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| Status |
Public on Oct 15, 2015 |
| Title |
Rapamycin induces chromosome reorganization and increases cytokine production in normal human fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the effects of Rapamycin treatment on the transcriptome of normal human dermal fibroblasts isolated from foreskin (designated 2DD). We sequenced mRNA from 2 replicates of proliferative (PRO) quiescent (QUI, serum starved) or treated with 500nM Rapamycin for 5 days (RAP). Comparative analyses with PRO transcripts a baseline indicate that genes that changed expression from Rapamycin treated fibroblasts are significantly different from those of quiescence cells. Rapamycin treated cells showed a significant enrichment for cytokines from the Il-6 cascade.
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| |
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| Overall design |
Examination of mRNAs from proliferative, quiescent (serum starvation) and Rapamycin (5oonM, 5days) treated 2DD normal human dermal/foreskin fibroblasts.
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| |
|
| Contributor(s) |
Mitchell JA, Eskiw CE |
| Citation(s) |
26473061 |
|
Brett Trost, Catherine A. Moir, Zoe E. Gillespie, Anthony Kusalik, Jennifer A. Mitchell, Christopher H. Eskiw. Concordance between RNA-sequencing data and DNA microarray data in transcriptome analysis of proliferative and quiescent fibroblasts. R Soc Open Sci. 30 Sept 2015 http://dx.doi.org/10.1098/rsos.150402
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| |
| Submission date |
Jan 21, 2015 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Christopher H. Eskiw |
| E-mail(s) |
c.eskiw@usask.ca
|
| Phone |
306-966-2454
|
| Organization name |
University of Saskatchewan
|
| Department |
Food and Bioproduct Science
|
| Lab |
Eskiw Lab
|
| Street address |
51 Campus Drive
|
| City |
Saskatoon |
| State/province |
SK |
| ZIP/Postal code |
S7N 5A8 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA273277 |
| SRA |
SRP052706 |
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