Caenorhabditis elegans gene ace-2, abnormal ACEtylcholinesterase, encoding acetylcholinesterase class B, associates into glycolipid-anchored dimers.
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SUMMARY back to top
Summary
The gene ace-2 encodes acetylcholinesterase class B, and produces about 45% of the nematode acetylcholinesterase activity [Johnson and Russell, 1983]. The C terminus of ACE-2 is hydrophobic, of H type; it associates into glycolipid-anchored dimers [Combes et al, 2000]. ace-2 is partly redundant functionnally with ace-1: the double mutant ace-1 ace-2 has uncoordinated locomotion, moving slowly forward and hypercontracted backward [Kolson and Russell, 1985]. Absence of the three classes A B C of acetylcholinesterases together, in the ace-1 ace-2 ace-3 triple mutant, leads to paralysis and late embryonic/early larval lethality [Johnson et al, 1988]. ace-2 is expressed at low level in larvae, in overlap with ace-1, but also specifically in the pharyngeo-intestinal valve where ace-1 is not expressed [Combes et al, 2000][Wormbase] An acetylcholineesterase involved in the termination of cholinergic nerve transmission; it is predominantly expressed in motoneurons.
Wormbase predicts one model, but Caenorhabditis elegans cDNA sequences in GenBank, dbEST, Trace and SRA, filtered against clone rearrangements, coaligned on the genome and clustered in a minimal non-redundant way by the manually supervised AceView program, support at least 4 spliced variants
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AceView synopsis, each blue text links to tables and details
Expression: According to AceView, this gene is expressed at high level, 1.4 times the average gene in this release, in L1, L2 and L3 larvae [Kohara cDNAs]. The sequence of this gene is defined by 5 cDNA clones and 14 elements defined by RNA-seq, some from l1 (seen once), l2 (once). We annotate structural defects or features in one cDNA clone.
Alternative mRNA variants and regulation: The gene contains 11 distinct introns (10 gt-ag, 1 gc-ag). Transcription produces 4 alternatively spliced mRNAs. Variant a is transpliced to SL1. There are 3 probable alternative promotors, 2 non overlapping alternative last exons and 4 validated alternative polyadenylation sites (see the diagram). The mRNAs appear to differ by truncation of the 3' end.
Function: There are 21 articles specifically referring to this gene in PubMed. In addition we point below to 21 abstracts. This gene is associated to a phenotype (abnormal ACEtylcholinesterase). Proteins are expected to localize in membrane. The gene interacts with 4 other genes (ACE-1, ACE-3, DYS-1, UNC-17+CHA-1).
Protein coding potential: 2 spliced mRNAs putatively encode good proteins, altogether 2 different isoforms (1 complete, 1 COOH complete), some containing carboxylesterase, type B domain [Pfam], some transmembrane domains [Psort2]. The remaining 2 mRNA variants (2 spliced; 1 partial) appear not to encode good proteins.

Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12.
Map on chromosome I, links to other databases and other names
Map: This gene ace-2 maps on chomosome I at position -4.04 (interpolated). In AceView, it covers 8.55 kb, from 3309919 to 3301371 (WS190), on the reverse strand.
Links to: WormBase, RNAiDB.
Other names: The gene is also known in Wormgenes/AceView by its positional name 1D872, in Wormbase by its cosmid.number name Y44E3A.2.
Closest AceView homologs in other species ?
The closest human gene, according to BlastP, is the AceView gene BCHE (e=8 10-99).
The closest mouse genes, according to BlastP, are the AceView genes Bche, Acheand2700038N03Rik.
The closest A.thaliana genes, according to BlastP, are the AceView genes AT3G02410, ATPCME, AT5G47000
          Complete gene on genome diagram: back to top
Please choose between the zoomable GIF version., and the Flash version.
This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones.
Compact gene diagram back to top
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript.
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Sequences: click on the numbers to get the DNA back to top
mRNA variant mRNA matching the genome Best predicted protein 5' UTR 3' UTR uORF Upstream sequence Transcription
unit
pre-mRNA
Downstream sequence
a 2837 bp 629 aa 120 bp 827 bp 63 bp 2kb including Promoter 6216 bp 1kb
b 405 bp 91 aa 129 bp 2kb 1279 bp 1kb
c 495 bp 58 aa 318 bp 2kb 699 bp 1kb
d 457 bp 31 aa 114 bp 247 bp 2kb 2609 bp 1kb

Gene neighbors and Navigator on chromosome I back to top
ZOOM OUT                 D:disease, C:conserved, I:interactions, R:regulation, P:publications         Read more...
Annotated mRNA diagrams back to top
Bibliography back to top
Please see these 21 articles in PubMed.
In addition we found 21 papers for which we do not have a PubMed identifier
? Gene Summary Gene on genome mRNA:.a, .b, .c, .d Alternative mRNAs features, proteins, introns, exons, sequences Expression Tissue Function, regulation, related genes DCI

To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To compare alternative variants, their summarized annotations, predicted proteins, introns and exons, or to access any sequence, click the 'Alternative mRNAs features' tab. To see a specific mRNA variant diagram, sequence and annotation, click the variant name in the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.

If you know more about this gene, or found errors, please share your knowledge. Thank you !