[Wormbase] arx-7 encodes the C. elegans ortholog of the p16Arc subunit of the actin-related protein (Arp)2/3 complex.
Wormbase predicts 4 models, but Caenorhabditis elegans cDNA sequences in GenBank, dbEST, Trace and SRA, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 12 spliced variants
AceView synopsis, each blue text links to tables and details
According to AceView, this gene is expressed at very high level
, 10.0 times the average gene in this release, at all stages of development [Kohara cDNAs]. The expression profile for the gene, derived from the proportion of animals at each stage in each Kohara library is: embryos 17%, L1 or L2 larvae 27%, L3 to adult 56%. See the in situ hybridization pattern in Kohara NextDB
. The sequence
of this gene is defined by 57 cDNA clones
and 75 elements defined by RNA-seq, some from l1 (seen 11 times), mixed (11), embryo (9), l2 (3), l4 (3). We annotate structural defects or features
in 2 cDNA clones.
Alternative mRNA variants and regulation:
The gene contains 23 distinct introns
(22 gt-ag, 1 gc-ag). Transcription produces at least 13 different mRNAs
, 12 alternatively spliced variants and 1 unspliced form. Variant a is transpliced to SL2, b to SL2, SL9, c to SL3, SL2, SL1, SL4, j to SL1, SL2, SL3. There are 3 probable alternative promotors
, 4 non overlapping alternative last exons and 13 validated alternative polyadenylation sites
(see the diagram
). The mRNAs appear to differ by truncation of the 5' end, truncation of the 3' end, presence or absence of 6 cassette exons
, overlapping exons with different boundaries, splicing versus retention of 2 introns. 427 bp of this gene are antisense to spliced gene 1D637
, raising the possibility of regulated alternate expression.
2 variants were isolated in vivo
, despite the fact that they are predicted targets of nonsense mediated mRNA decay
There are 2 articles
specifically referring to this gene in PubMed. This essential gene is associated to a phenotype
(Embryonic Lethal, Larval arrest, unhealthy, defective hypodermal enclosure). Functionally, the gene has been proposed to participate in a process
(regulation of actin polymerization). Proteins are expected to localize
in various compartments (nucleus, cytoskeleton).
Protein coding potential:
10 spliced mRNAs putatively encode good proteins
, altogether 10 different isoforms (3 complete, 3 COOH complete, 4 partial
), some containing ARP2/3 complex 16 kDa subunit (p16-Arc) domain
[Pfam], a coiled coil stretch [Psort2]
. The remaining 3 mRNA variants (2 spliced, 1 unspliced; 3 partial) appear not to encode good proteins.
Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12
Map on chromosome I, links to other databases and other names
This essential gene arx-7 maps on chomosome I at position -4.88 (interpolated). In AceView, it covers 15.56 kb
, from 3084875 to 3069316 (WS190), on the reverse strand.
Links to: WormBase
The gene is also known in Wormgenes/AceView by its positional name 1D638, in Wormbase by its cosmid.number name M01B12.3, M01B12.4, in NextDB, the Nematode expression pattern database, as CEYK2106.
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line
denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line
denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink
and ] - ] blue
straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags
to any single letter variant of the main . More explanations are given in the gene help file