Caenorhabditis elegans gene hif-1, hypoxia Inducible Factor, encoding mammalian Hypoxia Inducible Factor homolog, Helix Loop Helix containing protein.
SUMMARY back to top
[Wormbase] hif-1 encodes an ortholog of the mammalian hypoxia-induced factor HIF-1, and is required for survival in hypoxic environments (1% oxygen) that have no effect on wild-type C. elegans.
Wormbase predicts 4 models, but Caenorhabditis elegans cDNA sequences in GenBank, dbEST, Trace and SRA, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 8 spliced variants

AceView synopsis, each blue text links to tables and details
Expression: According to AceView, this gene is expressed at very high level, 9.3 times the average gene in this release, at all stages of development [Kohara cDNAs]. The expression profile for the gene, derived from the proportion of animals at each stage in each Kohara library is: embryos 5%, L1 or L2 larvae 34%, L3 to adult 62%. See the in situ hybridization pattern in Kohara NextDB. The sequence of this gene is defined by 103 cDNA clones and 20 elements defined by RNA-seq, some from mixed (seen 35 times), l1 (34), l2 (14), embryo (5), l4 (5), whole animal (once). We annotate structural defects or features in 3 cDNA clones.
Alternative mRNA variants and regulation: The gene contains 17 distinct gt-ag introns. Transcription produces at least 8 alternatively spliced mRNAs. Variant a is transpliced to SL1, SL2. There are 4 probable alternative promotors, 2 non overlapping alternative last exons and 3 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 4 cassette exons, overlapping exons with different boundaries.
Note that mRNA .d was found in vivo, although it is a predicted target of nonsense mediated mRNA decay (NMD).
Efficacy of translation may be reduced by the presence of a shorter translated product (uORF) initiating at an AUG upstream of the main open reading frame (in variant c).
Function: There are 20 articles specifically referring to this gene in PubMed. In addition we point below to 4 abstracts. This gene is associated to a phenotype (adaptation to hypoxia, Hypoxia Inducible Factor, reduced susceptibility to pathogens). Functionally, the gene has been proposed to participate in a process (regulation of transcription). Proteins are expected to have molecular function (transcription regulator activity) and to localize in nucleus. These proteins appear to interact with other proteins (AHA-1, CBP-1, EGL-9, LIN-2, VHL-1). The gene interacts with MTSS-1.
Protein coding potential: The 8 spliced mRNAs putatively encode good proteins, altogether 8 different isoforms (3 complete, 1 COOH complete, 4 partial), some containing domains Basic helix-loop-helix dimerisation region bHLH, PAS fold-3 [Pfam].

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12.
Map on chromosome V, links to other databases and other names
Map: This gene hif-1 maps on chomosome V at position +29.88 (interpolated). In AceView, it covers 4.88 kb, from 20775801 to 20780680 (WS190), on the direct strand.
Links to: WormBase, NextDB, RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 5V253, in Wormbase by its cosmid.number name F38A6.3, in NextDB, the Nematode expression pattern database, as CEYK179.
Closest AceView homologs in other species ?
The closest human gene, according to BlastP, is the AceView gene HIF3A (e=10-11).
The closest mouse genes, according to BlastP, are the AceView genes Hif3a (e=3 10-39), Epas1 (e=2 10-35)
          Complete gene on genome diagram: back to top
Please choose between the zoomable GIF version., and the Flash version.
This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones.
Compact gene diagram back to top
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Sequences: click on the numbers to get the DNA back to top
mRNA variant mRNA matching the genome Best predicted protein 5' UTR 3' UTR uORF Upstream sequence Transcription
Downstream sequence
a 2680 bp 719 aa 89 bp 431 bp 2kb including Promoter 4880 bp 1kb
b 2843 bp 694 aa 756 bp 2kb probably including promoter 4840 bp 1kb
c 1978 bp 505 aa 64 bp 396 bp 36 bp 2kb including Promoter 2786 bp 1kb
d 2311 bp 433 aa 258 bp 751 bp 2kb probably including promoter 3403 bp 1kb
e 514 bp 170 aa 2kb 713 bp 1kb
f 490 bp 162 aa 2kb 713 bp 1kb
g 385 bp 107 aa 62 bp 2kb probably including promoter 717 bp 1kb
h 141 bp 46 aa 2kb 485 bp 1kb

Gene neighbors and Navigator on chromosome V back to top
ZOOM OUT                 D:disease, C:conserved, I:interactions, R:regulation, P:publications        
Annotated mRNA diagrams back to top
Bibliography back to top
Please see these 20 articles in PubMed.
In addition we found 4 papers for which we do not have a PubMed identifier
? Gene Summary Gene on genome mRNA:.a, .b, .c, .d, .e, .f, .g, .h Alternative mRNAs features, proteins, introns, exons, sequences Expression Tissue Function, regulation, related genes DCI

To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To compare alternative variants, their summarized annotations, predicted proteins, introns and exons, or to access any sequence, click the 'Alternative mRNAs features' tab. To see a specific mRNA variant diagram, sequence and annotation, click the variant name in the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.

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