Caenorhabditis elegans gene him-8, high Incidence of Males, encoding HIM-8 like family member.
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Complete gene on genome diagram:
[Wormbase] him-8 encodes a protein with two C-terminal noncanonical C2H2 zinc-fingers whose paralogs include ZIM-1/-3 and C02F5.12; HIM-8 is required by X chromosomes for normal homolog pairing, synapsis, recombination, and segregation during meiosis; him-8 mutants have an increased frequency of genotypically XO males in self-fertile hermaphrodite populations; HIM-8 is expressed during meiosis, and is associated with the X chromosome's meiotic pairing center (PC), which associates with the nuclear envelope during meiotic prophase; him-8 mutations are enhanced by rearrangements that inactivate the X-chromosomal PC; HIM-8 functions are genetically separable, since the him-8(me4) point mutation (which alters a domain N-terminal to HIM-8's zinc fingers) permits normal chromosome binding and nuclear localization, but causes abnormal pairing and synapsis; while the C-terminal region of HIM-8 most closely resembles those of its orthologs in other Caenorhabditis species, its N-terminal region is highly divergent, suggesting species-specific functions; unlike other him mutations, him-8 solely affects X chromosomes, and does not produce embryonic lethality via autosomal nondisjunction or aneuploidy; however, failure of X-chromosomal synapsis in him-8 mutants blocks the pachytene transistion from polarized to nonpolarized meiotic nuclei, by blocking the resolution of recombination intermediates on other chromosomes; him-8-blocked meiotic autosomes show persistent RAD-51 foci and have excess crossovers, both of which may be symptoms of a HUS-1-independent checkpoint induced by X-chromosomal nonsynapsis rather than DNA damage; HIM-8 also acts outside of meiosis, by inhibiting EGL-13 expression or activity; mutations of the HIM-8 zinc-finger domain semidominantly suppress missense (but not null) egl-13 mutations, due to him-8 haploinsufficiency; mutant HIM-8 fails to suppress mutant egl-13 on a free transgenic array, and also fails to suppress native mutant egl-13 if transgenic excess copies of the egl-13 promoter are present.
Map on chromosome IV, links to other databases and other names
This gene him-8 maps on chomosome IV at position +4.85 (interpolated).
Links to: WormBase
The gene is also known in Wormgenes/AceView by its positional name 4L327, in Wormbase by its cosmid.number name T07G12.12.
Compact gene diagram
Sequences: click on the numbers to get the DNA
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line
denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line
denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink
and ] - ] blue
straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags
to any single letter variant of the main . More explanations are given in the gene help file
Summaries of AceView transcripts and proteins
Predicted protein properties
mRNA variant ||
Overview (for structural details see the table below) |
T07G12.12 ((NULL)), mRNA. |
The predicted CDS has 6 exons. It is 1086 bp long and covers 1.90 kb on the WS190 genome. The predicted protein has 361 aa (41.1 kDa, pI 5.9) and a good coding score (4). It contains no protein domain or characteristic Psort motif. BlastP results identify related proteins (threshold .001) in Pseudocoelomata.
The predicted CDS is fully annotated here
Gene neighbors and Navigator on chromosome IV
This table allows to see at a glance from the last column if an isoform has its exonic structure fully supported by a single clone (the variant identifier a, b, c under such mRNA is underlined in the gene diagrams), or if it requires concatenation of two or more cDNA clones (identifier not underlined).
Extends from ||
361 aa |
COOH complete ||
Met (ATG) |
1 to 1086 |
Please see these 10 articles in PubMed
In addition we found 56 papers for which we do not have a PubMed identifier
- [wbg3.1p19] him mutants.
- [wbg6.1p10] CGC news
- [wbg6.1p33] a mutant with decreased X chromosome meiotic non-disjunction.
- [wbg7.2p37] chromosomes in C elegans.
- [wbg8.2p11] screening for recombination-defective mutants.
- [wbg9.1p69] another random walk along the sex determination pathway.
- [wbg9.3p51] a maintenance function for her-1 in control of vitellogenin synthesis and oogenesis in XO adults.
- [wbg10.2p18] more about xol-1: a gene that controls the male mode of sex determination and dosage compensation.
- [wbg10.2p29] molecular cloning of fem-1.
- [wbg10.3p18] update on her-1 transcripts.
- [wbg10.3p62] zinc finger proteins in worms ?
- [wbg10.3p82] embryonic cDNA expression libraries.
