[Wormbase] The cua-1 gene encodes a putative E1-E2 ATPase orthologous to the human gene KIAA1347 (ATP7A; OMIM:604384), which when mutated leads to Hailey-Hailey disease.
Wormbase predicts one model, but Caenorhabditis elegans cDNA sequences in GenBank, dbEST, Trace and SRA, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 8 spliced variants.
AceView synopsis, each blue text links to tables and details Expression: According to AceView, this gene is expressed at very high level, 21.0 times the average gene in this release, at all stages of development [Kohara cDNAs]. The expression profile for the gene, derived from the proportion of animals at each stage in each Kohara library is: embryos 9%, L1 or L2 larvae 59%, L3 to adult 32%. See the in situ hybridization pattern in Kohara NextDB. The sequence of this gene is defined by 238 cDNA clones and 38 elements defined by RNA-seq, some from l1 (seen 128 times), l2 (38), mixed (24), embryo (18), l4 (11), whole animal (2). We annotate structural defects or features in 12 cDNA clones. Alternative mRNA variants and regulation: The gene contains 28 distinct gt-ag introns. Transcription produces at least 9 different mRNAs, 8 alternatively spliced variants and 1 unspliced form. Variant a is transpliced to SL1, SL2, SL3, SL4, b to SL2, SL1, SL1', i to SL1. There are 3 probable alternative promotors, 7 non overlapping alternative last exons and 18 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 6 cassette exons, overlapping exons with different boundaries. 155 bp of this gene are antisense to spliced gene 3O511, raising the possibility of regulated alternate expression.
2 variants were isolated in vivo, despite the fact that they are predicted targets of nonsense mediated mRNA decay (NMD). Function: There are 5 articles specifically referring to this gene in PubMed. In addition we point below to 4 abstracts. This essential gene is associated to a phenotype (Paralysed, UNCoordinated locomotion, affects axonal growth or guidance). Functionally, the gene has been proposed to participate in processes (metabolism, metal ion transport). Proteins are expected to have molecular functions (ATP binding activity, enzyme activity, hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances, metal ion binding activity) and to localize in various compartments (cytoplasm, membrane, plasma membrane). Protein coding potential: 6 spliced mRNAs putatively encode good proteins, altogether 6 different isoforms (3 complete, 3 COOH complete), some containing domains ABC transporter-like, E1-E2 ATPase-associated region, Heavy metal transport/detoxification protein, haloacid dehalogenase-like hydrolase [Pfam], some transmembrane domains, a second peroximal domain, a coiled coil stretch [Psort2]. The remaining 3 mRNA variants (2 spliced, 1 unspliced; 3 partial) appear not to encode good proteins.
Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12. Map on chromosome III, links to other databases and other names Map: This essential gene cua-1 maps on chomosome III at position +20.97 (interpolated). In AceView, it covers 44.59 kb, from 13467699 to 13423111 (WS190), on the reverse strand. Links to:WormBase, NextDB, RNAiDB. Other names: The gene is also known in Wormgenes/AceView by its positional name 3O530, in Wormbase by its cosmid.number name Y76A2A.2, in NextDB, the Nematode expression pattern database, as CEYK618. Closest AceView homologs in other species ? The closest human genes, according to BlastP, are the AceView genes ATP7B (e=2 10-08), ATP7A (e=5 10-07), LOC644732 (e=6 10-07). The closest mouse genes, according to BlastP, are the AceView genes Atp7b (e=2 10-08), Atp7a (e=2 10-07). The closest A.thaliana genes, according to BlastP, are the AceView genes HMA5 (e=4 10-07), PAA1.1 (e=2 10-05)
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript. Read more...
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table.
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink and ] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags to any single letter variant of the main . More explanations are given in the gene help file
To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To compare alternative variants, their summarized annotations, predicted proteins, introns and exons, or to access any sequence, click the 'Alternative mRNAs features' tab. To see a specific mRNA variant diagram, sequence and annotation, click the variant name in the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.
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