Caenorhabditis elegans gene gst-1, encoding glutathione S-Transferase, P subunit.
SUMMARY back to top
[Wormbase] gst-1 encodes a putative glutathione S-transferase of the pi class; GST-1 is required for sperm to normally migrate towards a PUFA-based signal exuded by oocytes; gst-1(RNAi) hermaphrodites are infertile, both through aberrant loss of their own sperm and through failure of males to effectively inseminate them.
Wormbase predicts one model, but Caenorhabditis elegans cDNA sequences in GenBank, dbEST, Trace and SRA, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 4 spliced variants

AceView synopsis, each blue text links to tables and details
Expression: According to AceView, this gene is expressed at very high level, 4.3 times the average gene in this release, mostly in embryos, and some at all stages of development [Kohara cDNAs]. The expression profile for the gene, derived from the proportion of animals at each stage in each Kohara library is: embryos 34%, L1 or L2 larvae 21%, L3 to adult (including dauer) 45%. See the in situ hybridization pattern in Kohara NextDB. The sequence of this gene is defined by 29 cDNA clones and 27 elements defined by RNA-seq, some from embryo (seen 7 times), mixed (4), l1 (3), whole worm (2), l2 (once). We annotate structural defects or features in one cDNA clone.
Alternative mRNA variants and regulation: The gene contains 11 distinct gt-ag introns. Transcription produces at least 5 different mRNAs, 4 alternatively spliced variants and 1 unspliced form. cls-2.a is transpliced to SL1cls-2.b to SL1, SL2, SL3, SL4, SL5, SL7, SL8cls-2.e to SL1. There are 2 validated alternative polyadenylation sites (see the diagram). 504 bp of this gene are antisense to spliced gene lin-12, raising the possibility of regulated alternate expression.
Function: There are 4 articles specifically referring to this gene in PubMed. In addition we point below to 3 abstracts. Proteins are expected to localize in cytoplasm. These proteins appear to interact with another protein (GST-23).
Protein coding potential: 4 spliced mRNAs putatively encode good proteins, altogether 4 different isoforms (3 complete, 1 partial), some containing domains glutathione S-transferase, C-terminal, Glutathione S-transferase, N-terminal, HEAT [Pfam], a coiled coil stretch [Psort2]. The remaining mRNA variant (unspliced; partial) appears not to encode a good protein.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12.
Map on chromosome III, links to other databases and other names
Map: This gene gst-1 maps on chomosome III at position +0.08 (interpolated). In AceView, it covers 5.14 kb, from 9054962 to 9060098 (WS190), on the direct strand.
Links to: WormBase, NextDB, RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 3K149, in Wormbase by its cosmid.number name R107.7, in NextDB, the Nematode expression pattern database, as CEYK3081.
Closest AceView homologs in other species ?
The closest human genes, according to BlastP, are the AceView genes CLASP2 (e=7 10-36), CLASP1 (e=9 10-36).
The closest mouse gene, according to BlastP, is the AceView gene Clasp2 (e=2 10-36).
The closest A.thaliana gene, according to BlastP, is the AceView gene ATCLASP\/CLASP (e=9 10-15)
          Complete gene on genome diagram: back to top
Please choose between the zoomable GIF version., and the HTML5/SVG version.
This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones.
Compact gene diagram back to top
Gene gst-1 5' 3' encoded on plus strand of chromosome III from 9,059,242 to 9,060,098 l l l l l 1 2kb 0 93 bp exon 49 bp [gt-ag] intron 4 GenBank accessions 802 bp exon 45 bp [gt-ag] intron 3 GenBank accessions 656 bp exon 47 bp [gt-ag] intron 2 GenBank accessions 384 bp exon 44 bp [gt-ag] intron 2 GenBank accessions 384 bp exon 225 bp [gt-ag] intron 2 GenBank accessions 441 bp exon 51 bp [gt-ag] intron 5 GenBank accessions 120 bp exon 51 bp [gt-ag] intron 6 GenBank accessions 451 bp exon 18 accessions, some from mixed (seen 3 times) embryo (2), l1 (2), whole worm (once) Transpliced 5' end, 3 accessions Validated 3' end, 4 accessions 451 bp exon 161 bp exon 161 bp exon 54 bp [gt-ag] intron 16 GenBank accessions 88 bp exon 48 bp [gt-ag] intron 15 GenBank accessions 504 bp exon 34 accessions, some from embryo (seen 5 times) l1 (once), l2 (once) mixed (once), whole worm (once) Transpliced 5' end, 5 accessions Validated 3' end, 95 accessions 504 bp exon 441 bp exon 42 bp [gt-ag] intron 1 GenBank accession 1 accession 129 bp exon 154 bp exon 2 accessions Transpliced 5' end, 1 accession 154 bp exon 507 bp exon 507 bp exon 507 bp exon 374 bp [gt-ag] intron 1 GenBank accession 158 bp exon 158 bp exon 1 accession 158 bp exon Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Sequences: click on the numbers to get the DNA back to top
mRNA variant mRNA matching the genome Best predicted protein 5' UTR 3' UTR Upstream sequence Transcription
Downstream sequence
a 3331 bp 1020 aa 268 bp 2kb including Promoter 3843 bp 1kb
b 753 bp 208 aa 26 bp 100 bp 2kb including Promoter 855 bp 1kb
c 570 bp 190 aa 2kb 612 bp 1kb
d 665 bp 75 aa 349 bp 88 bp 2kb possibly including promoter 1039 bp 1kb
e 154 bp 44 aa 22 bp 2kb including Promoter 154 bp 1kb

Gene neighbors and Navigator on chromosome III back to top
cls-2 D C I P gst-1 C I P far-2 D C I P C R P nac-2 C 3K134 C I R P ikke-1 C I R P 3K142 D C I R P lin-12 C 3K164 D C R P far-1 5kb 0 cls-2, 56 accessions, 5 variants gst-1, 56 accessions, 5 variants far-2, 226 accessions 4 variants 3K162, 4 accessions nac-2, 70 accessions, 5 variants 3K134, 12 accessions, 2 variants ikke-1, 50 accessions 7 variants 3K142, 87 accessions, 5 variants lin-12, 21 accessions 2 variants 3K164, 5 accessions far-1, 212 accessions 6 variants ZOOM OUT                 D:disease, C:conserved, I:interactions, R:regulation, P:publications         Read more...
Annotated mRNA diagrams back to top
Bibliography back to top
Please see these 4 articles in PubMed.
In addition we found 3 papers for which we do not have a PubMed identifier
? Gene Summary Gene on genome mRNA:cl, cl, cl, cl, cl Alternative mRNAs features, proteins, introns, exons, sequences Expression Tissue Function, regulation, related genes CI

To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To compare alternative variants, their summarized annotations, predicted proteins, introns and exons, or to access any sequence, click the 'Alternative mRNAs features' tab. To see a specific mRNA variant diagram, sequence and annotation, click the variant name in the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.

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