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    RPS27A ribosomal protein S27a [ Homo sapiens (human) ]

    Gene ID: 6233, updated on 7-Apr-2024

    Summary

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    Official Symbol
    RPS27Aprovided by HGNC
    Official Full Name
    ribosomal protein S27aprovided by HGNC
    Primary source
    HGNC:HGNC:10417
    See related
    Ensembl:ENSG00000143947 MIM:191343; AllianceGenome:HGNC:10417
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    UBC; S27A; eS31; CEP80; UBA80; HEL112; UBCEP1; UBCEP80
    Summary
    Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.[provided by RefSeq, Sep 2008]
    Expression
    Ubiquitous expression in ovary (RPKM 575.2), lymph node (RPKM 347.6) and 25 other tissues See more
    Orthologs
    mouse all
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    Genomic context

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    Location:
    2p16.1
    Exon count:
    7
    Annotation release Status Assembly Chr Location
    RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 2 NC_000002.12 (55231903..55235853)
    RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 2 NC_060926.1 (55229133..55233083)
    105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 2 NC_000002.11 (55459039..55462989)

    Chromosome 2 - NC_000002.12Genomic Context describing neighboring genes Neighboring gene reticulon 4 Neighboring gene uncharacterized LOC105374662 Neighboring gene Sharpr-MPRA regulatory region 4359 Neighboring gene OCT4-NANOG-H3K27ac hESC enhancer GRCh37_chr2:55360796-55361708 Neighboring gene CRISPRi-validated cis-regulatory element chr2.2087 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15775 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15776 Neighboring gene clathrin heavy chain linker domain containing 1 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 11496 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15777 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 15778 Neighboring gene CDPF1 pseudogene 1 Neighboring gene mitochondrial translational initiation factor 2 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:55496405-55497228 Neighboring gene Sharpr-MPRA regulatory regions 8685 and 822 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:55508487-55509380 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr2:55509381-55510272 Neighboring gene prolyl-tRNA synthetase associated domain containing 1, pseudogene

    Genomic regions, transcripts, and products

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    Go to nucleotide: Graphics FASTA GenBank

    Expression

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    • Project title: HPA RNA-seq normal tissues
    • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
    • BioProject: PRJEB4337
    • Publication: PMID 24309898
    • Analysis date: Wed Apr 4 07:08:55 2018

    Bibliography

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    Related articles in PubMed

    1. Multifaceted functions of RPS27a: An unconventional ribosomal protein. Luo J, et al. J Cell Physiol, 2023 Mar. PMID 36580426
    2. Rps27a might act as a controller of microglia activation in triggering neurodegenerative diseases. Khayer N, et al. PLoS One, 2020. PMID 32941527, Free PMC Article
    3. Interactions of antisera to different Chlamydia and Chlamydophila species with the ribosomal protein RPS27a correlate with impaired protein synthesis in a human choroid plexus papilloma cell line. Almamy A, et al. Immunol Res, 2017 Dec. PMID 28913776
    4. Identification and expression of MMSA-8, and its clinical significance in multiple myeloma. He R, et al. Oncol Rep, 2017 Jun. PMID 28498418, Free PMC Article
    5. RPS27a promotes proliferation, regulates cell cycle progression and inhibits apoptosis of leukemia cells. Wang H, et al. Biochem Biophys Res Commun, 2014 Apr 18. PMID 24680683

    See all (229) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination and DNA repair.
      Title: UBA80 and UBA52 fine-tune RNF168-dependent histone ubiquitination and DNA repair.
    2. RPS27a and RPL40, Which Are Produced as Ubiquitin Fusion Proteins, Are Not Essential for p53 Signalling.
      Title: RPS27a and RPL40, Which Are Produced as Ubiquitin Fusion Proteins, Are Not Essential for p53 Signalling.
    3. Multifaceted functions of RPS27a: An unconventional ribosomal protein.
      Title: Multifaceted functions of RPS27a: An unconventional ribosomal protein.
    4. PICT1 is critical for regulating the Rps27a-Mdm2-p53 pathway by microtubule polymerization inhibitor against cervical cancer.
      Title: PICT1 is critical for regulating the Rps27a-Mdm2-p53 pathway by microtubule polymerization inhibitor against cervical cancer.
    5. USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit.
      Title: USP16 counteracts mono-ubiquitination of RPS27a and promotes maturation of the 40S ribosomal subunit.
    6. The Ubiquitin Gene Expression Pattern and Sensitivity to UBB and UBC Knockdown Differentiate Primary 23132/87 and Metastatic MKN45 Gastric Cancer Cells.
      Title: The Ubiquitin Gene Expression Pattern and Sensitivity to UBB and UBC Knockdown Differentiate Primary 23132/87 and Metastatic MKN45 Gastric Cancer Cells.
    7. Rps27a might act as a controller of microglia activation in triggering neurodegenerative diseases.
      Title: Rps27a might act as a controller of microglia activation in triggering neurodegenerative diseases.
    8. MTG1 establishes a quality control checkpoint in mitoribosome assembly. In conclusion, MTG1 controls mitochondrial translation by coupling mtLSU assembly with intersubunit bridge formation using the intrinsic GEF activity acquired by the mtSSU through mS27, a unique occurrence in translational systems.
      Title: MTG1 couples mitoribosome large subunit assembly with intersubunit bridge formation.
    9. this study shows that interactions of antisera to different Chlamydia and Chlamydophila species with the ribosomal protein RPS27a correlate with impaired protein synthesis in a human choroid plexus papilloma cell line
      Title: Interactions of antisera to different Chlamydia and Chlamydophila species with the ribosomal protein RPS27a correlate with impaired protein synthesis in a human choroid plexus papilloma cell line.
    10. the full-length cDNA sequence of MMSA-8 was cloned in MM and it was hypothesized that MMSA-8 is MM-associated RPS27A transcript variant 1
      Title: Identification and expression of MMSA-8, and its clinical significance in multiple myeloma.
    Submit: New GeneRIF Correction See all GeneRIFs (18)