- [wbg11.3p28] The cloning and preliminary molecular characterization of sdc-1.
- [wbg11.3p50] Update on Early Embryonic Transcription
- [wbg11.2p74] her-1 transcripts are trans-spliced to SL1
- [wbg11.2p56] ISOLATION AND CHARACTERIZATION OF UNC-104 CDNA CLONES
- [wbg11.2p54] ISOLATION AND CHARACTERIZATION OF UNC-44 CDNA CLONES
- [wbg11.5p72] The effect of him mutations on recombination and segregation.
- [wm91p217] ALTERNATIVE SPLICING AND DEVELOPMENTAL EXPRESSION OF XOL-1 TRANSCRIPTS.
- [wm91p165] Genetic and Molecular Analysis of dpy(y130), a Gene Essential for the Hermapbrodite Mode of Dosage Compensation
- [wm91p64] THE EFFECT OF him-8 ON X-CHROMOSOME SYNAPSIS
- [wbg12.4p53] xol-1 Transcript Analysis and New 'Alleles'
- [wbg12.4p57] Pairing of Homologous Chromosomes Inhibits Intrachromosomal Recombination
- [cgc1810] Trans-generational effects of diethylstilboestrol spermatogenesis and absence of synaptonemal complexes in the him-8 mutant of Caenorhabditis elegans.
- [wbg12.2p68] Promoting her-1
- [wbg12.2p69] Protected Sex: Mapping her-1's Exons
- [wbg12.2p99] Transformation Rescue of him-8
- [wbg13.1p48] The Worm Without a Face: An Update on vab-3
- [wbg13.4p18] The C. elegans cDNA Project: A Progress Report.
- [wbg13.4p45] Possible Involvement of a Predicted RNA-Binding Protein in Primary Sex Determination.
- [wbg13.2p20] Systematic analysis of C. elegans cDNA - Towards an Expression Map of the Genome
- [wbg13.2p43] Molecular Identification of a Major Feminizing Site on the X Chromosome: fox-1
- [wbg14.2p41] MORE EXPRESSION AND OVEREXPRESSION OF A C. ELEGANS TGF-beta HOMOLOGUE, dbl-1
- [wbg14.4p60] Some precious hermaphrodites have gone missing: Transgenic arrays of her-1 V promoter fragments induce dominant sdc phenocopy
- [wcwm96p166] SEEKING A HANDLE ON her-1
- [mwwm96p85] WE CAN'T GET ENOUGH OF HER
- [wbg14.5p62] her-1 strikes back
- [wbg15.1p74] On the trail of two her-1 relatives
- [wbg15.1p72] Are hyp6 and hyp7 interconnected? Some observations from mosaic analyses using SUR-5GFP(NLS)
- [wbg15.1p68] him-8, spontaneous males, and interference
- [wm97e672] ISOLATION OF MUTATIONS IN LRP-1: A CLASSICAL APPROACH TO REVERSE GENETICS
- [wm97e404] HIM-8 AND X-CHROMOSOME MEIOSIS
- [wbg15.3p26] Cloning and Characterization of the Cytokinesis mutant stu-4.
- [ecwm98p134] Genetic and Molecular Analysis of Meiosis
- [wbg15.4p34] Sma-9 a Novel Gene in TGFb Signaling; Something Strange about him-8 Males
- [wbg15.5p19] Strains for making enriched or pure male samples
- [wm99p580] him-5 and him-8
- [wbg16.4p24] Locating spe-6 by mapping the overlap of two LGIII deficiencies, tDf7 and ctDf3
- [mwwm2000p98] Sex-specific gene expression in C. elegans: in search of tra-1 and mab-3 targets
- [euwm2000ab83] Boy is my bursa red: automated detection and sorting of fluorescent stained C. elegans males from a mixed population
- [ecwm2000p68] Functional Characterization of Calcineurin, a serine/threonine protein phosphatase, in C. elegans
- [ecwm2000p89] Boy is my bursa red: automated detection and sorting of fluorescent stained C. elegans males from a mixed population
- [wm2001p71] Specific functions of linker histone isoforms in C. elegans
- [wm2001p290] AIR-2 and CeGLC-7 Function in Chromosome Cohesion in Meiosis
- [wbg16.5p20] Mapping useful GFP insertions; - evidence for local suppression of recombination
- [wm2001p474] him-8 and him-5
To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To see the mRNA diagram, sequence and annotation, click the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.
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