WASp [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: WASpprovided by FlyBase
Primary source: FLYBASE:FBgn0024273
Locus tag: Dmel_CG1520
See related: AllianceGenome:FB:FBgn0024273
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG1520; D-WASP; Dm WASP; Dmel\CG1520; DmWASP; dWASP; N-WASP; wasp; Wasp; WASP; wsp; Wsp; WSP
Summary: Predicted to enable actin binding activity. Involved in several processes, including axon development; cytoskeleton organization; and epithelial cell differentiation. Located in several cellular components, including microvillus; neuromuscular junction; and rhabdomere. Part of protein-containing complex. Is expressed in several structures, including embryonic/larval central nervous system; embryonic/larval salivary gland common duct; muscle attachment site; neuromuscular junction; and presumptive embryonic/larval system. Human ortholog(s) of this gene implicated in Wiskott-Aldrich syndrome; X-linked severe congenital neutropenia; and thrombocytopenia. Orthologous to several human genes including WAS (WASP actin nucleation promoting factor). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 98E5-98E5; 3-97 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (28824506..28832024, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (24650228..24657746, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

along with controlling membrane binding, these autoregulatory interactions inhibit the ability of Nwk-SH3a to activate Wiskott-Aldrich syndrome protein (WASp)/actin related protein (Arp) 2/3-dependent actin filament ..we define structural rearrangements in Nwk that control F-BAR-membrane interactions as well as SH3 domain activities, and suggest that these two functions are tightly coordinated in vitro and in vivo.
Title: Coordinated autoinhibition of F-BAR domain membrane binding and WASp activation by Nervous Wreck.
Kette controls the ratio of the Arp2/3 activators Scar and WASp in fusion-competent myoblasts.
Title: Drosophila Kette coordinates myoblast junction dissolution and the ratio of Scar-to-WASp during myoblast fusion.
Data show that Tricornered Kinase (Trc) regulates the Wiskott-Aldrich syndrome protein (WASP) levels largely, by regulating the transcription of WASP.
Title: Tricornered Kinase Regulates Synapse Development by Regulating the Levels of Wiskott-Aldrich Syndrome Protein.
WASp has an essential role in the process of secretory cargo expulsion and integration of vesicular membranes with the apical plasma membrane.
Title: Arp2/3-mediated F-actin formation controls regulated exocytosis in vivo.
Study propose that Dock links cell adhesion in FCs and FCMs with either Scar- or Vrp1-WASp-dependent Arp2/3 activation.
Title: Dock mediates Scar- and WASp-dependent actin polymerization through interaction with cell adhesion molecules in founder cells and fusion-competent myoblasts.
Wash, WASp, SCAR, and Whamy are dynamically expressed throughout oogenesis and embryogenesis.
Title: Developmental expression of Drosophila Wiskott-Aldrich Syndrome family proteins.
Data suggest that the Wsp-Arp2/3-based machinery acts in the cyst cells to influence proper microfilament organization and to enable cyst attachment to the base of the testis.
Title: The WASp-based actin polymerization machinery is required in somatic support cells for spermatid maturation and release.
study demonstrates that the functional contribution of Wsp, the Drosophila homolog of the conserved actin nucleation-promoting factor (NPF) WASp, is essential for myoblast fusion during the formation of muscles of the adult fly
Title: The actin nucleator WASp is required for myoblast fusion during adult Drosophila myogenesis.
The studies identify Blown fuse protein that modulates the actin cytoskeletal dynamics by controlling the stability of the WASP-WIP complex.
Title: Competition between Blown fuse and WASP for WIP binding regulates the dynamics of WASP-dependent actin polymerization in vivo.
Data suggest that PI(4,5)P(2)- and WASP-mediated signaling at presynaptic termini controls actin-dependent synapse growth in a pathway at least in part in parallel to synaptic BMP signaling.
Title: WASP is activated by phosphatidylinositol-4,5-bisphosphate to restrict synapse growth in a pathway parallel to bone morphogenetic protein signaling.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables actin binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables small GTPase binding	IBA Inferred from Biological aspect of Ancestor more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Arp2/3 complex-mediated actin nucleation	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament organization	IEA Inferred from Electronic Annotation more info
involved_in apical constriction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in asymmetric cell division	TAS Traceable Author Statement more info	PubMed
involved_in axonal fasciculation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cell competition in a multicellular organism	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell fate commitment	TAS Traceable Author Statement more info	PubMed
involved_in chaeta morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in clathrin-dependent endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cuticle pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in epidermal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule bundle formation	IDA Inferred from Direct Assay more info	PubMed
involved_in muscle attachment	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in myoblast fusion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in myoblast fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of synaptic assembly at neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of actin filament polymerization	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of embryonic cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of synaptic assembly at neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
involved_in sensory organ development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in vesicle budding from membrane	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in microvillus	IMP Inferred from Mutant Phenotype more info	PubMed
located_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	IPI Inferred from Physical Interaction more info	PubMed
located_in rhabdomere	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: WASp

Names: CG1520-PA; CG1520-PB; CG1520-PC; CG1520-PD; WASP; WASp-PA; WASp-PB; WASp-PC; WASp-PD; Wiscott-Aldrich syndrome protein; Wiskott-Aldrich syndrome protein

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

28824506..28832024 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_143380.4 → NP_651637.1 WASp, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_651637.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VAT0

Related

FBpp0084721

Conserved Domains (3) summary

pfam00568
Location:31 → 140

WH1; WH1 domain

pfam00786
Location:233 → 291

PBD; P21-Rho-binding domain

pfam02205
Location:433 → 453

WH2; WH2 motif
NM_170378.2 → NP_733257.1 WASp, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_733257.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VAT0

Related

FBpp0084722, FBtr0085353

Conserved Domains (3) summary

pfam00568
Location:31 → 140

WH1; WH1 domain

pfam00786
Location:233 → 291

PBD; P21-Rho-binding domain

pfam02205
Location:433 → 453

WH2; WH2 motif
NM_001276116.1 → NP_001263045.1 WASp, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001263045.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VAT0

Conserved Domains (3) summary

pfam00568
Location:31 → 140

WH1; WH1 domain

pfam00786
Location:233 → 291

PBD; P21-Rho-binding domain

pfam02205
Location:433 → 453

WH2; WH2 motif
NM_176578.2 → NP_788755.1 WASp, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_788755.1

Status: REVIEWED

UniProtKB/TrEMBL

Q86B74

Related

FBpp0084723

Conserved Domains (3) summary

pfam00568
Location:3 → 107

WH1; WH1 domain

pfam00786
Location:200 → 258

PBD; P21-Rho-binding domain

pfam02205
Location:400 → 420

WH2; WH2 motif