    Phenotypes

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    Find tests for this gene in the NIH Genetic Testing Registry (GTR)

    Review eQTL and phenotype association data in this region using PheGenI

    Variation

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    See variants in ClinVar

    See studies and variants in dbVar

    See Variation Viewer (GRCh37.p13)

    See Variation Viewer (GRCh38)

    Genotypes

    HIV-1 interactions

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    Protein interactions

    Protein Gene Interaction Pubs
    Rev rev HIV-1 Rev interacting protein, ribosomal protein S27A (RPS27A), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells PubMed

    Go to the HIV-1, Human Interaction Database

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    enables RNA binding HDA PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein tag activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables structural constituent of ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ubiquitin protein ligase binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    Process Evidence Code Pubs
    involved_in cytoplasmic translation IC
    Inferred by Curator
    more info
    		 PubMed 
    involved_in cytoplasmic translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in modification-dependent protein catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in protein ubiquitination IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in translation IC
    Inferred by Curator
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in cytosol TAS
    Traceable Author Statement
    more info
    		  
    located_in cytosolic ribosome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic small ribosomal subunit IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of cytosolic small ribosomal subunit NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    located_in endocytic vesicle membrane TAS
    Traceable Author Statement
    more info
    		  
    located_in endoplasmic reticulum membrane TAS
    Traceable Author Statement
    more info
    		  
    located_in endosome membrane TAS
    Traceable Author Statement
    more info
    		  
    located_in extracellular exosome HDA PubMed 
    located_in extracellular space HDA PubMed 
    located_in membrane HDA PubMed 
    located_in mitochondrial outer membrane TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleolus HDA PubMed 
    located_in nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    located_in nucleus HDA PubMed 
    is_active_in nucleus IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in plasma membrane TAS
    Traceable Author Statement
    more info
    		  
    part_of small-subunit processome IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in synapse IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in vesicle HDA PubMed 

    General protein information

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    Preferred Names
    ubiquitin-ribosomal protein eS31 fusion protein
    Names
    40S ribosomal protein S27a
    epididymis luminal protein 112
    ubiquitin C
    ubiquitin and ribosomal protein S27a
    ubiquitin carboxyl extension protein 80
    ubiquitin-40S ribosomal protein S27a
    ubiquitin-CEP80

    NCBI Reference Sequences (RefSeq)

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    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_017017.1 RefSeqGene

      Range
      5760..8925
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001135592.2 → NP_001129064.1  ubiquitin-ribosomal protein eS31 fusion protein precursor

      See identical proteins and their annotated locations for NP_001129064.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.
      Source sequence(s)
      AC012358, AK225079, DB505702
      Consensus CDS
      CCDS33202.1
      UniProtKB/Swiss-Prot
      P14798, P62979, Q9BQ77
      UniProtKB/TrEMBL
      B2RDW1, D6W5C9, Q5RKT7
      Related
      ENSP00000383981.3, ENST00000402285.7
      Conserved Domains (2) summary
      cd01803
      Location:1 → 76
      Ubl_ubiquitin; ubiquitin-like (Ubl) domain found in ubiquitin
      pfam01599
      Location:103 → 147
      Ribosomal_S27; Ribosomal protein S27a

    2. NM_001177413.1 → NP_001170884.1  ubiquitin-ribosomal protein eS31 fusion protein precursor

      See identical proteins and their annotated locations for NP_001170884.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2 and 3 encode the same protein.
      Source sequence(s)
      AC012358, BF678026, DA527860
      Consensus CDS
      CCDS33202.1
      UniProtKB/Swiss-Prot
      P14798, P62979, Q9BQ77
      UniProtKB/TrEMBL
      B2RDW1, D6W5C9, Q5RKT7
      Related
      ENSP00000385659.1, ENST00000404735.1
      Conserved Domains (2) summary
      cd01803
      Location:1 → 76
      Ubl_ubiquitin; ubiquitin-like (Ubl) domain found in ubiquitin
      pfam01599
      Location:103 → 147
      Ribosomal_S27; Ribosomal protein S27a

    3. NM_002954.6 → NP_002945.1  ubiquitin-ribosomal protein eS31 fusion protein precursor

      See identical proteins and their annotated locations for NP_002945.1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 encode the same protein.
      Source sequence(s)
      AC012358
      Consensus CDS
      CCDS33202.1
      UniProtKB/Swiss-Prot
      P14798, P62979, Q9BQ77
      UniProtKB/TrEMBL
      B2RDW1, D6W5C9, Q5RKT7
      Related
      ENSP00000272317.6, ENST00000272317.11
      Conserved Domains (2) summary
      cd01803
      Location:1 → 76
      Ubl_ubiquitin; ubiquitin-like (Ubl) domain found in ubiquitin
      pfam01599
      Location:103 → 147
      Ribosomal_S27; Ribosomal protein S27a

    RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh38.p14 Primary Assembly

    Genomic

    1. NC_000002.12 Reference GRCh38.p14 Primary Assembly

      Range
      55231903..55235853
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate T2T-CHM13v2.0

    Genomic

    1. NC_060926.1 Alternate T2T-CHM13v2.0

      Range
      55229133..55233083
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P62979.2 GenPept UniProtKB/Swiss-Prot:P62979

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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