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Series GSE49537 Query DataSets for GSE49537
Status Public on Apr 26, 2015
Title Transcriptomics Analyses of soybean leaves and roots sample during water-deficit
Organism Glycine max
Experiment type Expression profiling by array
Summary Microarray experiments were performed on the roots and leaves samples seperately using custom based Nimblgen platform (12plex). This is a time-course study. The plants were grown in controlled growth chamber condiditons ambiient to soybean growth and development using a hydrophonics system. The tissues were harvested after 30days of growth in six time points- 0min, 30mins, 1hour, 2hours, 3hours and 5hours of dehydration.
 
Overall design We used a hydrophonics system for the trancriptomics analysis of the soybean(William -82) root and leaves samples to study the reponse during water- deficit or drought. Plants were grown in controlled growth chamber conditions and harvested after 30days in six time-points. The plants were transferred to empty pots without touching to nullify any possibility of wounding and harvested subsequently. For 0mins, plants were taken out of the well-watered conditions and transfered to empty boxes and immediately harvested for both tisses and hence called 0-min dehydrated tissue. Similarly other time-points were processed (for instance, 2hrs of dehydration, plants were dehydrated for 2hours after being taken out from well-waterted pots and then harvested). Tissues for three biological replicates and three plants per biological replicates were collected for each time-point (n=9). RNA was islolated from both tissues using RNeasy Kit from Qiagen and quality was accessed with Nano-drop as well as with Agilent-Bioanalyzer using RNA-600 kit. 20ug of sample was processed at Micro Array Facility.
 
Contributor(s) Tripathi P, Rabara RC, Rushton PJ
Citation(s) 26123791, 26861168
Submission date Aug 05, 2013
Last update date Oct 16, 2019
Contact name Paul J Rushton
E-mail(s) paul.rushton@tamu.edu
Phone (972) 952 9246
Organization name Texas A&M AgriLife Research & Extension Center
Street address 17360 Coit Road
City Dallas
State/province Texas
ZIP/Postal code 75252
Country USA
 
Platforms (1)
GPL17531 NimbleGen Glycine Max Array [100526_Brach_MoGene_exp]
Samples (36)
GSM1201446 Leaf 0min-1
GSM1201447 Leaf 0min-2
GSM1201448 Leaf 0min-3
Relations
BioProject PRJNA214193

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE49537_RAW.tar 205.3 Mb (http)(custom) TAR (of CALLS, PAIR)
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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