Caenorhabditis elegans gene ace-4, abnormal ACEtylcholinesterase, encoding acetylcholinesterase class C, serine hydrolase.
SUMMARY back to top
The gene ace-4 encodes a rare form of acetylcholinesterase class C, closer to ACE-3 (with which it forms a direct tandem repeat) than ACE-1 or ACE-2 by sequence [Grauso et al, 1998; Combes et al, 2000]. Its activity is not detected in vivo. The usual sequence FGESAG surrounding the active serine residue in cholinesterases is changed to FGQSAG. ace-4 is expressed at least in L2 larvae [Kohara cDNA][Wormbase] ace-4 encodes an divergent acetylcholinesterase homolog with biochemically undetectable activity.
Wormbase predicts one model

AceView synopsis, each blue text links to tables and details
According to AceView, this gene is expressed at high level, 1.7 times the average gene in this release. The sequence of this gene is defined by 4 cDNA clones and 18 elements defined by RNA-seq, some from l2 (seen once). We annotate structural defects or features in one cDNA clone.
The gene contains 16 distinct gt-ag introns. Transcription produces one mRNA. Variant a is transpliced to SL1.
Function: There are 6 articles specifically referring to this gene in PubMed. In addition we point below to one abstract. This gene is associated to a phenotype (abnormal ACEtylcholinesterase). Proteins are expected to localize in endoplasmic reticulum.
The spliced mRNA putatively encodes a good protein, containing carboxylesterase, type B domain [Pfam].

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12.
Map on chromosome II, links to other databases and other names
Map: This gene ace-4 maps on chomosome II at position +23.57 (interpolated). In AceView, it covers 7.41 kb, from 14197949 to 14205356 (WS190), on the direct strand.
Links to: WormBase, RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 2O495, in Wormbase by its cosmid.number name Y48B6A.7.
Closest AceView homologs in other species ?
The closest human genes, according to BlastP, are the AceView genes BCHE, ACHE.
The closest mouse genes, according to BlastP, are the AceView genes Bche (e=5 10-98), Acheand2700038N03Rik (e=10-88).
The closest A.thaliana genes, according to BlastP, are the AceView genes AT3G02410 (e=0.006), ATPCME (e=0.015), AT1G26120 (e=0.026)
          Complete gene on genome diagram: back to top
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This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones.
Compact gene diagram back to top
Gene ace-4 5' 3' encoded on plus strand of chromosome II from 14,197,949 to 14,205,356 a 1 2kb 0 172 bp exon 172 bp exon 609 bp [gt-ag] intron 5 GenBank accessions 107 bp exon 54 bp [gt-ag] intron 5 GenBank accessions 222 bp exon 48 bp [gt-ag] intron 5 GenBank accessions 79 bp exon 971 bp [gt-ag] intron 5 GenBank accessions 117 bp exon 382 bp [gt-ag] intron 5 GenBank accessions 60 bp exon 57 bp [gt-ag] intron 4 GenBank accessions 71 bp exon 46 bp [gt-ag] intron 3 GenBank accessions 124 bp exon 462 bp [gt-ag] intron 2 GenBank accessions 110 bp exon 67 bp [gt-ag] intron 2 GenBank accessions 101 bp exon 136 bp [gt-ag] intron 2 GenBank accessions 67 bp exon 1414 bp [gt-ag] intron 2 GenBank accessions 193 bp exon 77 bp [gt-ag] intron 4 GenBank accessions 86 bp exon 817 bp [gt-ag] intron 4 GenBank accessions 156 bp exon 100 bp [gt-ag] intron 4 GenBank accessions 69 bp exon 48 bp [gt-ag] intron 4 GenBank accessions 107 bp exon 62 bp [gt-ag] intron 4 GenBank accessions 217 bp exon 22 accessions, some from l2 (seen once) Transpliced 5' end, 2 accessions Validated 3' end, 65 accessions 217 bp exon Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
Sequences: click on the numbers to get the DNA back to top
mRNA variant mRNA matching the genome Best predicted protein 5' UTR 3' UTR Upstream sequence Transcription
Downstream sequence
a 2058 bp 602 aa 121 bp 128 bp 2kb including Promoter 7408 bp 1kb

Gene neighbors and Navigator on chromosome II back to top
2O479 C R ace-4 D C P ace-3 D C I P 2O505 C hot-7 R 2O498 D C P 2O512 5kb 0 2O479, 51 accessions, 5 variants ace-4, 22 accessions ace-3, 16 accessions, 2 variants 2O505, 8 accessions, 3 variants hot-7, 5 accessions 2O504, 3 accessions, 2 variants 2O506, 7 accessions 2O498, 1 accession 2O512, 19 accessions, 3 variants ZOOM OUT                 D:disease, C:conserved, I:interactions, R:regulation, P:publications         Read more...
Annotated mRNA diagrams back to top
Bibliography back to top
Please see these 6 articles in PubMed.
In addition we found 1 papers for which we do not have a PubMed identifier
? Gene Summary Gene on genome mRNA Expression Tissue Function, regulation, related genes DC

To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To see the mRNA diagram, sequence and annotation, click the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.

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