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Series GSE65145 Query DataSets for GSE65145
Status Public on Oct 15, 2015
Title Rapamycin induces chromosome reorganization and increases cytokine production in normal human fibroblasts
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report the effects of Rapamycin treatment on the transcriptome of normal human dermal fibroblasts isolated from foreskin (designated 2DD). We sequenced mRNA from 2 replicates of proliferative (PRO) quiescent (QUI, serum starved) or treated with 500nM Rapamycin for 5 days (RAP). Comparative analyses with PRO transcripts a baseline indicate that genes that changed expression from Rapamycin treated fibroblasts are significantly different from those of quiescence cells. Rapamycin treated cells showed a significant enrichment for cytokines from the Il-6 cascade.
Overall design Examination of mRNAs from proliferative, quiescent (serum starvation) and Rapamycin (5oonM, 5days) treated 2DD normal human dermal/foreskin fibroblasts.
Contributor(s) Mitchell JA, Eskiw CE
Citation(s) 26473061
Brett Trost, Catherine A. Moir, Zoe E. Gillespie, Anthony Kusalik, Jennifer A. Mitchell, Christopher H. Eskiw. Concordance between RNA-sequencing data and DNA microarray data in transcriptome analysis of proliferative and quiescent fibroblasts. R Soc Open Sci. 30 Sept 2015
Submission date Jan 21, 2015
Last update date Oct 29, 2019
Contact name Christopher H. Eskiw
Phone 306-966-2454
Organization name University of Saskatchewan
Department Food and Bioproduct Science
Lab Eskiw Lab
Street address 51 Campus Drive
City Saskatoon
State/province SK
ZIP/Postal code S7N 5A8
Country Canada
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (6)
GSM1588051 proliferative fibroblast 1
GSM1588052 proliferative fibroblast 2
GSM1588053 queiscent fibroblast 1
BioProject PRJNA273277
SRA SRP052706

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Supplementary file Size Download File type/resource
GSE65145_RAW.tar 9.7 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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