[Wormbase] her-1 generates two transcripts, the larger of which encodes a small, secreted, cysteine-rich protein (HER-1A) required for normal sex determination in wild-type XO male animals, while being dispensable for sex determination in XX hermaphrodites; transcription of her-1 in XX animals is repressed by SDC-2; HER-1A protein has two all-helical domains, with fourteen conserved cysteine residues forming seven disulfide bonds, at least eight of which residues are critical for function in vivo; HER-1A has no known homologs except in other nematodes (e.g., Brugia malayi), and does not structurally resemble other well-known secreted proteins; HER-1A inhibits the receptor TRA-2A in vivo, and binds it in vitro; HER-1A functions nonautonomously and requires a signal sequence for biological activity; the her-1(n695gf) mutation alters a site in the her-1 promoter bound by SDC-2, partially transforms XX animals into phenotypic males, and requires sup-26 and sup-27 to do so.
Wormbase predicts 2 models.
AceView synopsis, each blue text links to tables and details
According to AceView, this gene is well expressed, 0.5 times the average gene in this release. The sequence of this gene is defined by 5 cDNA clones and one element defined by RNA-seq.
The gene contains 3 distinct gt-ag introns. Transcription produces one mRNA. Function: There are 26 articles specifically referring to this gene in PubMed. In addition we point below to 107 abstracts. This gene is associated to a phenotype (HERmaphrodization of XO animals). The gene interacts with 12 other genes (DPY-21, DPY-30, FEM-3, FOX-1, SDC-1, SDC-2, SDC-3, SEL-10, TRA-1, TRA-2, TRA-3, XOL-1).
The spliced mRNA putatively encodes a good protein, some containing Protein of unknown function Her-1 domain [Pfam].
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript. Read more...
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table.
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink and ] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags to any single letter variant of the main . More explanations are given in the gene help file
To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To see the mRNA diagram, sequence and annotation, click the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.
If you know more about this gene, or found errors, please share your knowledge. Thank you !