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    topB DNA topoisomerase III [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946141, updated on 4-Apr-2024

    Summary

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    Gene symbol
    topB
    Gene description
    DNA topoisomerase III
    Primary source
    ASAP:ABE-0005869
    Locus tag
    b1763
    See related
    ECOCYC:EG11014
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1761; mutR
    Summary
    DNA topoisomerase III (Topo III) is a type I topoisomerase which can decatenate DNA; at high temperatures in vitro, the enzyme can also relax negative supercoils in DNA . [More information is available at EcoCyc: EG11014].
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    Genomic context

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    Sequence:
    NC_000913.3 (1844999..1846960, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene glutamate dehydrogenase Neighboring gene DUF1266 domain-containing protein YnjI Neighboring gene selenide, water dikinase Neighboring gene putative oxidoreductase YdjA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Topoisomerase III Acts at the Replication Fork To Remove Precatenanes. Lee CM, et al. J Bacteriol, 2019 Apr 1. PMID 30617245, Free PMC Article
    2. Comparison of DNA decatenation by Escherichia coli topoisomerase IV and topoisomerase III: implications for non-equilibrium topology simplification. Seol Y, et al. Nucleic Acids Res, 2013 Apr. PMID 23460205, Free PMC Article
    3. A role for topoisomerase III in Escherichia coli chromosome segregation. Perez-Cheeks BA, et al. Mol Microbiol, 2012 Nov. PMID 23066834, Free PMC Article
    4. The type IA topoisomerase catalytic cycle: A normal mode analysis and molecular dynamics simulation. Xiong B, et al. Proteins, 2008 Jun. PMID 18186484
    5. Bacillus cereus DNA topoisomerase I and IIIalpha: purification, characterization and complementation of Escherichia coli TopoIII activity. Li Z, et al. Nucleic Acids Res, 2005. PMID 16192570, Free PMC Article

    See all (38) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Supercoiling, R-loops, Replication and the Functions of Bacterial Type 1A Topoisomerases.
      Title: Supercoiling, R-loops, Replication and the Functions of Bacterial Type 1A Topoisomerases.
    2. Study finding that both E. coli topo I and III control R-loop formation/accumulation, mostly to inhibit unregulated replication, may suggest that R-loops have been a problem early in the evolution of life. Furthermore, data show that E. coli cells can survive in the absence of type 1A topos, owing to the surproduction of topo IV that can relax excess negative supercoiling and prevent R-loop formation.
      Title: Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli.
    3. These results suggest that topoisomerase III participates in orderly and efficient chromosome segregation in E. coli.
      Title: A role for topoisomerase III in Escherichia coli chromosome segregation.
    4. Topo III unlinking rates were linearly related to the DNA collision frequency.
      Title: Comparison of DNA decatenation by Escherichia coli topoisomerase IV and topoisomerase III: implications for non-equilibrium topology simplification.
    5. Topoisomerase III relaxes DNA efficiently in fast processive runs, but with long pauses before relaxation runs.
      Title: Bacterial topoisomerase I and topoisomerase III relax supercoiled DNA via distinct pathways.
    6. N-terminus verified by Edman degradation on complete protein
      Title: Molecular cloning and DNA sequence analysis of Escherichia coli topB, the gene encoding topoisomerase III.
    7. These data are consistent with a role for topoisomerase III in disentangling recombination intermediates as an alternative to RuvABC to maintain the stability of the genome.
      Title: A role for topoisomerase III in a recombination pathway alternative to RuvABC.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables DNA topoisomerase type I (single strand cut, ATP-independent) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables isomerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific single stranded DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within DNA recombination IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA repair IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in DNA topological change IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within DNA topological change IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA topological change IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA-templated DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within chromosome separation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    is_active_in cytoplasmic replication fork IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasmic replication fork IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    DNA topoisomerase III
    NP_416277.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1844999..1846960 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416277.1 DNA topoisomerase III [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416277.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AA87FLU3
      Conserved Domains (1) summary
      PRK07726
      Location:1 → 642
      PRK07726; DNA topoisomerase 3
    Nucleotide Protein
    Heading Accession and Version

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