M, Potter J, van Ewijk W, Mak TW. CD8 is needed for development of cytotoxic T cells but not helper T cells. Cell. 1991 May 3;65(3):443–449. [PubMed] [Google Scholar]
  • Gobet R, Cerny A, Rüedi E, Hengartner H, Zinkernagel RM. The role of antibodies in natural and acquired resistance of mice to vesicular stomatitis virus. Exp Cell Biol. 1988;56(4):175–180. [PubMed] [Google Scholar]
  • Gresser I, Tovey MG, Bandu ME, Maury C, Brouty-Boyé D. Role of interferon in the pathogenesis of virus diseases in mice as demonstrated by the use of anti-interferon serum. I. Rapid evolution of encephalomyocarditis virus infection. J Exp Med. 1976 Nov 2;144(5):1305–1315. [PMC free article] [PubMed] [Google Scholar]
  • Huneycutt BS, Bi Z, Aoki CJ, Reiss CS. Central neuropathogenesis of vesicular stomatitis virus infection of immunodeficient mice. J Virol. 1993 Nov;67(11):6698–6706. [PMC free article] [PubMed] [Google Scholar]
  • Huneycutt BS, Plakhov IV, Shusterman Z, Bartido SM, Huang A, Reiss CS, Aoki C. Distribution of vesicular stomatitis virus proteins in the brains of BALB/c mice following intranasal inoculation: an immunohistochemical analysis. Brain Res. 1994 Jan 28;635(1-2):81–95. [PubMed] [Google Scholar]
  • Kelley JM, Emerson SU, Wagner RR. The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody. J Virol. 1972 Dec;10(6):1231–1235. [PMC free article] [PubMed] [Google Scholar]
  • Kündig TM, Castelmur I, Bachmann MF, Abraham D, Binder D, Hengartner H, Zinkernagel RM. Fewer protective cytotoxic T-cell epitopes than T-helper-cell epitopes on vesicular stomatitis virus. J Virol. 1993 Jun;67(6):3680–3683. [PMC free article] [PubMed] [Google Scholar]
  • Lefrancois L. Protection against lethal viral infection by neutralizing and nonneutralizing monoclonal antibodies: distinct mechanisms of action in vivo. J Virol. 1984 Jul;51(1):208–214. [PMC free article] [PubMed] [Google Scholar]
  • Lefrancios L, Lyles DS. The interactionof antiody with the major surface glycoprotein of vesicular stomatitis virus. I. Analysis of neutralizing epitopes with monoclonal antibodies. Virology. 1982 Aug;121(1):157–167. [PubMed] [Google Scholar]
  • Leist TP, Cobbold SP, Waldmann H, Aguet M, Zinkernagel RM. Functional analysis of T lymphocyte subsets in antiviral host defense. J Immunol. 1987 Apr 1;138(7):2278–2281. [PubMed] [Google Scholar]
  • Levine B, Hardwick JM, Trapp BD, Crawford TO, Bollinger RC, Griffin DE. Antibody-mediated clearance of alphavirus infection from neurons. Science. 1991 Nov 8;254(5033):856–860. [PubMed] [Google Scholar]
  • Lodmell DL, Esposito JJ, Ewalt LC. Rabies virus antinucleoprotein antibody protects against rabies virus challenge in vivo and inhibits rabies virus replication in vitro. J Virol. 1993 Oct;67(10):6080–6086. [PMC free article] [PubMed] [Google Scholar]
  • Lodmell DL, Sumner JW, Esposito JJ, Bellini WJ, Ewalt LC. Raccoon poxvirus recombinants expressing the rabies virus nucleoprotein protect mice against lethal rabies virus infection. J Virol. 1991 Jun;65(6):3400–3405. [PMC free article] [PubMed] [Google Scholar]
  • Mackett M, Yilma T, Rose JK, Moss B. Vaccinia virus recombinants: expression of VSV genes and protective immunization of mice and cattle. Science. 1985 Jan 25;227(4685):433–435. [PubMed] [Google Scholar]
  • McLAREN LC, HOLLAND JJ, SYVERTON JT. The mammalian cell-virus relationship. I. Attachment of poliovirus to cultivated cells of primate and non-primate origin. J Exp Med. 1959 May 1;109(5):475–485. [PMC free article] [PubMed] [Google Scholar]
  • Miyoshi K, Harter DH, Hsu KC. Neuropathological and immunofluorescence studies of experimental vesicular stomatitis virus encephalitis in mice. J Neuropathol Exp Neurol. 1971 Apr;30(2):266–277. [PubMed] [Google Scholar]
  • Moskophidis D, Frei K, Löhler J, Fontana A, Zinkernagel RM. Production of random classes of immunoglobulins in brain tissue during persistent viral infection paralleled by secretion of interleukin-6 (IL-6) but not IL-4, IL-5, and gamma interferon. J Virol. 1991 Mar;65(3):1364–1369. [PMC free article] [PubMed] [Google Scholar]
  • Moskophidis D, Löhler J, Lehmann-Grube F. Antiviral antibody-producing cells in parenchymatous organs during persistent virus infection. J Exp Med. 1987 Mar 1;165(3):705–719. [PMC free article] [PubMed] [Google Scholar]
  • Müller U, Steinhoff U, Reis LF, Hemmi S, Pavlovic J, Zinkernagel RM, Aguet M. Functional role of type I and type II interferons in antiviral defense. Science. 1994 Jun 24;264(5167):1918–1921. [PubMed] [Google Scholar]
  • Rosenthal KL, Zinkernagel RM. Cross-reactive cytotoxic T cells to serologically distinct vesicular stomatitis virus. J Immunol. 1980 May;124(5):2301–2308. [PubMed] [Google Scholar]
  • Osler AG. Immunology of reaginic allergy: in vitro studies. Clin Exp Immunol. 1970 Jan;6(1):13–23. [PMC free article] [PubMed] [Google Scholar]
  • Wiktor TJ, Macfarlan RI, Reagan KJ, Dietzschold B, Curtis PJ, Wunner WH, Kieny MP, Lathe R, Lecocq JP, Mackett M, et al. Protection from rabies by a vaccinia virus recombinant containing the rabies virus glycoprotein gene. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7194–7198. [PMC free article] [PubMed] [Google Scholar]
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