U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    raf1 Rik1-associated factor Raf1 [ Schizosaccharomyces pombe (fission yeast) ]

    Gene ID: 2538848, updated on 11-Apr-2024

    Summary

    Go to the top of the page Help
    Gene symbol
    raf1
    Gene description
    Rik1-associated factor Raf1
    Primary source
    PomBase:SPCC613.12c
    Locus tag
    SPCC613.12c
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Schizosaccharomyces pombe (strain: 972h-)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
    Also known as
    clr8; cmc1; dos1
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Location:
    chromosome: III
    Exon count:
    1
    Sequence:
    Chromosome: III; NC_003421.2 (99716..102315, complement)

    Chromosome III - NC_003421.2Genomic Context describing neighboring genes Neighboring gene putative tRNA-splicing endonuclease subunit Sen54 Neighboring gene putative ubiquinol-cytochrome-c reductase complex core protein Qcr2 Neighboring gene protein meu23 Neighboring gene miscRNA Neighboring gene ATP-dependent helicase Neighboring gene DNA repair protein Neighboring gene miscRNA Neighboring gene uncharacterized protein

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_003421.2

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. Lid2 is required for coordinating H3K4 and H3K9 methylation of heterochromatin and euchromatin. Li F, et al. Cell, 2008 Oct 17. PMID 18957202, Free PMC Article
    2. Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex. Gonzalez M, et al. Proc Natl Acad Sci U S A, 2013 Jan 8. PMID 23267073, Free PMC Article
    3. The Clr7 and Clr8 directionality factors and the Pcu4 cullin mediate heterochromatin formation in the fission yeast Schizosaccharomyces pombe. Thon G, et al. Genetics, 2005 Dec. PMID 16157682, Free PMC Article
    4. Two novel proteins, dos1 and dos2, interact with rik1 to regulate heterochromatic RNA interference and histone modification. Li F, et al. Curr Biol, 2005 Aug 23. PMID 16040243
    5. CRL4-like Clr4 complex in Schizosaccharomyces pombe depends on an exposed surface of Dos1 for heterochromatin silencing. Kuscu C, et al. Proc Natl Acad Sci U S A, 2014 Feb 4. PMID 24449894, Free PMC Article

    See all (25) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Disruption of Dos1, Dos2, or Cdc20 results in dissociation of CENP-A from centromeres and mislocalization of the protein to noncentromeric sites during S phase.
      Title: Cell cycle-dependent deposition of CENP-A requires the Dos1/2-Cdc20 complex.
    2. Thus the association of a DCAF, Raf1, with its adaptor, Rik1, is required for histone methylation and to allow RNAi to signal to chromatin
      Title: Raf1 Is a DCAF for the Rik1 DDB1-like protein and has separable roles in siRNA generation and chromatin modification.
    3. Dos1 acts in the RNAi-dependent heterochromatin pathway. It is required for silencing of heterochromatin regions, for H3K9me association with centromeric repeats, for production of siRNAs, and for Swi6 localization. It directly interacts with Rik1. [dos1]
      Title: Two novel proteins, dos1 and dos2, interact with rik1 to regulate heterochromatic RNA interference and histone modification.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by PomBase

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in CENP-A containing chromatin assembly IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in meiotic telomere clustering IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in siRNA-mediated pericentric heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in silent mating-type cassette heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in subtelomeric heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of CLRC complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of chromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    is_active_in cytosol HDA PubMed 
    part_of mating-type region heterochromatin TAS
    Traceable Author Statement
    more info
    		 PubMed 
    is_active_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    is_active_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of pericentric heterochromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

    Go to the top of the page Help
    Preferred Names
    Rik1-associated factor Raf1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_003421.2 Reference assembly

      Range
      99716..102315 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001022695.2 → NP_587700.1  Rik1-associated factor Raf1 [Schizosaccharomyces pombe]

      See identical proteins and their annotated locations for NP_587700.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      O74910
      Conserved Domains (3) summary
      COG2319
      Location:300 → 622
      WD40; WD40 repeat [General function prediction only]
      sd00039
      Location:302 → 338
      7WD40; WD40 repeat [structural motif]
      cl02567
      Location:302 → 607
      WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.