Caenorhabditis elegans gene ife-2, encoding translation Initiation Factor 4E eIF4E, loss of function of this gene inhibits translation and increases lifespan.
SUMMARY back to top
[Wormbase] ife-2 encodes a translation initiation factor 4F, cap-binding subunit (eIF-4E); by homology, IFE-2 is predicted to function in cap-dependent mRNA translation initiation; loss of ife-2 activity in adult animals extends lifespan.
Wormbase predicts one model

AceView synopsis, each blue text links to tables and details
According to AceView, this gene is expressed at high level, 2.7 times the average gene in this release, only in embryos [Kohara cDNAs]. The expression profile for the gene, derived from the proportion of animals at each stage in each Kohara library is: embryos 100%, L3 to adult 0%. See the in situ hybridization pattern in Kohara NextDB. The sequence of this gene is defined by 20 cDNA clones and 15 elements defined by RNA-seq, some from embryo (seen 7 times). We annotate structural defects or features in one cDNA clone.
The gene contains 3 distinct gt-ag introns. Transcription produces one mRNA. Variant a is transpliced to SL1. There are 2 validated alternative polyadenylation sites (see the diagram).
Function: There are 6 articles specifically referring to this gene in PubMed. In addition we point below to 3 abstracts. This gene is associated to a phenotype (extends adult lifespan). Functionally, the gene has been proposed to participate in a process (translational initiation). Proteins are expected to have molecular functions (RNA binding activity, translation initiation factor activity) and to localize in cytoplasm. This protein appears to interact with another protein (1N443).
The spliced mRNA putatively encodes a good protein, containing Eukaryotic translation initiation factor 4E (eIF-4E) domain [Pfam].

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12.
Map on chromosome X, links to other databases and other names
Map: This gene ife-2 maps on chomosome X at position -19.54 (interpolated). In AceView, it covers 1.36 kb, from 382710 to 381349 (WS190), on the reverse strand.
Links to: WormBase, NextDB, RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name XB2, in Wormbase by its cosmid.number name R04A9.4, in NextDB, the Nematode expression pattern database, as CEYK2255.
Closest AceView homologs in other species ?
The closest human gene, according to BlastP, is the AceView gene IF4E.0 (e=10-35).
The closest mouse gene, according to BlastP, is the AceView gene EG668879andEif4e.
The closest A.thaliana genes, according to BlastP, are the AceView genes EIF4E (e=2 10-28), LSP1 (e=2 10-26), AT1G29550 (e=2 10-25), AT1G29590 (e=7 10-25)
          Complete gene on genome diagram: back to top
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This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones.
Compact gene diagram back to top
Gene ife-2 5' 3' encoded on minus strand of chromosome X from 382,710 to 381,349 a 500bp 0 83 bp exon 83 bp exon 77 bp [gt-ag] intron 13 GenBank accessions 172 bp exon 79 bp [gt-ag] intron 14 GenBank accessions 230 bp exon 334 bp [gt-ag] intron 14 GenBank accessions 387 bp exon 35 accessions, some from embryo (seen 7 times) Transpliced 5' end, 3 accessions Validated 3' end, 42 accessions Validated 3' end, 1 accession 387 bp exon Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Sequences: click on the numbers to get the DNA back to top
mRNA variant mRNA matching the genome Best predicted protein 5' UTR 3' UTR Upstream sequence Transcription
Downstream sequence
a 872 bp 228 aa 6 bp 179 bp 2kb including Promoter 1362 bp 1kb

Gene neighbors and Navigator on chromosome X back to top
wago-12 C I P XB19 R XB23 C XA977 R XA996 D C I P ife-2 C R P XB4 C 5kb 0 sor-3, 25 accessions, 4 variants wago-12, 41 accessions 4 variants XB19, 16 accessions, 4 variants XB23, 7 accessions XA977, 2 accessions XA979, 3 accessions XB21, 1 accession XB8, 0 accession XA996, 29 accessions, 9 variants ife-2, 35 accessions XB4, 17 accessions, 3 variants XB12, 12 accessions, 5 variants ZOOM OUT                 D:disease, C:conserved, I:interactions, R:regulation, P:publications         Read more...
Annotated mRNA diagrams back to top
Bibliography back to top
Please see these 6 articles in PubMed.
In addition we found 3 papers for which we do not have a PubMed identifier
? Gene Summary Gene on genome mRNA Expression Tissue Function, regulation, related genes DCI

To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To see the mRNA diagram, sequence and annotation, click the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.

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