[Wormbase] him-8 encodes a protein with two C-terminal noncanonical C2H2 zinc-fingers whose paralogs include ZIM-1/-3 and C02F5.12; HIM-8 is required by X chromosomes for normal homolog pairing, synapsis, recombination, and segregation during meiosis; him-8 mutants have an increased frequency of genotypically XO males in self-fertile hermaphrodite populations; HIM-8 is expressed during meiosis, and is associated with the X chromosome's meiotic pairing center (PC), which associates with the nuclear envelope during meiotic prophase; him-8 mutations are enhanced by rearrangements that inactivate the X-chromosomal PC; HIM-8 functions are genetically separable, since the him-8(me4) point mutation (which alters a domain N-terminal to HIM-8's zinc fingers) permits normal chromosome binding and nuclear localization, but causes abnormal pairing and synapsis; while the C-terminal region of HIM-8 most closely resembles those of its orthologs in other Caenorhabditis species, its N-terminal region is highly divergent, suggesting species-specific functions; unlike other him mutations, him-8 solely affects X chromosomes, and does not produce embryonic lethality via autosomal nondisjunction or aneuploidy; however, failure of X-chromosomal synapsis in him-8 mutants blocks the pachytene transistion from polarized to nonpolarized meiotic nuclei, by blocking the resolution of recombination intermediates on other chromosomes; him-8-blocked meiotic autosomes show persistent RAD-51 foci and have excess crossovers, both of which may be symptoms of a HUS-1-independent checkpoint induced by X-chromosomal nonsynapsis rather than DNA damage; HIM-8 also acts outside of meiosis, by inhibiting EGL-13 expression or activity; mutations of the HIM-8 zinc-finger domain semidominantly suppress missense (but not null) egl-13 mutations, due to him-8 haploinsufficiency; mutant HIM-8 fails to suppress mutant egl-13 on a free transgenic array, and also fails to suppress native mutant egl-13 if transgenic excess copies of the egl-13 promoter are present. Map on chromosome IV, links to other databases and other names Map: This gene him-8 maps on chomosome IV at position +4.85 (interpolated). Links to:WormBase, RNAiDB. Other names: The gene is also known in Wormgenes/AceView by its positional name 4L327, in Wormbase by its cosmid.number name T07G12.12.
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript. Read more...
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table.
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink and ] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags to any single letter variant of the main . More explanations are given in the gene help file
T07G12.12 ((NULL)), mRNA.
The predicted CDS has 6 exons. It is 1086 bp long and covers 1.90 kb on the WS190 genome. The predicted protein has 361 aa (41.1 kDa, pI 5.9) and a good coding score (4). It contains no protein domain or characteristic Psort motif. BlastP results identify related proteins (threshold .001) in Pseudocoelomata.
This table allows to see at a glance from the last column if an isoform has its exonic structure fully supported by a single clone (the variant identifier a, b, c under such mRNA is underlined in the gene diagrams), or if it requires concatenation of two or more cDNA clones (identifier not underlined).
To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To see the mRNA diagram, sequence and annotation, click the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.
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