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Sample GSM2 Query DataSets for GSM2
Status Public on Sep 28, 2000
Title HCMV-infected foreskin fibroblasts
Sample type SAGE
Anchor NlaIII
Tag Count 9497
Tag Length 10
 
Source name mRNA of HCMV-infected human foreskin fibroblasts
Organism Homo sapiens
Extracted molecule total RNA
 
Description Primary human foreskin fibroblasts at passages 15-18, cultured in Eagles Minimal Essential Medium (MEM) supplemented with penicillin and 10% fetal calf serum (FCS).
Confluent cells serum starved for 3 days with MEM containing 1% FCS.
85% of cells in G0/G1 cell cycle phase.
Cells infected at a multiplicity of infection (m.o.i.) of 5 with human cytomegalovirus (HCMV) strain AD169 infectious particles (virions) for 90 min at 37┬░Celsius.
mRNA isolated using a direct mRNA extraction kit.
Keywords = human cytomegalovirus, AD169, virions, HCMV-infected foreskin fibroblasts
 
Submission date Sep 28, 2000
Last update date Jun 27, 2016
Contact name Marc Kenzelmann
E-mail(s) m.kenzelmann@dkfz.de
Phone +41 31 632 3251
Fax +41 31 632 3550
Organization name Institute of Medical Microbiology, University of Bern
Department Molecular Genome Analysis
Street address Friedbuhlstrasse 51
City Bern
ZIP/Postal code 3010
Country Switzerland
 
Platform ID GPL4
Series (1)
GSE506 Fibroblast cells immediate-early after human cytomegalovirus infection

Data table header descriptions
TAG Ten base SAGE tag,
COUNT TAG NUMBER
TPM tags per million

Data table
TAG COUNT TPM
AAAAAAAAAA 16 1684.74
AAAAAAAAAC 1 105.30
AAAAAAAAAG 1 105.30
AAAAAAAAAT 1 105.30
AAAAAAATTT 2 210.59
AAAAAACCCT 1 105.30
AAAAAAGCAG 1 105.30
AAAAAAGCTT 1 105.30
AAAAACCATA 1 105.30
AAAAACCCAT 1 105.30
AAAAACCCTT 1 105.30
AAAAACTGGA 1 105.30
AAAAAGCAGA 1 105.30
AAAAATAAAA 2 210.59
AAAAATAAAG 2 210.59
AAAAATATAA 1 105.30
AAAAATTTTC 1 105.30
AAAACAAAAT 1 105.30
AAAACAATGG 1 105.30
AAAACATCAT 1 105.30

Total number of rows: 5626

Table truncated, full table size 110 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Processed data are available on Series record

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